Construct: sgRNA BRDN0001146728
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGCCAGTACACATGTACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK7 (5754)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78024
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
43129080 |
- |
PTK7 |
NNNGCCAGTACACATGTACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
82961474 |
+ |
TMTC2 |
NNNCCGAGTACACATGTACC |
NGG |
2 |
0.2647 |
Tier II |
| 3 |
NC_000010.11 |
10 |
125983661 |
+ |
FANK1 |
NNNGGCAGTACAAATGTACC |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000002.12 |
2 |
50388435 |
- |
NRXN1 |
NNNGCCAGTACACATGTACT |
NGA |
2 |
0.0208 |
Tier II |
| 5 |
NC_000007.14 |
7 |
94564194 |
+ |
CASD1 |
NNNGCCAGTAGACATGTACC |
NGA |
2 |
0.0174 |
Tier II |
| 6 |
NC_000012.12 |
12 |
111268482 |
+ |
CUX2 |
NNNGCCAGTACACATGTGCC |
NGC |
2 |
0.0089 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
46591700 |
+ |
Ptk7 |
NNNGCCAGTACACGTGCACC |
NGG |
2 |
0.3294 |
Tier I |
| 2 |
NC_000086.7 |
X |
134693552 |
+ |
Armcx4 |
NNNGCCAGTGCACATGAACC |
NGG |
2 |
0.2963 |
Tier I |
| 3 |
NC_000079.6 |
13 |
37949296 |
+ |
Rreb1 |
NNNCCCAGTACACATGCACC |
NGG |
2 |
0.3737 |
Tier II |
| 4 |
NC_000071.6 |
5 |
101875148 |
- |
Wdfy3 |
NNNGTCTGTACACATGTACC |
NGG |
2 |
0.2784 |
Tier II |
| 5 |
NC_000072.6 |
6 |
82971114 |
+ |
M1ap |
NNNGCCAGTACACATGAAAC |
NGG |
2 |
0.2286 |
Tier II |
| 6 |
NC_000073.6 |
7 |
110275572 |
+ |
Swap70 |
NNNGGCAGTACAGATGTACC |
NGG |
2 |
0.0818 |
Tier II |
| 7 |
NC_000075.6 |
9 |
57136443 |
- |
Man2c1 |
NNNGCCAGGACACAGGTACC |
NGG |
2 |
0.031 |
Tier II |
| 8 |
NC_000084.6 |
18 |
39052821 |
- |
Arhgap26 |
NNNGGCAGTACACAGGTACC |
NGG |
2 |
0.03 |
Tier II |
| 9 |
NC_000067.6 |
1 |
128008147 |
+ |
Zranb3 |
NNNGCCTGTACACATGTACC |
NTG |
2 |
0.017 |
Tier II |
| 10 |
NC_000073.6 |
7 |
124263256 |
+ |
Hs3st4 |
NNNCCCAGTACACATGTACC |
NGT |
2 |
0.0085 |
Tier II |
| 11 |
NC_000071.6 |
5 |
37656693 |
- |
Stk32b |
NNNGCCAGTGCACATCTACC |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000070.6 |
4 |
46334724 |
+ |
Gm12446 |
NNNGCCAGTACACATGAGCC |
NGG |
2 |
0.2133 |
Tier III |
| 13 |
NC_000082.6 |
16 |
12846884 |
+ |
1700003L19Rik |
NNNGCCTGTACACATGTGCC |
NGG |
2 |
0.175 |
Tier III |
Other clones with same target sequence:
(none)
