Construct: sgRNA BRDN0001146726
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGACCGAGTTCATGTGTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGR (2268)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75719
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
27615439 |
- |
FGR |
NNNACCGAGTTCATGTGTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
17846663 |
- |
SERGEF |
NNNACAGTGTTCATGTGTCA |
NGG |
2 |
0.398 |
Tier II |
| 3 |
NC_000006.12 |
6 |
63977734 |
+ |
EYS |
NNNACCGAGTTTGTGTGTCA |
NGG |
2 |
0.3512 |
Tier II |
| 4 |
NC_000011.10 |
11 |
126437961 |
- |
KIRREL3 |
NNNACCGTGTACATGTGTCA |
NGG |
2 |
0.3214 |
Tier II |
| 5 |
NC_000016.10 |
16 |
956103 |
+ |
LMF1 |
NNNTCCGAGTTCATGTCTCA |
NGG |
2 |
0.1497 |
Tier II |
| 6 |
NC_000016.10 |
16 |
956160 |
+ |
LMF1 |
NNNTCCGAGTTCATGTCTCA |
NGG |
2 |
0.1497 |
Tier II |
| 7 |
NC_000023.11 |
X |
143515753 |
- |
SPANXN3 |
NNNACCGAGTTCATGTGGCC |
NGG |
2 |
0.0758 |
Tier II |
| 8 |
NC_000016.10 |
16 |
85565049 |
- |
GSE1 |
NNNATCGAGTTCATGTGTCA |
NCG |
2 |
0.0682 |
Tier II |
| 9 |
NC_000004.12 |
4 |
24003552 |
+ |
PPARGC1A |
NNNACCGATTTCAGGTGTCA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000006.12 |
6 |
63977734 |
+ |
LOC107986608 |
NNNACCGAGTTTGTGTGTCA |
NGG |
2 |
0.3512 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
123130470 |
- |
Ppie |
NNNCCCCAGTTCATGTGTCA |
NGG |
2 |
0.2426 |
Tier I |
| 2 |
NC_000075.6 |
9 |
36996888 |
+ |
Pknox2 |
NNNACCGAGTTCATGAGACA |
NGG |
2 |
0.6061 |
Tier II |
| 3 |
NC_000070.6 |
4 |
59363239 |
+ |
Susd1 |
NNNACAGAGTTCGTGTGTCA |
NGG |
2 |
0.6056 |
Tier II |
| 4 |
NC_000080.6 |
14 |
32556913 |
- |
Ercc6 |
NNNACCGAGCTCATGTGTCA |
NGG |
1 |
0.5333 |
Tier II |
| 5 |
NC_000078.6 |
12 |
116052605 |
- |
Zfp386 |
NNNACCCAGTTTATGTGTCA |
NGG |
2 |
0.3702 |
Tier II |
| 6 |
NC_000071.6 |
5 |
123777639 |
- |
Kntc1 |
NNNACCGAGTTCATGTGTCA |
NTG |
1 |
0.039 |
Tier II |
| 7 |
NC_000068.7 |
2 |
162459895 |
- |
Ptprt |
NNNACCGGGTTCATGTGTCA |
NTG |
2 |
0.0286 |
Tier II |
| 8 |
NC_000082.6 |
16 |
14435063 |
+ |
Abcc1 |
NNNCCCGAGTTCATGTGTCA |
NTG |
2 |
0.0138 |
Tier II |
| 9 |
NC_000071.6 |
5 |
28210073 |
- |
Cnpy1 |
NNNACCGAGTTCATTTGTCA |
NGC |
2 |
0.0032 |
Tier II |
| 10 |
NC_000073.6 |
7 |
94193940 |
+ |
Gm32647 |
NNNACAGAGTTGATGTGTCA |
NGG |
2 |
0.4127 |
Tier III |
Other clones with same target sequence:
(none)
