Construct: sgRNA BRDN0001146752

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAGGGGTGATGGTACAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK5 (57144)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75713

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 9566231 + PAK5 NNNGGGGTGATGGTACAAGG NGG 0 1.0 Tier I
2 NC_000001.11 1 151287474 + ZNF687 NNNTGGGTGATGGTACAAGG NTG 2 0.0142 Tier I
3 NC_000001.11 1 3300737 - PRDM16 NNNGGGGGGATGGTACAAGG NGG 1 0.7333 Tier II
4 NC_000010.11 10 14219539 + FRMD4A NNNGAGGTCATGGTACAAGG NGG 2 0.4667 Tier II
5 NC_000012.12 12 54956810 - TESPA1 NNNGGGATGATGCTACAAGG NGG 2 0.4211 Tier II
6 NC_000007.14 7 39143770 + POU6F2 NNNGGGGTGAGGGTAAAAGG NGG 2 0.4 Tier II
7 NC_000011.10 11 113367043 - TTC12 NNNGGGGTGAGAGTACAAGG NGG 2 0.3733 Tier II
8 NC_000005.10 5 151565373 + FAT2 NNNGGGGTGATGGAACATGG NGG 2 0.3095 Tier II
9 NC_000018.10 18 75205368 - PTGR3 NNNGGGGTGGGGGTACAAGG NGG 2 0.2222 Tier II
10 NC_000002.12 2 70906459 - VAX2 NNNGGGGAGATGGTTCAAGG NGG 2 0.16 Tier II
11 NC_000007.14 7 6234044 - CYTH3 NNNGAGGTGATGGTACTAGG NGG 2 0.1156 Tier II
12 NC_000001.11 1 2786241 - TTC34 NNNGGGGTGATGGCACAGGG NGG 2 0.1143 Tier II
13 NC_000008.11 8 15838447 - TUSC3 NNNGGGGTAATGGTAGAAGG NGG 2 0.0989 Tier II
14 NC_000003.12 3 113446541 - SPICE1 NNNGGGGTGAGGGTAGAAGG NGG 2 0.0615 Tier II
15 NC_000003.12 3 171800937 - PLD1 NNNGGGGTGATGGTATTAGG NGG 2 0.041 Tier II
16 NC_000002.12 2 154128645 + GALNT13 NNNGGGGTGATGGTAGGAGG NGG 2 0.0271 Tier II
17 NC_000006.12 6 57419091 + PRIM2 NNNGTGGTGATGGTACAAGG NGA 2 0.0208 Tier II
18 NC_000019.10 19 11238381 + DOCK6 NNNGGGGTGATGGGACAAGG NTG 2 0.0 Tier II
19 NC_000020.11 20 9566231 + LOC105372523 NNNGGGGTGATGGTACAAGG NGG 0 1.0 Tier III
20 NC_000007.14 7 106776173 - LINC02577 NNNGGGGTGGTGGTAAAAGG NGG 2 0.5556 Tier III
21 NC_000001.11 1 56415366 - LINC01767 NNNGGGGTGATGGAACAAAG NGG 2 0.4422 Tier III
22 NC_000001.11 1 56415366 - LOC124904185 NNNGGGGTGATGGAACAAAG NGG 2 0.4422 Tier III
23 NC_000015.10 15 93760522 - LOC107983974 NNNGGGGTGAGGGAACAAGG NGG 2 0.2476 Tier III
24 NC_000001.11 1 107977608 - VAV3-AS1 NNNTGGGTGATGGTACATGG NGG 2 0.1818 Tier III
25 NC_000021.9 21 38786650 - LOC107985480 NNNGGGGTGATGGTGCAAGG NAG 2 0.1685 Tier III
26 NC_000003.12 3 113446541 - SPICE1-CFAP44 NNNGGGGTGAGGGTAGAAGG NGG 2 0.0615 Tier III
27 NC_000001.11 1 2037255 - LOC105378590 NNNGGGGTGATGGTATTAGG NGG 2 0.041 Tier III
28 NC_000006.12 6 60320604 + PRIM2BP NNNGTGGTGATGGTACAAGG NGA 2 0.0208 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 49450039 + Ttc12 NNNTGGATGATGGTACAAGG NGG 2 0.3636 Tier II
2 NC_000086.7 X 130152348 + Diaph2 NNNGGGGTCATGGTACAATG NGG 2 0.359 Tier II
3 NC_000071.6 5 142968527 - Fscn1 NNNGGGGTGCTGGTAAAAGG NGG 2 0.3333 Tier II
4 NC_000073.6 7 45225834 + Tead2 NNNTGGGTGATGGTACAAAG NGG 2 0.2597 Tier II
5 NC_000086.7 X 135720073 + Tmsb15a NNNGAGGTGATGGCACAAGG NGG 2 0.2476 Tier II
6 NC_000072.6 6 111448670 + Grm7 NNNGGGGTCAGGGTACAAGG NGG 2 0.2154 Tier II
7 NC_000070.6 4 140032359 - Igsf21 NNNGGCGTGATGGTACAAGG NTG 2 0.0266 Tier II
8 NC_000083.6 17 46388453 + Zfp318 NNNGGGGTGATGGTACAGGG NGT 2 0.0065 Tier II
9 NC_000072.6 6 72426318 + Ggcx NNNGGGGTGATGGTAGAAGG NGT 2 0.0025 Tier II
10 NC_000080.6 14 63233158 - Gata4 NNNGGGGTGATGGGAGAAGG NGG 2 0.0 Tier II
11 NC_000076.6 10 79853887 + E130317F20Rik NNNGGGGTGAAGGTACAAGA NGG 2 0.7031 Tier III
12 NC_000080.6 14 76748050 - LOC115485926 NNNGGAGTGATGCTACAAGG NGG 2 0.4211 Tier III
13 NC_000083.6 17 57266794 - Gm46575 NNNGGGGAGATGCTACAAGG NGG 2 0.3368 Tier III
14 NC_000074.6 8 99552857 - A330008L17Rik NNNGGGGTGATAGTAGAAGG NGG 2 0.1436 Tier III
15 NC_000070.6 4 125299426 + Gm46869 NNNGGGGTGATGGAAGAAGG NGG 2 0.0952 Tier III
16 NC_000071.6 5 140549202 - Gm42808 NNNGGTGTGATGGTACAAGG NGC 2 0.0148 Tier III
17 NC_000082.6 16 78061596 + Gm31431 NNNGGGGTGATGGGATAAGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)