Construct: sgRNA BRDN0001146757
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATCACCGAACGATGAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK11B (984)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77064
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
1642485 |
- |
CDK11B |
NNNTCACCGAACGATGAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
1709617 |
- |
CDK11A |
NNNTCACCGAACGATGAGAG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000011.10 |
11 |
35469878 |
+ |
PAMR1 |
NNNTCTCAGAACGATGAGAG |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000003.12 |
3 |
138269691 |
+ |
ARMC8 |
NNNTCAGCGAAAGATGAGAG |
NGG |
2 |
0.3361 |
Tier II |
| 5 |
NC_000003.12 |
3 |
138269691 |
+ |
NME9 |
NNNTCAGCGAAAGATGAGAG |
NGG |
2 |
0.3361 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
157069684 |
+ |
Negr1 |
NNNTCACTGAAAGATGAGAG |
NGG |
2 |
0.625 |
Tier II |
| 2 |
NC_000067.6 |
1 |
175322965 |
- |
Rgs7 |
NNNTCACTGAAGGATGAGAG |
NGG |
2 |
0.3889 |
Tier II |
| 3 |
NC_000068.7 |
2 |
72066141 |
- |
Rapgef4 |
NNNTCACAGAATGATGAGAG |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000082.6 |
16 |
45561521 |
- |
Slc9c1 |
NNNTCACCTAACTATGAGAG |
NGG |
2 |
0.16 |
Tier II |
Other clones with same target sequence:
(none)
