Construct: sgRNA BRDN0001146734
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCAGAGCCCCAGTTCTATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA8 (2046)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76529
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000001.11 |
1 |
22597375 |
+ |
EPHA8 |
NNNAGAGCCCCAGTTCTATG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000016.10 |
16 |
1334396 |
- |
BAIAP3 |
NNNACACCCCCAGTTCTATG |
NGG |
2 |
0.5402 |
Tier II |
3 |
NC_000009.12 |
9 |
115061293 |
- |
TNC |
NNNAAAGCCCCAGATCTATG |
NGG |
2 |
0.5365 |
Tier II |
4 |
NC_000004.12 |
4 |
51855080 |
- |
DCUN1D4 |
NNNAGAGCCCAAGTTCCATG |
NGG |
2 |
0.5294 |
Tier II |
5 |
NC_000014.9 |
14 |
100445056 |
- |
WDR25 |
NNNAGAGGCCCAGTTCCATG |
NGG |
2 |
0.4538 |
Tier II |
6 |
NC_000023.11 |
X |
32432237 |
+ |
DMD |
NNNAGAACCCCAGTTTTATG |
NGG |
2 |
0.3077 |
Tier II |
7 |
NC_000006.12 |
6 |
110657595 |
- |
CDK19 |
NNNAGAGCCCCAGTACTTTG |
NGG |
2 |
0.2895 |
Tier II |
8 |
NC_000003.12 |
3 |
59938889 |
+ |
FHIT |
NNNTGAGCCCCAGTTTTATG |
NGG |
2 |
0.1958 |
Tier II |
9 |
NC_000021.9 |
21 |
45871801 |
+ |
PCBP3 |
NNNAGAGCCCCAGTTCCATG |
NAG |
2 |
0.183 |
Tier II |
10 |
NC_000001.11 |
1 |
178377582 |
- |
RASAL2 |
NNNAGAGGCCCAGTCCTATG |
NGG |
2 |
0.1753 |
Tier II |
11 |
NC_000012.12 |
12 |
125612070 |
- |
TMEM132B |
NNNAGAGCCCCAGATCTATG |
NAG |
2 |
0.1605 |
Tier II |
12 |
NC_000012.12 |
12 |
6457257 |
+ |
TAPBPL |
NNNAGAGCCCCATTTCTTTG |
NGG |
2 |
0.15 |
Tier II |
13 |
NC_000001.11 |
1 |
201906419 |
- |
LMOD1 |
NNNAGAGCCCCAGTTCTTGG |
NGG |
2 |
0.125 |
Tier II |
14 |
NC_000008.11 |
8 |
1335962 |
- |
DLGAP2 |
NNNAAAGCCCCAGTTCTATG |
NGC |
2 |
0.0193 |
Tier II |
15 |
NC_000001.11 |
1 |
183507358 |
+ |
SMG7 |
NNNAAAGCCCCAGTTCTATG |
NGT |
2 |
0.014 |
Tier II |
16 |
NC_000013.11 |
13 |
20596817 |
- |
IFT88 |
NNNAAAGCCCCAGTTCTATG |
NGT |
2 |
0.014 |
Tier II |
17 |
NC_000012.12 |
12 |
13560996 |
- |
GRIN2B |
NNNAGAGCCCCAGGTCTGTG |
NGG |
2 |
0.0 |
Tier II |
18 |
NC_000021.9 |
21 |
39361240 |
- |
LOC105372804 |
NNNAGAGCACAAGTTCTATG |
NGG |
2 |
0.6429 |
Tier III |
19 |
NC_000006.12 |
6 |
105617869 |
- |
LINC02836 |
NNNAGAGCCCAAGTTCTGTG |
NGG |
2 |
0.3 |
Tier III |
20 |
NC_000018.10 |
18 |
73177433 |
- |
LINC02864 |
NNNAGTGCCCCAGTTCTGTG |
NGG |
2 |
0.2857 |
Tier III |
21 |
NC_000009.12 |
9 |
108317008 |
+ |
LOC105376214 |
NNNAGAGCTCCAGTTCTAAG |
NGG |
2 |
0.25 |
Tier III |
22 |
NC_000016.10 |
16 |
80319176 |
+ |
DYNLRB2-AS1 |
NNNCGAGCCCCAGTTCTAGG |
NGG |
2 |
0.0882 |
Tier III |
23 |
NC_000017.11 |
17 |
27991148 |
+ |
LINC01992 |
NNNAGAGCCCCAGATCTATG |
NCG |
2 |
0.0663 |
Tier III |
24 |
NC_000008.11 |
8 |
1335962 |
- |
LOC124901869 |
NNNAAAGCCCCAGTTCTATG |
NGC |
2 |
0.0193 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000085.6 |
19 |
3544892 |
+ |
Ppp6r3 |
NNNAGAACTCCAGTTCTATG |
NGG |
2 |
0.875 |
Tier II |
2 |
NC_000077.6 |
11 |
16833214 |
- |
Egfr |
NNNAGAGCTCCAGTTCTATG |
NGG |
1 |
0.875 |
Tier II |
3 |
NC_000071.6 |
5 |
21484257 |
+ |
Fbxl13 |
NNNAGAGCTTCAGTTCTATG |
NGG |
2 |
0.8235 |
Tier II |
4 |
NC_000071.6 |
5 |
21484257 |
+ |
Fam185a |
NNNAGAGCTTCAGTTCTATG |
NGG |
2 |
0.8235 |
Tier II |
5 |
NC_000082.6 |
16 |
16109228 |
- |
Spidr |
NNNACAGCCCCAGTTCTATA |
NGG |
2 |
0.7366 |
Tier II |
6 |
NC_000071.6 |
5 |
103459198 |
- |
Ptpn13 |
NNNAGGGCCCCAGTTCTATG |
NGG |
1 |
0.7143 |
Tier II |
7 |
NC_000067.6 |
1 |
136542015 |
+ |
Kif14 |
NNNAGATCCTCAGTTCTATG |
NGG |
2 |
0.5378 |
Tier II |
8 |
NC_000074.6 |
8 |
88583696 |
+ |
Nkd1 |
NNNAGAGCCCCAGTTCTATC |
NGG |
1 |
0.4286 |
Tier II |
9 |
NC_000075.6 |
9 |
72497389 |
+ |
Tex9 |
NNNAGAGCCCAAGTTCTTTG |
NGG |
2 |
0.375 |
Tier II |
10 |
NC_000073.6 |
7 |
127003200 |
+ |
Mvp |
NNNAGAGCCACAGTTCTGTG |
NGG |
2 |
0.3467 |
Tier II |
11 |
NC_000082.6 |
16 |
36655973 |
+ |
Cd86 |
NNNAGAGCTGCAGTTCTATG |
NGG |
2 |
0.3403 |
Tier II |
12 |
NC_000079.6 |
13 |
89239217 |
- |
Edil3 |
NNNAGAACCCCAGTTCTACG |
NGG |
2 |
0.2759 |
Tier II |
13 |
NC_000076.6 |
10 |
23117553 |
- |
Eya4 |
NNNAGAGCCCAATTTCTATG |
NGG |
2 |
0.225 |
Tier II |
14 |
NC_000074.6 |
8 |
14835103 |
- |
Dlgap2 |
NNNATAGCCCCAGTTCCATG |
NGG |
2 |
0.2118 |
Tier II |
15 |
NC_000074.6 |
8 |
38329019 |
+ |
Sgcz |
NNNAGACCCCCAGTTCTAGG |
NGG |
2 |
0.1719 |
Tier II |
16 |
NC_000071.6 |
5 |
117749769 |
+ |
Ksr2 |
NNNAGAGCCACAGTTCTCTG |
NGG |
2 |
0.1651 |
Tier II |
17 |
NC_000081.6 |
15 |
3374586 |
+ |
Ghr |
NNNAGAGCCCCAGTTTAATG |
NGG |
2 |
0.1641 |
Tier II |
18 |
NC_000073.6 |
7 |
45684949 |
+ |
Sec1 |
NNNAGATCCCCAGTCCTATG |
NGG |
2 |
0.1558 |
Tier II |
19 |
NC_000073.6 |
7 |
45684949 |
+ |
Ntn5 |
NNNAGATCCCCAGTCCTATG |
NGG |
2 |
0.1558 |
Tier II |
20 |
NC_000072.6 |
6 |
8016149 |
+ |
Col28a1 |
NNNAGAGCCGCTGTTCTATG |
NGG |
2 |
0.1296 |
Tier II |
21 |
NC_000068.7 |
2 |
28718850 |
- |
Ak8 |
NNNAGAGCCCCAGCTCTAGG |
NGG |
2 |
0.0714 |
Tier II |
22 |
NC_000076.6 |
10 |
81230311 |
- |
Atcay |
NNNAGAGCCCCAGTTCTCGG |
NGG |
2 |
0.0476 |
Tier II |
23 |
NC_000071.6 |
5 |
74059684 |
+ |
Usp46 |
NNNAGAGCCCAAGTGCTATG |
NGG |
2 |
0.0375 |
Tier II |
24 |
NC_000071.6 |
5 |
142597907 |
+ |
Mmd2 |
NNNAGAGCCCCAGTGCTATG |
NAG |
2 |
0.013 |
Tier II |
25 |
NC_000068.7 |
2 |
60802284 |
+ |
Rbms1 |
NNNAAAGCCCCAGGTCTATG |
NGG |
2 |
0.0 |
Tier II |
26 |
NC_000075.6 |
9 |
72497389 |
+ |
Gm33004 |
NNNAGAGCCCAAGTTCTTTG |
NGG |
2 |
0.375 |
Tier III |
27 |
NC_000081.6 |
15 |
3374586 |
+ |
Gm41254 |
NNNAGAGCCCCAGTTTAATG |
NGG |
2 |
0.1641 |
Tier III |
28 |
NC_000072.6 |
6 |
81992196 |
- |
Gm20383 |
NNNGGAGCCCCAGTTCTATG |
NAG |
2 |
0.162 |
Tier III |
Other clones with same target sequence:
(none)