Construct: sgRNA BRDN0001146749

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATGAAGTACTATATAGGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYRK1B (9149)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76622

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 39829880 - DYRK1B NNNGAAGTACTATATAGGTG NGG 0 1.0 Tier I
2 NC_000004.12 4 146404613 - SLC10A7 NNNGAAGCAATATATAGGTG NGG 2 0.8667 Tier II
3 NC_000005.10 5 43499671 + C5orf34 NNNAAAGTACTATATAGGTA NGG 2 0.8438 Tier II
4 NC_000003.12 3 44386253 - TCAIM NNNAAAGAACTATATAGGTG NGG 2 0.72 Tier II
5 NC_000014.9 14 81345125 + STON2 NNNGAAATACTATATAGGTT NGG 2 0.7 Tier II
6 NC_000015.10 15 43481147 - TP53BP1 NNNGAAGCACTATAAAGGTG NGG 2 0.5789 Tier II
7 NC_000002.12 2 204915016 - PARD3B NNNGAAGTCCTAAATAGGTG NGG 2 0.3956 Tier II
8 NC_000003.12 3 44249235 + TOPAZ1 NNNGAATTACTATATAGTTG NGG 2 0.381 Tier II
9 NC_000007.14 7 114335933 + FOXP2 NNNGAAGTACTATGTAGATG NGG 2 0.3231 Tier II
10 NC_000015.10 15 71666512 - THSD4 NNNGAAGTATTATATATGTG NGG 2 0.2353 Tier II
11 NC_000003.12 3 59788588 - FHIT NNNGAAGTACTGTATAGGAG NGG 2 0.2063 Tier II
12 NC_000002.12 2 36422333 + CRIM1 NNNGTAGTACTATATATGTG NGG 2 0.0909 Tier II
13 NC_000004.12 4 46058882 - GABRG1 NNNGAAGTACTATATTGGGG NGG 2 0.0 Tier II
14 NC_000005.10 5 43499671 + C5orf34-AS1 NNNAAAGTACTATATAGGTA NGG 2 0.8438 Tier III
15 NC_000009.12 9 42705234 + LOC124902164 NNNGAAATACTATATAGTTG NGG 2 0.6667 Tier III
16 NC_000016.10 16 60450083 + LOC101927605 NNNGAAATGCTATATAGGTG NGG 2 0.6667 Tier III
17 NC_000004.12 4 106006013 - LOC101929577 NNNGAAGTACTATATAAGTT NGG 2 0.6533 Tier III
18 NC_000008.11 8 116113869 - LINC00536 NNNCAAGTACTATATAGGTG NGG 1 0.5294 Tier III
19 NC_000001.11 1 30037301 + LINC01648 NNNGAAGTACTTTATAGATG NGG 2 0.2308 Tier III
20 NC_000003.12 3 59788588 - CFAP20DC-DT NNNGAAGTACTGTATAGGAG NGG 2 0.2063 Tier III
21 NC_000012.12 12 114103940 + LOC105369995 NNNGGAGTACTATATAGGTG NTG 2 0.0281 Tier III
22 NC_000018.10 18 24938957 + LINC01894 NNNTAAGTACTATATAGGTG NGT 2 0.0059 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 28183212 + Dyrk1b NNNGAAGTACTACATAGGTG NGG 1 0.7895 Tier I
2 NC_000082.6 16 94668953 + Dyrk1a NNNGAAGTACTACATAGGTA NGG 2 0.7401 Tier I
3 NC_000071.6 5 145195483 - Zkscan14 NNNGAAGTACTATAAATGTG NGG 2 0.1447 Tier I
4 NC_000080.6 14 13373085 - Synpr NNNGAAGTAATATATAGGTA NGG 2 0.8125 Tier II
5 NC_000071.6 5 108091620 + Mtf2 NNNAAAGTGCTATATAGGTG NGG 2 0.6 Tier II
6 NC_000074.6 8 14106700 + Dlgap2 NNNGCAGTCCTATATAGGTG NGG 2 0.2857 Tier II
7 NC_000068.7 2 168898437 + Zfp64 NNNGAAGTACTATATAAGGG NGG 2 0.2333 Tier II
8 NC_000080.6 14 59397273 + Gm45935 NNNGAAGTACAAGATAGGTG NGG 2 0.1957 Tier II
9 NC_000072.6 6 120219329 - B4galnt3 NNNGAAGTACTATACAGGTG NAG 2 0.0707 Tier II
10 NC_000086.7 X 92970139 + Gm38657 NNNGAAGTACTATCTAGGTC NGG 2 0.0918 Tier III
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Other clones with same target sequence:

(none)