Construct: sgRNA BRDN0001146734

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCCAGAGCCCCAGTTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA8 (2046)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76529

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 22597375 + EPHA8 NNNAGAGCCCCAGTTCTATG NGG 0 1.0 Tier I
2 NC_000016.10 16 1334396 - BAIAP3 NNNACACCCCCAGTTCTATG NGG 2 0.5402 Tier II
3 NC_000009.12 9 115061293 - TNC NNNAAAGCCCCAGATCTATG NGG 2 0.5365 Tier II
4 NC_000004.12 4 51855080 - DCUN1D4 NNNAGAGCCCAAGTTCCATG NGG 2 0.5294 Tier II
5 NC_000014.9 14 100445056 - WDR25 NNNAGAGGCCCAGTTCCATG NGG 2 0.4538 Tier II
6 NC_000023.11 X 32432237 + DMD NNNAGAACCCCAGTTTTATG NGG 2 0.3077 Tier II
7 NC_000006.12 6 110657595 - CDK19 NNNAGAGCCCCAGTACTTTG NGG 2 0.2895 Tier II
8 NC_000003.12 3 59938889 + FHIT NNNTGAGCCCCAGTTTTATG NGG 2 0.1958 Tier II
9 NC_000021.9 21 45871801 + PCBP3 NNNAGAGCCCCAGTTCCATG NAG 2 0.183 Tier II
10 NC_000001.11 1 178377582 - RASAL2 NNNAGAGGCCCAGTCCTATG NGG 2 0.1753 Tier II
11 NC_000012.12 12 125612070 - TMEM132B NNNAGAGCCCCAGATCTATG NAG 2 0.1605 Tier II
12 NC_000012.12 12 6457257 + TAPBPL NNNAGAGCCCCATTTCTTTG NGG 2 0.15 Tier II
13 NC_000001.11 1 201906419 - LMOD1 NNNAGAGCCCCAGTTCTTGG NGG 2 0.125 Tier II
14 NC_000008.11 8 1335962 - DLGAP2 NNNAAAGCCCCAGTTCTATG NGC 2 0.0193 Tier II
15 NC_000001.11 1 183507358 + SMG7 NNNAAAGCCCCAGTTCTATG NGT 2 0.014 Tier II
16 NC_000013.11 13 20596817 - IFT88 NNNAAAGCCCCAGTTCTATG NGT 2 0.014 Tier II
17 NC_000012.12 12 13560996 - GRIN2B NNNAGAGCCCCAGGTCTGTG NGG 2 0.0 Tier II
18 NC_000021.9 21 39361240 - LOC105372804 NNNAGAGCACAAGTTCTATG NGG 2 0.6429 Tier III
19 NC_000006.12 6 105617869 - LINC02836 NNNAGAGCCCAAGTTCTGTG NGG 2 0.3 Tier III
20 NC_000018.10 18 73177433 - LINC02864 NNNAGTGCCCCAGTTCTGTG NGG 2 0.2857 Tier III
21 NC_000009.12 9 108317008 + LOC105376214 NNNAGAGCTCCAGTTCTAAG NGG 2 0.25 Tier III
22 NC_000016.10 16 80319176 + DYNLRB2-AS1 NNNCGAGCCCCAGTTCTAGG NGG 2 0.0882 Tier III
23 NC_000017.11 17 27991148 + LINC01992 NNNAGAGCCCCAGATCTATG NCG 2 0.0663 Tier III
24 NC_000008.11 8 1335962 - LOC124901869 NNNAAAGCCCCAGTTCTATG NGC 2 0.0193 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 3544892 + Ppp6r3 NNNAGAACTCCAGTTCTATG NGG 2 0.875 Tier II
2 NC_000077.6 11 16833214 - Egfr NNNAGAGCTCCAGTTCTATG NGG 1 0.875 Tier II
3 NC_000071.6 5 21484257 + Fbxl13 NNNAGAGCTTCAGTTCTATG NGG 2 0.8235 Tier II
4 NC_000071.6 5 21484257 + Fam185a NNNAGAGCTTCAGTTCTATG NGG 2 0.8235 Tier II
5 NC_000082.6 16 16109228 - Spidr NNNACAGCCCCAGTTCTATA NGG 2 0.7366 Tier II
6 NC_000071.6 5 103459198 - Ptpn13 NNNAGGGCCCCAGTTCTATG NGG 1 0.7143 Tier II
7 NC_000067.6 1 136542015 + Kif14 NNNAGATCCTCAGTTCTATG NGG 2 0.5378 Tier II
8 NC_000074.6 8 88583696 + Nkd1 NNNAGAGCCCCAGTTCTATC NGG 1 0.4286 Tier II
9 NC_000075.6 9 72497389 + Tex9 NNNAGAGCCCAAGTTCTTTG NGG 2 0.375 Tier II
10 NC_000073.6 7 127003200 + Mvp NNNAGAGCCACAGTTCTGTG NGG 2 0.3467 Tier II
11 NC_000082.6 16 36655973 + Cd86 NNNAGAGCTGCAGTTCTATG NGG 2 0.3403 Tier II
12 NC_000079.6 13 89239217 - Edil3 NNNAGAACCCCAGTTCTACG NGG 2 0.2759 Tier II
13 NC_000076.6 10 23117553 - Eya4 NNNAGAGCCCAATTTCTATG NGG 2 0.225 Tier II
14 NC_000074.6 8 14835103 - Dlgap2 NNNATAGCCCCAGTTCCATG NGG 2 0.2118 Tier II
15 NC_000074.6 8 38329019 + Sgcz NNNAGACCCCCAGTTCTAGG NGG 2 0.1719 Tier II
16 NC_000071.6 5 117749769 + Ksr2 NNNAGAGCCACAGTTCTCTG NGG 2 0.1651 Tier II
17 NC_000081.6 15 3374586 + Ghr NNNAGAGCCCCAGTTTAATG NGG 2 0.1641 Tier II
18 NC_000073.6 7 45684949 + Sec1 NNNAGATCCCCAGTCCTATG NGG 2 0.1558 Tier II
19 NC_000073.6 7 45684949 + Ntn5 NNNAGATCCCCAGTCCTATG NGG 2 0.1558 Tier II
20 NC_000072.6 6 8016149 + Col28a1 NNNAGAGCCGCTGTTCTATG NGG 2 0.1296 Tier II
21 NC_000068.7 2 28718850 - Ak8 NNNAGAGCCCCAGCTCTAGG NGG 2 0.0714 Tier II
22 NC_000076.6 10 81230311 - Atcay NNNAGAGCCCCAGTTCTCGG NGG 2 0.0476 Tier II
23 NC_000071.6 5 74059684 + Usp46 NNNAGAGCCCAAGTGCTATG NGG 2 0.0375 Tier II
24 NC_000071.6 5 142597907 + Mmd2 NNNAGAGCCCCAGTGCTATG NAG 2 0.013 Tier II
25 NC_000068.7 2 60802284 + Rbms1 NNNAAAGCCCCAGGTCTATG NGG 2 0.0 Tier II
26 NC_000075.6 9 72497389 + Gm33004 NNNAGAGCCCAAGTTCTTTG NGG 2 0.375 Tier III
27 NC_000081.6 15 3374586 + Gm41254 NNNAGAGCCCCAGTTTAATG NGG 2 0.1641 Tier III
28 NC_000072.6 6 81992196 - Gm20383 NNNGGAGCCCCAGTTCTATG NAG 2 0.162 Tier III
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Other clones with same target sequence:

(none)