Construct: sgRNA BRDN0001146736
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGTCACTGACCTTTGTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PI4K2A (55361)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76821
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
97651136 |
- |
PI4K2A |
NNNTCACTGACCTTTGTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
4432201 |
- |
GLIS3 |
NNNACACTGACCTTTGTACG |
NGG |
1 |
0.4762 |
Tier II |
| 3 |
NC_000002.12 |
2 |
177740257 |
- |
PDE11A |
NNNTCACTGACCTTTGTATG |
NGG |
1 |
0.4615 |
Tier II |
| 4 |
NC_000012.12 |
12 |
133047362 |
- |
ZNF84 |
NNNTCAATGGCCTTTGTACG |
NGG |
2 |
0.4167 |
Tier II |
| 5 |
NC_000018.10 |
18 |
61894156 |
+ |
RNF152 |
NNNTCACTGACCTTTCTCCG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000012.12 |
12 |
9943460 |
+ |
LINC02470 |
NNNTCTCTGACGTTTGTACG |
NGG |
2 |
0.3175 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
42100832 |
- |
Pi4k2a |
NNNTCACTGACCTTTGTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000085.6 |
19 |
4142513 |
- |
Gpr152 |
NNNTCACTGAGCTCTGTACG |
NGG |
2 |
0.0714 |
Tier I |
| 3 |
NC_000076.6 |
10 |
128288853 |
- |
Stat2 |
NNNTCACTGACCTTTATACA |
NGG |
2 |
0.9375 |
Tier II |
| 4 |
NC_000067.6 |
1 |
157549921 |
+ |
Sec16b |
NNNTCCCTGACCTTTGTACA |
NGG |
2 |
0.4261 |
Tier II |
| 5 |
NC_000083.6 |
17 |
90251186 |
+ |
Nrxn1 |
NNNTCAGTGACCTTTGTAAG |
NGG |
2 |
0.2017 |
Tier II |
| 6 |
NC_000085.6 |
19 |
4142513 |
- |
Cabp4 |
NNNTCACTGAGCTCTGTACG |
NGG |
2 |
0.0714 |
Tier II |
| 7 |
NC_000068.7 |
2 |
179747295 |
- |
Cdh4 |
NNNTCACTGACTTTTGTAGG |
NGG |
2 |
0.0673 |
Tier II |
| 8 |
NC_000085.6 |
19 |
17010954 |
+ |
Prune2 |
NNNTCACTGACCTTGGTACT |
NGG |
2 |
0.035 |
Tier II |
| 9 |
NC_000072.6 |
6 |
146328212 |
- |
Itpr2 |
NNNTTACTGACCTTTGTACG |
NTG |
2 |
0.0248 |
Tier II |
| 10 |
NC_000074.6 |
8 |
93079756 |
- |
Ces1b |
NNNTCACTGACCCTTGTACG |
NGC |
2 |
0.0175 |
Tier II |
| 11 |
NC_000079.6 |
13 |
8334865 |
- |
Adarb2 |
NNNTCACTGACCTGTGTATG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
