Construct: sgRNA BRDN0001146737
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATCGAATGGAACAGCCTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ABL2 (27)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77179
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
179114919 |
- |
ABL2 |
NNNCGAATGGAACAGCCTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
119032628 |
- |
IGSF11 |
NNNCCAAAGGAACAGCCTGA |
NGG |
2 |
0.6286 |
Tier II |
| 3 |
NC_000001.11 |
1 |
77967327 |
+ |
FUBP1 |
NNNTGAATGGAACAGTCTGA |
NGG |
2 |
0.2462 |
Tier II |
| 4 |
NC_000012.12 |
12 |
111118564 |
- |
CUX2 |
NNNCGAATGGTACAGCTTGA |
NGG |
2 |
0.1436 |
Tier II |
| 5 |
NC_000007.14 |
7 |
130951051 |
+ |
LINC-PINT |
NNNAGACTGGAACAGCCTGA |
NGG |
2 |
0.3684 |
Tier III |
| 6 |
NC_000002.12 |
2 |
21353107 |
- |
LOC105374318 |
NNNCAAATGGAAGAGCCTGA |
NGG |
2 |
0.1182 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
156635177 |
+ |
Abl2 |
NNNCGAATGGAACAGCCTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000075.6 |
9 |
107526936 |
- |
Cacna2d2 |
NNNCGCATGGAACAGCCTGG |
NGG |
2 |
0.3476 |
Tier I |
| 3 |
NC_000077.6 |
11 |
46015871 |
+ |
Sox30 |
NNNCGGATGGAAGAGCCTGA |
NGG |
2 |
0.0974 |
Tier II |
| 4 |
NC_000085.6 |
19 |
4833572 |
+ |
Ccs |
NNNCTAATGGACCAGCCTGA |
NGG |
2 |
0.0789 |
Tier II |
| 5 |
NC_000080.6 |
14 |
66863735 |
- |
Dpysl2 |
NNNGGAATGGAACAGCCTGA |
NGA |
2 |
0.0347 |
Tier II |
| 6 |
NC_000080.6 |
14 |
53089690 |
- |
Tcra |
NNNCGAAGGGAACAGACTGA |
NGG |
2 |
0.7333 |
Tier III |
| 7 |
NC_000079.6 |
13 |
54392481 |
- |
LOC115488208 |
NNNAGAAAGGAACAGCCTGA |
NGG |
2 |
0.6737 |
Tier III |
| 8 |
NC_000084.6 |
18 |
5140376 |
+ |
LOC115486421 |
NNNCGCTTGGAACAGCCTGA |
NGG |
2 |
0.1989 |
Tier III |
| 9 |
NC_000080.6 |
14 |
98871318 |
+ |
Gm46478 |
NNNCTAATGGAACACCCTGA |
NGG |
2 |
0.0818 |
Tier III |
Other clones with same target sequence:
(none)
