Construct: sgRNA BRDN0001146767
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAAGATGTGAGAAATCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- OBSCN (84033)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76116
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
228303711 |
+ |
OBSCN |
NNNAGATGTGAGAAATCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
13409598 |
- |
RAB28 |
NNNAGATGTGAGAAATCATG |
NGG |
1 |
0.4615 |
Tier II |
| 3 |
NC_000016.10 |
16 |
20995774 |
+ |
DNAH3 |
NNNAGTTTTGAGAAATCACG |
NGG |
2 |
0.4464 |
Tier II |
| 4 |
NC_000006.12 |
6 |
45926104 |
+ |
CLIC5 |
NNNAGGTCTGAGAAATCACG |
NGG |
2 |
0.4396 |
Tier II |
| 5 |
NC_000006.12 |
6 |
4947087 |
- |
CDYL |
NNNAGCTGTGAGAAAACACG |
NGG |
2 |
0.4132 |
Tier II |
| 6 |
NC_000006.12 |
6 |
157427594 |
+ |
ZDHHC14 |
NNNTGATGTGATAAATCACG |
NGG |
2 |
0.2448 |
Tier II |
| 7 |
NC_000004.12 |
4 |
47733690 |
- |
CORIN |
NNNAGATGTGAGAACTCAGG |
NGG |
2 |
0.0341 |
Tier II |
| 8 |
NC_000012.12 |
12 |
9514417 |
- |
LOC408186 |
NNNAAATGTGAGAAATCATG |
NGG |
2 |
0.4 |
Tier III |
| 9 |
NC_000012.12 |
12 |
31176218 |
+ |
OVOS2 |
NNNAAATGTGAGAAATCATG |
NGG |
2 |
0.4 |
Tier III |
| 10 |
NC_000008.11 |
8 |
9409047 |
- |
LOC105379231 |
NNNAGACGTGAGAAATAACG |
NGG |
2 |
0.3208 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
87910693 |
- |
BC037156 |
NNNAGATGTGAGTACTCACG |
NGG |
2 |
0.0818 |
Tier I |
| 2 |
NC_000079.6 |
13 |
114060310 |
- |
Arl15 |
NNNAGATGTGAAAAATCACA |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000085.6 |
19 |
34811743 |
+ |
Pank1 |
NNNAGAAGAGAGAAATCACG |
NGG |
2 |
0.8125 |
Tier II |
| 4 |
NC_000076.6 |
10 |
106882615 |
- |
Ppfia2 |
NNNAGATGTGAGAAATCTCA |
NGG |
2 |
0.4688 |
Tier II |
| 5 |
NC_000074.6 |
8 |
110870853 |
- |
Cog4 |
NNNGGATGTGAGAAATCACT |
NGG |
2 |
0.4375 |
Tier II |
| 6 |
NC_000076.6 |
10 |
85940497 |
+ |
Rtcb |
NNNAGATGTGAAAAATCATG |
NGG |
2 |
0.4308 |
Tier II |
| 7 |
NC_000071.6 |
5 |
113843691 |
- |
Coro1c |
NNNAGACTTGAGAAATCACG |
NGG |
2 |
0.4297 |
Tier II |
| 8 |
NC_000083.6 |
17 |
14282298 |
+ |
Smoc2 |
NNNAGATGAGAGAAATCATG |
NGG |
2 |
0.4286 |
Tier II |
| 9 |
NC_000071.6 |
5 |
149449583 |
+ |
Tex26 |
NNNAGATGCCAGAAATCACG |
NGG |
2 |
0.3692 |
Tier II |
| 10 |
NC_000072.6 |
6 |
119996693 |
- |
Wnk1 |
NNNAGATGTGAGAAACCACC |
NGG |
2 |
0.2857 |
Tier II |
| 11 |
NC_000077.6 |
11 |
5634933 |
- |
Ankrd36 |
NNNAGATGTCAGAAATCACT |
NGG |
2 |
0.28 |
Tier II |
| 12 |
NC_000073.6 |
7 |
46531520 |
+ |
Sergef |
NNNACATGTGAGAAAGCACG |
NGG |
2 |
0.272 |
Tier II |
| 13 |
NC_000078.6 |
12 |
91759830 |
+ |
Ston2 |
NNNAGATCTGAGAAATCAAG |
NGG |
2 |
0.2637 |
Tier II |
| 14 |
NC_000071.6 |
5 |
148175507 |
- |
Mtus2 |
NNNAGATGTGAGTAAGCACG |
NGG |
2 |
0.1038 |
Tier II |
| 15 |
NC_000078.6 |
12 |
101874237 |
- |
Trip11 |
NNNAGATGTGAGCAATCATG |
NGG |
2 |
0.0972 |
Tier II |
| 16 |
NC_000072.6 |
6 |
121036603 |
+ |
Mical3 |
NNNAGATGTGAGAATTCACG |
NGA |
2 |
0.0139 |
Tier II |
| 17 |
NC_000086.7 |
X |
73517091 |
+ |
Atp2b3 |
NNNAGATGTGAGAAATCAAG |
NGT |
2 |
0.0069 |
Tier II |
| 18 |
NC_000068.7 |
2 |
67393321 |
- |
Gm13599 |
NNNAGATGGGAGAAATCACA |
NGG |
2 |
0.5804 |
Tier III |
| 19 |
NC_000071.6 |
5 |
113843691 |
- |
Gm52824 |
NNNAGACTTGAGAAATCACG |
NGG |
2 |
0.4297 |
Tier III |
| 20 |
NC_000074.6 |
8 |
109170066 |
+ |
D030068K23Rik |
NNNAAATGTGATAAATCACG |
NGG |
2 |
0.3333 |
Tier III |
| 21 |
NC_000074.6 |
8 |
109170066 |
+ |
4922502B01Rik |
NNNAAATGTGATAAATCACG |
NGG |
2 |
0.3333 |
Tier III |
| 22 |
NC_000071.6 |
5 |
87910693 |
- |
1700066N21Rik |
NNNAGATGTGAGTACTCACG |
NGG |
2 |
0.0818 |
Tier III |
Other clones with same target sequence:
(none)
