Construct: sgRNA BRDN0001146766
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTCCAAATAGTCAACGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NOL9 (79707)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76979
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
6541918 |
+ |
NOL9 |
NNNTCCAAATAGTCAACGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
124112282 |
- |
CNTNAP5 |
NNNTCCAAATAGTCAACTCA |
NGG |
1 |
0.6667 |
Tier II |
| 3 |
NC_000012.12 |
12 |
22540140 |
- |
C2CD5 |
NNNTCAAAATAGTCAACTCA |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000005.10 |
5 |
131599848 |
+ |
RAPGEF6 |
NNNTCCAAATGGCCAACGCA |
NGG |
2 |
0.5132 |
Tier II |
| 5 |
NC_000019.10 |
19 |
19591020 |
- |
PBX4 |
NNNTCCAGATACTCAACGCA |
NGG |
2 |
0.3882 |
Tier II |
| 6 |
NC_000011.10 |
11 |
78224960 |
+ |
GAB2 |
NNNTCCAAAGACTCAACGCA |
NGG |
2 |
0.2647 |
Tier II |
| 7 |
NC_000011.10 |
11 |
78224960 |
+ |
USP35 |
NNNTCCAAAGACTCAACGCA |
NGG |
2 |
0.2647 |
Tier II |
| 8 |
NC_000004.12 |
4 |
15534102 |
- |
CC2D2A |
NNNTCCAAATAGTCAATGCA |
NGT |
2 |
0.0075 |
Tier II |
| 9 |
NC_000012.12 |
12 |
49665439 |
+ |
FMNL3 |
NNNTCCAAATAGTCACCGAA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000002.12 |
2 |
241919763 |
+ |
LINC01237 |
NNNTCCAAATAGTCAAAGCA |
NGC |
2 |
0.0104 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
98397437 |
- |
Nmnat3 |
NNNTCCAAAGAGTCAACTCA |
NGG |
2 |
0.3333 |
Tier II |
Other clones with same target sequence:
(none)
