Construct: sgRNA BRDN0001146731
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCACATCACCTACACTACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAST2 (23139)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76937
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
45959444 |
+ |
MAST2 |
NNNCATCACCTACACTACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
116224682 |
- |
TES |
NNNCATCAGCTACACTAACA |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000004.12 |
4 |
142599158 |
+ |
INPP4B |
NNNCATCACCTACACCACAA |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000021.9 |
21 |
43934089 |
- |
AGPAT3 |
NNNCATCACCCAAACTACCA |
NGG |
2 |
0.2564 |
Tier II |
| 5 |
NC_000001.11 |
1 |
51525669 |
+ |
OSBPL9 |
NNNCACCACCTCCACTACCA |
NGG |
2 |
0.2392 |
Tier II |
| 6 |
NC_000001.11 |
1 |
32279160 |
- |
LCK |
NNNCATCATCTACACTCCCA |
NGG |
2 |
0.1544 |
Tier II |
| 7 |
NC_000008.11 |
8 |
105796653 |
+ |
ZFPM2 |
NNNCATCTCCTTCACTACCA |
NGG |
2 |
0.1429 |
Tier II |
| 8 |
NC_000023.11 |
X |
68711041 |
+ |
STARD8 |
NNNCATCACCTAGACTACCA |
NTG |
2 |
0.0053 |
Tier II |
| 9 |
NC_000020.11 |
20 |
17840500 |
- |
LOC107985440 |
NNNCATCACATACACTAACA |
NGG |
2 |
0.4667 |
Tier III |
| 10 |
NC_000016.10 |
16 |
51516819 |
+ |
LOC102723323 |
NNNCCTCACCTACACCACCA |
NGG |
2 |
0.3333 |
Tier III |
| 11 |
NC_000004.12 |
4 |
142599158 |
+ |
LOC101927613 |
NNNCATCACCTACACCACAA |
NGG |
2 |
0.2857 |
Tier III |
| 12 |
NC_000001.11 |
1 |
51525669 |
+ |
EPS15-AS1 |
NNNCACCACCTCCACTACCA |
NGG |
2 |
0.2392 |
Tier III |
| 13 |
NC_000008.11 |
8 |
105796653 |
+ |
ZFPM2-AS1 |
NNNCATCTCCTTCACTACCA |
NGG |
2 |
0.1429 |
Tier III |
| 14 |
NC_000010.11 |
10 |
27308125 |
+ |
ODAD2P1 |
NNNCATCACCTGCACTTCCA |
NGG |
2 |
0.0963 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
116353007 |
- |
Mast2 |
NNNCATCACCTACGCTACCA |
NGG |
1 |
0.4667 |
Tier I |
| 2 |
NC_000077.6 |
11 |
118095004 |
- |
Dnah17 |
NNNCATCACCTGGACTACCA |
NGG |
2 |
0.0985 |
Tier I |
| 3 |
NC_000069.6 |
3 |
105904852 |
+ |
Adora3 |
NNNCATCACCTACATCACCA |
NGG |
2 |
0.0444 |
Tier I |
| 4 |
NC_000081.6 |
15 |
43091176 |
+ |
Rspo2 |
NNNCATCACCTAAACTACCA |
NGG |
1 |
0.3846 |
Tier II |
| 5 |
NC_000078.6 |
12 |
116514178 |
+ |
Ptprn2 |
NNNCAGCACCCACACTACCA |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000082.6 |
16 |
74491479 |
+ |
Robo2 |
NNNCATCATCTACACTACAA |
NGG |
2 |
0.375 |
Tier II |
| 7 |
NC_000069.6 |
3 |
55157701 |
+ |
Ccdc169 |
NNNCCTCACCCACACTACCA |
NGG |
2 |
0.3333 |
Tier II |
| 8 |
NC_000084.6 |
18 |
12459003 |
+ |
Lama3 |
NNNCCTCACCCACACTACCA |
NGG |
2 |
0.3333 |
Tier II |
| 9 |
NC_000079.6 |
13 |
46834288 |
+ |
Kif13a |
NNNCATGACCTACACTATCA |
NGG |
2 |
0.3025 |
Tier II |
| 10 |
NC_000070.6 |
4 |
152361767 |
- |
Chd5 |
NNNCATCCCCTACACCACCA |
NGG |
2 |
0.2857 |
Tier II |
| 11 |
NC_000076.6 |
10 |
43492102 |
- |
Bend3 |
NNNCATCACCAAGACTACCA |
NGG |
2 |
0.1023 |
Tier II |
| 12 |
NC_000074.6 |
8 |
114533286 |
- |
Wwox |
NNNCTTCACCTACCCTACCA |
NGG |
2 |
0.0779 |
Tier II |
| 13 |
NC_000072.6 |
6 |
52006827 |
+ |
Skap2 |
NNNCATCACCTCCACTCCCA |
NGG |
2 |
0.0464 |
Tier II |
| 14 |
NC_000069.6 |
3 |
105904852 |
+ |
Tmigd3 |
NNNCATCACCTACATCACCA |
NGG |
2 |
0.0444 |
Tier II |
| 15 |
NC_000075.6 |
9 |
57678259 |
+ |
Cyp1a2 |
NNNCATCACATACACTACCA |
NTG |
2 |
0.0338 |
Tier II |
| 16 |
NC_000074.6 |
8 |
111557676 |
+ |
Znrf1 |
NNNCATCACCTACATTGCCA |
NGG |
2 |
0.0118 |
Tier II |
| 17 |
NC_000079.6 |
13 |
50927033 |
+ |
1700048B10Rik |
NNNCATCCCCTCCACTACCA |
NGG |
2 |
0.1128 |
Tier III |
| 18 |
NC_000079.6 |
13 |
51316001 |
+ |
Gm46414 |
NNNCATCACCTACAGCACCA |
NGG |
2 |
0.0333 |
Tier III |
| 19 |
NC_000083.6 |
17 |
21920876 |
+ |
Gm6540 |
NNNCATCACCTACACTGCGA |
NGG |
2 |
0.0221 |
Tier III |
Other clones with same target sequence:
(none)
