Construct: sgRNA BRDN0001146763

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGTCCTGGGAACTCCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSK3B (2932)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77283

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 119876494 - GSK3B NNNTCCTGGGAACTCCAACA NGG 0 1.0 Tier I
2 NC_000014.9 14 64741877 - PLEKHG3 NNNTCCTGGGAGCTCAAACA NGG 2 0.7222 Tier I
3 NC_000003.12 3 10558237 - ATP2B2 NNNTCCTGGGAGCACCAACA NGG 2 0.4471 Tier II
4 NC_000011.10 11 19670330 - NAV2 NNNTCCTAGGAAATCCAACA NGG 2 0.3846 Tier II
5 NC_000011.10 11 43379027 - TTC17 NNNTCCTGTGAACTCCAACT NGG 2 0.32 Tier II
6 NC_000012.12 12 109817529 + TRPV4 NNNTCCTGGGGACTCCAAAA NGG 2 0.2786 Tier II
7 NC_000003.12 3 189685560 + TP63 NNNTCCTGTGAACTCCATCA NGG 2 0.2667 Tier II
8 NC_000002.12 2 47441261 - MSH2 NNNTACTGGGAACTCCAAAA NGG 2 0.2449 Tier II
9 NC_000017.11 17 56177012 - ANKFN1 NNNTCCTGGAAACTCCAACC NGG 2 0.2121 Tier II
10 NC_000018.10 18 24205317 - OSBPL1A NNNTCATGGGAACTCCACCA NGG 2 0.1769 Tier II
11 NC_000013.11 13 112561707 - TUBGCP3 NNNTCCTGGAAACTCCCACA NGG 2 0.1647 Tier II
12 NC_000002.12 2 134540320 + TMEM163 NNNTCCTGGGAACACCAACC NGG 2 0.1407 Tier II
13 NC_000023.11 X 46536830 + ZNF674 NNNTCCTGGGAACCCCAAAA NGG 2 0.1224 Tier II
14 NC_000017.11 17 36614569 + MRM1 NNNTCCTGGGCACCCCAACA NGG 2 0.1143 Tier II
15 NC_000014.9 14 49789095 + NEMF NNNTCCTGGGAACTCCAGGA NGG 2 0.05 Tier II
16 NC_000005.10 5 170491208 + KCNIP1 NNNTCCTGGAAACTGCAACA NGG 2 0.0467 Tier II
17 NC_000017.11 17 32501870 + MYO1D NNNTCCTGGGAACTCCAACT NGA 2 0.0417 Tier II
18 NC_000010.11 10 123910467 - CPXM2 NNNCCCTGGGAACTGCAACA NGG 2 0.0324 Tier II
19 NC_000003.12 3 57144868 - IL17RD NNNTCCTGGGAACCCCAACA NGA 2 0.0198 Tier II
20 NC_000015.10 15 95157966 - LOC105370991 NNNTCATGTGAACTCCAACA NGG 2 0.4952 Tier III
21 NC_000016.10 16 52307266 + LOC107984901 NNNTCATGGGAAGTCCAACA NGG 2 0.1266 Tier III
22 NC_000008.11 8 127932069 + PVT1 NNNTCCTGGGAACACCACCA NGG 2 0.1179 Tier III
23 NC_000004.12 4 107587337 + LOC105377358 NNNTCCTGGCAACCCCAACA NGG 2 0.1143 Tier III
24 NC_000006.12 6 100898595 - LOC107984041 NNNTCCTGGCAACTGCAACA NGG 2 0.02 Tier III
25 NC_000004.12 4 22792796 + GBA3 NNNTCCTGGGAACTCCCACA NTG 2 0.0069 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 38208010 + Gsk3b NNNTCCTAGGAACACCAACA NGG 2 0.619 Tier I
2 NC_000070.6 4 154927137 + Tnfrsf14 NNNTCCTGGGAACTCCCACC NGG 2 0.0401 Tier I
3 NC_000068.7 2 119947161 - Mga NNNTCCTGGGAGCTGCAACA NGG 2 0.0361 Tier I
4 NC_000076.6 10 60657351 + Cdh23 NNNTCCTGGGAACACAAACA NGG 2 0.619 Tier II
5 NC_000067.6 1 165324599 - Gpr161 NNNTCCTGTGAACTCCAACA NGG 1 0.5333 Tier II
6 NC_000075.6 9 97012804 - Spsb4 NNNTCCTGGGAACTCAAGCA NGG 2 0.4 Tier II
7 NC_000074.6 8 108629579 + Zfhx3 NNNGCCTGGGAACTCCAGCA NGG 2 0.25 Tier II
8 NC_000082.6 16 13111041 - Ercc4 NNNTCCTGGGGAATCCAACA NGG 2 0.25 Tier II
9 NC_000069.6 3 28084345 + Pld1 NNNTGCTGGGAACTCCAGCA NGG 2 0.24 Tier II
10 NC_000068.7 2 7173814 - Celf2 NNNTCCAGGGAACTCCAACA NAG 2 0.2269 Tier II
11 NC_000071.6 5 71600738 + Gabra4 NNNTCCTGGGAACTCAACCA NGG 2 0.1905 Tier II
12 NC_000068.7 2 175276738 + Gm14440 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
13 NC_000068.7 2 175374112 + Zfp968 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
14 NC_000068.7 2 175471663 + Gm4724 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
15 NC_000068.7 2 175471663 + Zfp965 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
16 NC_000068.7 2 175604500 - Gm4724 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
17 NC_000068.7 2 175702049 - Gm4724 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
18 NC_000068.7 2 175799425 - Gm14288 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
19 NC_000068.7 2 175929451 - Zfp966 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
20 NC_000068.7 2 176522654 + Zfp967 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
21 NC_000068.7 2 176672200 + Zfp973 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
22 NC_000068.7 2 177466384 + Zfp970 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
23 NC_000068.7 2 178024921 + Zfp971 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier II
24 NC_000080.6 14 73281153 - Rb1 NNNTCCTGGGTACTCCAACA NAG 2 0.0798 Tier II
25 NC_000080.6 14 10425260 + Fhit NNNTACTGGGAACTCCTACA NGG 2 0.0762 Tier II
26 NC_000075.6 9 63339559 + Map2k5 NNNTGCTGGGAACTCCAAGA NGG 2 0.075 Tier II
27 NC_000083.6 17 46659669 + Cul7 NNNTCCTGGGGACTCCAACA NGA 2 0.0451 Tier II
28 NC_000073.6 7 138288193 - Tcerg1l NNNTCCTGGGAACTTCAACT NGG 2 0.04 Tier II
29 NC_000082.6 16 87366644 - N6amt1 NNNTCCTGGGAACTCCAACA NTG 1 0.039 Tier II
30 NC_000070.6 4 6794330 + Tox NNNTCCTGGGGACTGCAACA NGG 2 0.0325 Tier II
31 NC_000073.6 7 24690117 + Gm1096 NNNTCCTGGGGACTGCAACA NGG 2 0.0325 Tier II
32 NC_000073.6 7 24691846 + Gm1096 NNNTCCTGGGGACTGCAACA NGG 2 0.0325 Tier II
33 NC_000073.6 7 24711197 + Lypd10 NNNTCCTGGGGACTGCAACA NGG 2 0.0325 Tier II
34 NC_000073.6 7 24725003 - Lypd11 NNNTCCTGGGGACTGCAACA NGG 2 0.0325 Tier II
35 NC_000073.6 7 24748015 - Cd177 NNNTCCTGGGGACTGCAACA NGG 2 0.0325 Tier II
36 NC_000067.6 1 57839541 + Spats2l NNNGCCTGGGAACTGCAACA NGG 2 0.0313 Tier II
37 NC_000074.6 8 35620022 + Mfhas1 NNNTCCTGGGAACTCCAGCA NGA 2 0.0278 Tier II
38 NC_000070.6 4 65506214 - Astn2 NNNTACTGGGAACTCCAACA NGC 2 0.0127 Tier II
39 NC_000071.6 5 121360254 + Hectd4 NNNTCCTGGGAACTCCGACA NGC 2 0.0039 Tier II
40 NC_000074.6 8 48240607 - Tenm3 NNNTCCTGGGAACTCCGACA NGT 2 0.0028 Tier II
41 NC_000071.6 5 43698954 - Cc2d2a NNNTCCTGGGAAAGCCAACA NGG 2 0.0 Tier II
42 NC_000071.6 5 39976333 - Gm35191 NNNTGCAGGGAACTCCAACA NGG 2 0.525 Tier III
43 NC_000068.7 2 35554400 - Gm13446 NNNCCCTGGGAACTCCAGCA NGG 2 0.2588 Tier III
44 NC_000067.6 1 106159048 + Gm20753 NNNTCCTGGGAAATCCAACT NGG 2 0.2308 Tier III
45 NC_000082.6 16 90592614 - Gm36063 NNNTCCTGGGATCACCAACA NGG 2 0.2063 Tier III
46 NC_000071.6 5 138894485 + Gm31891 NNNCCCTGGGAACCCCAACA NGG 2 0.1849 Tier III
47 NC_000083.6 17 36532466 - Gm18809 NNNTCCTGGGATCTCCATCA NGG 2 0.1667 Tier III
48 NC_000068.7 2 176425269 + Zfp968-ps NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier III
49 NC_000068.7 2 177898790 + Gm14327 NNNTCCTGGGAACTCCAACT NAG 2 0.1556 Tier III
50 NC_000077.6 11 117038151 - Gm11728 NNNCCCTGGGAACTCCAACA NGC 2 0.0144 Tier III
51 NC_000073.6 7 85532010 - Gm18360 NNNTCCTGGGAACTCTAACA NGC 2 0.0068 Tier III
Download CSV

Other clones with same target sequence:

(none)