Construct: sgRNA BRDN0001146760

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGCTTCGCAGCGGTACCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAGK (55577)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76325

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 71070822 - NAGK NNNCTTCGCAGCGGTACCAG NGG 0 1.0 Tier I
2 NC_000021.9 21 43812524 - AATBC NNNCTGGGCAGCGGTACCAG NGG 2 0.2689 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 78270001 - Agpat3 NNNCTTCGCAGAGGTACCAG NGA 2 0.0496 Tier II
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Other clones with same target sequence:

(none)