Construct: sgRNA BRDN0001146755
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGGTAGCAGACAAACCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BRAF (673)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76481
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000007.14 |
7 |
140783146 |
+ |
BRAF |
NNNGGTAGCAGACAAACCTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000023.11 |
X |
81210645 |
- |
SH3BGRL |
NNNGGAATCAGACAAACCTG |
NGG |
2 |
0.5417 |
Tier II |
3 |
NC_000001.11 |
1 |
33741739 |
- |
CSMD2 |
NNNGGCAGCAGACTAACCTG |
NGG |
2 |
0.4848 |
Tier II |
4 |
NC_000005.10 |
5 |
179224808 |
- |
ADAMTS2 |
NNNGGTGGCAGACAGACCTG |
NGG |
2 |
0.4588 |
Tier II |
5 |
NC_000005.10 |
5 |
179224857 |
- |
ADAMTS2 |
NNNGGTGGCAGACAGACCTG |
NGG |
2 |
0.4588 |
Tier II |
6 |
NC_000019.10 |
19 |
1636909 |
+ |
TCF3 |
NNNGCGAGCAGACAAACCTG |
NGG |
2 |
0.449 |
Tier II |
7 |
NC_000008.11 |
8 |
23309351 |
+ |
LOXL2 |
NNNGGCAGCACACAAACCTG |
NGG |
2 |
0.3896 |
Tier II |
8 |
NC_000003.12 |
3 |
126953185 |
- |
CHCHD6 |
NNNGGGATCAGACAAACCTG |
NGG |
2 |
0.3571 |
Tier II |
9 |
NC_000022.11 |
22 |
44000110 |
- |
PARVB |
NNNGGTAGAAGAAAAACCTG |
NGG |
2 |
0.3297 |
Tier II |
10 |
NC_000016.10 |
16 |
78627232 |
- |
WWOX |
NNNGGTAGCAAACACACCTG |
NGG |
2 |
0.2727 |
Tier II |
11 |
NC_000005.10 |
5 |
169595211 |
+ |
SPDL1 |
NNNGGTAGCAGAAAAACATG |
NGG |
2 |
0.2071 |
Tier II |
12 |
NC_000017.11 |
17 |
46795192 |
- |
WNT3 |
NNNGGGAGCAGACAAGCCTG |
NGG |
2 |
0.1099 |
Tier II |
13 |
NC_000017.11 |
17 |
46795192 |
- |
LRRC37A2 |
NNNGGGAGCAGACAAGCCTG |
NGG |
2 |
0.1099 |
Tier II |
14 |
NC_000016.10 |
16 |
89143745 |
- |
ACSF3 |
NNNGGTAGCAGAAAAACCAG |
NGG |
2 |
0.1099 |
Tier II |
15 |
NC_000008.11 |
8 |
51518854 |
+ |
PXDNL |
NNNGGTAGCAGAAAAACCTG |
NGA |
2 |
0.0267 |
Tier II |
16 |
NC_000015.10 |
15 |
83286420 |
+ |
LOC105370932 |
NNNGGTAGCAGATAAACCTC |
NGG |
2 |
0.3 |
Tier III |
17 |
NC_000023.11 |
X |
75583991 |
- |
BRAFP1 |
NNNTGTGGCAGACAAACCTG |
NGG |
2 |
0.2567 |
Tier III |
18 |
NC_000023.11 |
X |
75583991 |
- |
LOC107985664 |
NNNTGTGGCAGACAAACCTG |
NGG |
2 |
0.2567 |
Tier III |
19 |
NC_000007.14 |
7 |
65311338 |
+ |
LOC105375334 |
NNNGGTAGCAGTTAAACCTG |
NGG |
2 |
0.2333 |
Tier III |
20 |
NC_000014.9 |
14 |
49867910 |
+ |
LOC105378179 |
NNNGGTACCAGACAAAGCTG |
NGG |
2 |
0.0362 |
Tier III |
21 |
NC_000001.11 |
1 |
59038915 |
- |
LINC01358 |
NNNGGGAGCAGACAAAGCTG |
NGG |
2 |
0.0336 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000075.6 |
9 |
121915624 |
+ |
Cyp8b1 |
NNNGGGAGTAGACAAACCTG |
NGG |
2 |
0.5 |
Tier I |
2 |
NC_000071.6 |
5 |
125427530 |
+ |
Dhx37 |
NNNGGTAGGAGACAACCCTG |
NGG |
2 |
0.0 |
Tier I |
3 |
NC_000075.6 |
9 |
122243157 |
+ |
Ano10 |
NNNGGAAGCAGACAGACCTG |
NGG |
2 |
0.5633 |
Tier II |
4 |
NC_000081.6 |
15 |
64335780 |
+ |
Asap1 |
NNNGCTAGCAGACAAACCTC |
NGG |
2 |
0.3367 |
Tier II |
5 |
NC_000070.6 |
4 |
21768752 |
+ |
Usp45 |
NNNGGTACCAGACAAACATG |
NGG |
2 |
0.3314 |
Tier II |
6 |
NC_000077.6 |
11 |
109391566 |
- |
Gna13 |
NNNTGTGGCAGACAAACCTG |
NGG |
2 |
0.2567 |
Tier II |
7 |
NC_000072.6 |
6 |
126164195 |
- |
Ntf3 |
NNNGGTAGCAGACAAAAATG |
NGG |
2 |
0.2513 |
Tier II |
8 |
NC_000075.6 |
9 |
120149988 |
- |
Mobp |
NNNTGGAGCAGACAAACCTG |
NGG |
2 |
0.2078 |
Tier II |
9 |
NC_000075.6 |
9 |
21660033 |
+ |
Smarca4 |
NNNGGTACCAGACACACCTG |
NGG |
2 |
0.1678 |
Tier II |
10 |
NC_000081.6 |
15 |
73224824 |
- |
Ptk2 |
NNNGGAAGCAGACAAGCCTG |
NGG |
2 |
0.1667 |
Tier II |
11 |
NC_000077.6 |
11 |
68471346 |
- |
Pik3r5 |
NNNGGTAGCATAAAAACCTG |
NGG |
2 |
0.1479 |
Tier II |
12 |
NC_000068.7 |
2 |
10546081 |
- |
Sfmbt2 |
NNNGCTAGCAGACAAAGCTG |
NGG |
2 |
0.0462 |
Tier II |
13 |
NC_000074.6 |
8 |
106162239 |
+ |
Pla2g15 |
NNNGGTAGCAGGCAAAGCTG |
NGG |
2 |
0.0425 |
Tier II |
14 |
NC_000075.6 |
9 |
117300497 |
- |
Rbms3 |
NNNGGTAGCAGACACAGCTG |
NGG |
2 |
0.016 |
Tier II |
15 |
NC_000067.6 |
1 |
103066950 |
+ |
Gm18728 |
NNNGGAAACAGACAAACCTG |
NGG |
2 |
0.8667 |
Tier III |
16 |
NC_000085.6 |
19 |
59690973 |
- |
Gm41878 |
NNNGGTATCAGACAAATCTG |
NGG |
2 |
0.2917 |
Tier III |
17 |
NC_000085.6 |
19 |
59690973 |
- |
Gm46661 |
NNNGGTATCAGACAAATCTG |
NGG |
2 |
0.2917 |
Tier III |
Other clones with same target sequence:
(none)