Construct: sgRNA BRDN0001146755

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGGGTAGCAGACAAACCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRAF (673)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76481

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 140783146 + BRAF NNNGGTAGCAGACAAACCTG NGG 0 1.0 Tier I
2 NC_000023.11 X 81210645 - SH3BGRL NNNGGAATCAGACAAACCTG NGG 2 0.5417 Tier II
3 NC_000001.11 1 33741739 - CSMD2 NNNGGCAGCAGACTAACCTG NGG 2 0.4848 Tier II
4 NC_000005.10 5 179224808 - ADAMTS2 NNNGGTGGCAGACAGACCTG NGG 2 0.4588 Tier II
5 NC_000005.10 5 179224857 - ADAMTS2 NNNGGTGGCAGACAGACCTG NGG 2 0.4588 Tier II
6 NC_000019.10 19 1636909 + TCF3 NNNGCGAGCAGACAAACCTG NGG 2 0.449 Tier II
7 NC_000008.11 8 23309351 + LOXL2 NNNGGCAGCACACAAACCTG NGG 2 0.3896 Tier II
8 NC_000003.12 3 126953185 - CHCHD6 NNNGGGATCAGACAAACCTG NGG 2 0.3571 Tier II
9 NC_000022.11 22 44000110 - PARVB NNNGGTAGAAGAAAAACCTG NGG 2 0.3297 Tier II
10 NC_000016.10 16 78627232 - WWOX NNNGGTAGCAAACACACCTG NGG 2 0.2727 Tier II
11 NC_000005.10 5 169595211 + SPDL1 NNNGGTAGCAGAAAAACATG NGG 2 0.2071 Tier II
12 NC_000017.11 17 46795192 - WNT3 NNNGGGAGCAGACAAGCCTG NGG 2 0.1099 Tier II
13 NC_000017.11 17 46795192 - LRRC37A2 NNNGGGAGCAGACAAGCCTG NGG 2 0.1099 Tier II
14 NC_000016.10 16 89143745 - ACSF3 NNNGGTAGCAGAAAAACCAG NGG 2 0.1099 Tier II
15 NC_000008.11 8 51518854 + PXDNL NNNGGTAGCAGAAAAACCTG NGA 2 0.0267 Tier II
16 NC_000015.10 15 83286420 + LOC105370932 NNNGGTAGCAGATAAACCTC NGG 2 0.3 Tier III
17 NC_000023.11 X 75583991 - BRAFP1 NNNTGTGGCAGACAAACCTG NGG 2 0.2567 Tier III
18 NC_000023.11 X 75583991 - LOC107985664 NNNTGTGGCAGACAAACCTG NGG 2 0.2567 Tier III
19 NC_000007.14 7 65311338 + LOC105375334 NNNGGTAGCAGTTAAACCTG NGG 2 0.2333 Tier III
20 NC_000014.9 14 49867910 + LOC105378179 NNNGGTACCAGACAAAGCTG NGG 2 0.0362 Tier III
21 NC_000001.11 1 59038915 - LINC01358 NNNGGGAGCAGACAAAGCTG NGG 2 0.0336 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 121915624 + Cyp8b1 NNNGGGAGTAGACAAACCTG NGG 2 0.5 Tier I
2 NC_000071.6 5 125427530 + Dhx37 NNNGGTAGGAGACAACCCTG NGG 2 0.0 Tier I
3 NC_000075.6 9 122243157 + Ano10 NNNGGAAGCAGACAGACCTG NGG 2 0.5633 Tier II
4 NC_000081.6 15 64335780 + Asap1 NNNGCTAGCAGACAAACCTC NGG 2 0.3367 Tier II
5 NC_000070.6 4 21768752 + Usp45 NNNGGTACCAGACAAACATG NGG 2 0.3314 Tier II
6 NC_000077.6 11 109391566 - Gna13 NNNTGTGGCAGACAAACCTG NGG 2 0.2567 Tier II
7 NC_000072.6 6 126164195 - Ntf3 NNNGGTAGCAGACAAAAATG NGG 2 0.2513 Tier II
8 NC_000075.6 9 120149988 - Mobp NNNTGGAGCAGACAAACCTG NGG 2 0.2078 Tier II
9 NC_000075.6 9 21660033 + Smarca4 NNNGGTACCAGACACACCTG NGG 2 0.1678 Tier II
10 NC_000081.6 15 73224824 - Ptk2 NNNGGAAGCAGACAAGCCTG NGG 2 0.1667 Tier II
11 NC_000077.6 11 68471346 - Pik3r5 NNNGGTAGCATAAAAACCTG NGG 2 0.1479 Tier II
12 NC_000068.7 2 10546081 - Sfmbt2 NNNGCTAGCAGACAAAGCTG NGG 2 0.0462 Tier II
13 NC_000074.6 8 106162239 + Pla2g15 NNNGGTAGCAGGCAAAGCTG NGG 2 0.0425 Tier II
14 NC_000075.6 9 117300497 - Rbms3 NNNGGTAGCAGACACAGCTG NGG 2 0.016 Tier II
15 NC_000067.6 1 103066950 + Gm18728 NNNGGAAACAGACAAACCTG NGG 2 0.8667 Tier III
16 NC_000085.6 19 59690973 - Gm41878 NNNGGTATCAGACAAATCTG NGG 2 0.2917 Tier III
17 NC_000085.6 19 59690973 - Gm46661 NNNGGTATCAGACAAATCTG NGG 2 0.2917 Tier III
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Other clones with same target sequence:

(none)