Construct: sgRNA BRDN0001146754
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTGTTACAAAGCATTAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRRK2 (120892)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77330
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
40251345 |
+ |
LRRK2 |
NNNGTTACAAAGCATTAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
148771745 |
+ |
AFF2 |
NNNGTTACAAAGTATTAACA |
NGG |
2 |
0.6563 |
Tier II |
| 3 |
NC_000002.12 |
2 |
29715756 |
+ |
ALK |
NNNGTTACAAAGCATTAACA |
NTG |
2 |
0.0365 |
Tier II |
| 4 |
NC_000009.12 |
9 |
89974578 |
+ |
LOC101927847 |
NNNGTTACAAAATATTAACG |
NGG |
2 |
0.6533 |
Tier III |
| 5 |
NC_000004.12 |
4 |
23319298 |
- |
LOC105374523 |
NNNGTTACAAAGCACTAACG |
NGG |
1 |
0.2727 |
Tier III |
| 6 |
NC_000004.12 |
4 |
23319298 |
- |
LOC105374524 |
NNNGTTACAAAGCACTAACG |
NGG |
1 |
0.2727 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
96121355 |
+ |
Otud7b |
NNNGGTACAAAGCATAAACG |
NGG |
2 |
0.5818 |
Tier II |
| 2 |
NC_000079.6 |
13 |
32928245 |
+ |
Serpinb1c |
NNNGTGACAAAGCATTAACT |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000074.6 |
8 |
90249507 |
+ |
Tox3 |
NNNGTTACACAGCATTAACT |
NGG |
2 |
0.2333 |
Tier II |
| 4 |
NC_000083.6 |
17 |
90320242 |
+ |
Nrxn1 |
NNNGTTACAAAGCATTTAAG |
NGG |
2 |
0.0571 |
Tier II |
| 5 |
NC_000068.7 |
2 |
163920333 |
+ |
Gm35886 |
NNNGTTACAAAGTATTAGCG |
NGG |
2 |
0.28 |
Tier III |
| 6 |
NC_000067.6 |
1 |
137940542 |
- |
Gm4258 |
NNNGTTACAAAGCATCAACG |
NTG |
2 |
0.026 |
Tier III |
Other clones with same target sequence:
(none)
