Construct: sgRNA BRDN0001146773
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTTGGAATATGCTGTCCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MUSK (4593)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77501
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
110697429 |
- |
MUSK |
NNNTGGAATATGCTGTCCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
17891793 |
+ |
SLC5A5 |
NNNTGGAAAATGCTGTCCCT |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000005.10 |
5 |
36635710 |
- |
SLC1A3 |
NNNTGGAATTTGCTGCCCCG |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000002.12 |
2 |
222287504 |
+ |
PAX3 |
NNNTGGAAGATGCTGTCCCT |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000017.11 |
17 |
1777490 |
- |
SERPINF1 |
NNNTGGAATCTGCTGTCCCT |
NGG |
2 |
0.2333 |
Tier II |
| 6 |
NC_000010.11 |
10 |
129517799 |
- |
MGMT |
NNNGGGAATATGCTTTCCCG |
NGG |
2 |
0.0893 |
Tier II |
| 7 |
NC_000023.11 |
X |
104921053 |
- |
IL1RAPL2 |
NNNGGGAATATGGTGTCCCG |
NGG |
2 |
0.0852 |
Tier II |
| 8 |
NC_000001.11 |
1 |
226890913 |
- |
PSEN2 |
NNNTGGAATGTGCTTTCCCG |
NGG |
2 |
0.0794 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
27102856 |
+ |
Adamtsl2 |
NNNTGGAAGATGCTGTCCCC |
NGG |
2 |
0.2653 |
Tier I |
| 2 |
NC_000086.7 |
X |
9298606 |
- |
Xk |
NNNTCGAATATGCTGTCCCA |
NGG |
2 |
0.7366 |
Tier II |
| 3 |
NC_000082.6 |
16 |
94151560 |
+ |
Hlcs |
NNNTGGAGTATGCTGTCCCA |
NGG |
2 |
0.6875 |
Tier II |
| 4 |
NC_000080.6 |
14 |
32607743 |
- |
Prrxl1 |
NNNTGCAAAATGCTGTCCCG |
NGG |
2 |
0.6331 |
Tier II |
| 5 |
NC_000067.6 |
1 |
162217792 |
- |
Dnm3 |
NNNTGGAAAATCCTGTCCCG |
NGG |
2 |
0.4916 |
Tier II |
| 6 |
NC_000071.6 |
5 |
145116125 |
+ |
Arpc1b |
NNNTGGAAAATGCTGTCCCC |
NGG |
2 |
0.398 |
Tier II |
| 7 |
NC_000067.6 |
1 |
90817683 |
+ |
Col6a3 |
NNNTGGAATATGCAGTCTCG |
NGG |
2 |
0.398 |
Tier II |
| 8 |
NC_000075.6 |
9 |
103119789 |
- |
Rab6b |
NNNTGGGATGTGCTGTCCCG |
NGG |
2 |
0.3922 |
Tier II |
| 9 |
NC_000077.6 |
11 |
115818441 |
+ |
Tsen54 |
NNNTGTACTATGCTGTCCCG |
NGG |
2 |
0.2857 |
Tier II |
| 10 |
NC_000075.6 |
9 |
119003420 |
+ |
Ctdspl |
NNNTGGACTGTGCTGTCCCG |
NGG |
2 |
0.2381 |
Tier II |
| 11 |
NC_000084.6 |
18 |
7583480 |
+ |
Mpp7 |
NNNTGGAATATGCTGTGGCG |
NGG |
2 |
0.0078 |
Tier II |
| 12 |
NC_000082.6 |
16 |
94151560 |
+ |
LOC115488625 |
NNNTGGAGTATGCTGTCCCA |
NGG |
2 |
0.6875 |
Tier III |
| 13 |
NC_000067.6 |
1 |
162217792 |
- |
Dnm3os |
NNNTGGAAAATCCTGTCCCG |
NGG |
2 |
0.4916 |
Tier III |
| 14 |
NC_000075.6 |
9 |
103119789 |
- |
Gm51658 |
NNNTGGGATGTGCTGTCCCG |
NGG |
2 |
0.3922 |
Tier III |
| 15 |
NC_000085.6 |
19 |
4205490 |
+ |
LOC102631992 |
NNNGGGAATATGCTGTCACG |
NGG |
2 |
0.3365 |
Tier III |
| 16 |
NC_000079.6 |
13 |
61843491 |
+ |
Gm7240 |
NNNTGGAATATGCTGTCACC |
NGG |
2 |
0.2308 |
Tier III |
| 17 |
NC_000068.7 |
2 |
132520989 |
- |
1700026D11Rik |
NNNTGGAATATTCTTTCCCG |
NGG |
2 |
0.0549 |
Tier III |
Other clones with same target sequence:
(none)
