Construct: sgRNA BRDN0001146762
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATCCCGGTGTACAATGCCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK1 (8536)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77478
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
9762936 |
+ |
CAMK1 |
NNNCCCGGTGTACAATGCCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
45915618 |
- |
EPG5 |
NNNCCCGGTGTAGAATGCCT |
NGG |
2 |
0.0409 |
Tier I |
| 3 |
NC_000014.9 |
14 |
23405319 |
- |
MYH6 |
NNNGCCGGTGTACAATGCCG |
NGG |
2 |
0.0294 |
Tier I |
| 4 |
NC_000003.12 |
3 |
9762936 |
+ |
OGG1 |
NNNCCCGGTGTACAATGCCC |
NGG |
0 |
1.0 |
Tier II |
| 5 |
NC_000012.12 |
12 |
1632545 |
- |
WNT5B |
NNNCCCTGTGTACAAGGCCC |
NGG |
2 |
0.1978 |
Tier II |
| 6 |
NC_000003.12 |
3 |
122695057 |
- |
PARP14 |
NNNCCAGGTGTACAATGCCC |
NTG |
2 |
0.0362 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
113339307 |
+ |
Camk1 |
NNNCCCGGTGCACAATGCCC |
NGG |
1 |
0.6667 |
Tier I |
| 2 |
NC_000086.7 |
X |
73658041 |
+ |
Pnck |
NNNCCCGGTGCACGATGCCC |
NGG |
2 |
0.3111 |
Tier I |
| 3 |
NC_000070.6 |
4 |
89138479 |
- |
Mtap |
NNNCCCAGTATACAATGCCC |
NGG |
2 |
0.9333 |
Tier II |
| 4 |
NC_000083.6 |
17 |
83846310 |
- |
Haao |
NNNCCTGGTGTTCAATGCCC |
NGG |
2 |
0.3095 |
Tier II |
| 5 |
NC_000083.6 |
17 |
6604769 |
- |
Dynlt1c |
NNNCACGGTGTACAATTCCC |
NGG |
2 |
0.1429 |
Tier II |
| 6 |
NC_000083.6 |
17 |
6652072 |
+ |
Dynlt1f |
NNNCACGGTGTACAATTCCC |
NGG |
2 |
0.1429 |
Tier II |
| 7 |
NC_000078.6 |
12 |
29227440 |
- |
Gm31569 |
NNNCCCGGTCTACAAAGCCC |
NGG |
2 |
0.3636 |
Tier III |
Other clones with same target sequence:
(none)
