Construct: sgRNA BRDN0001146781
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCACTGGGGATCGTTCAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRRAP (8295)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76536
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
98948654 |
- |
TRRAP |
NNNCTGGGGATCGTTCAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
2709235 |
+ |
AMZ1 |
NNNCTGGGGATGGTTCAGTG |
NTG |
2 |
0.0173 |
Tier I |
| 3 |
NC_000023.11 |
X |
31194339 |
+ |
DMD |
NNNCTGGGGAAAGTTCAGTG |
NGG |
2 |
0.5357 |
Tier II |
| 4 |
NC_000009.12 |
9 |
122206839 |
- |
LHX6 |
NNNCTGGGGGACGTTCAGTG |
NGG |
2 |
0.4167 |
Tier II |
| 5 |
NC_000008.11 |
8 |
33426874 |
+ |
FUT10 |
NNNCTGGGGAATGTTCAGTG |
NGG |
2 |
0.4038 |
Tier II |
| 6 |
NC_000016.10 |
16 |
88761624 |
- |
PIEZO1 |
NNNCTGGGGGTCGTTCACTG |
NGG |
2 |
0.2646 |
Tier II |
| 7 |
NC_000006.12 |
6 |
165455403 |
- |
PDE10A |
NNNGTGGGGATCCTTCAGTG |
NGG |
2 |
0.2105 |
Tier II |
| 8 |
NC_000001.11 |
1 |
243478587 |
- |
SDCCAG8 |
NNNCTGAGGATCGTGCAGTG |
NGG |
2 |
0.05 |
Tier II |
| 9 |
NC_000009.12 |
9 |
98960362 |
- |
COL15A1 |
NNNCTGGGGTTCGTTCAGTG |
NTG |
2 |
0.0344 |
Tier II |
| 10 |
NC_000006.12 |
6 |
37511236 |
- |
LINC02520 |
NNNCTGGGGATCCATCAGTG |
NGG |
2 |
0.2607 |
Tier III |
| 11 |
NC_000023.11 |
X |
38918045 |
+ |
LOC124905177 |
NNNCTGGGGATTTTTCAGTG |
NGG |
2 |
0.1615 |
Tier III |
| 12 |
NC_000007.14 |
7 |
17390976 |
- |
LINC02888 |
NNNCTGGGGATCTTGCAGTG |
NGG |
2 |
0.015 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
144813522 |
- |
Trrap |
NNNCTGGGGATCATTCAGTG |
NTG |
2 |
0.036 |
Tier I |
| 2 |
NC_000069.6 |
3 |
29349412 |
+ |
Egfem1 |
NNNCTGGGGATTATTCAGTG |
NGG |
2 |
0.497 |
Tier II |
| 3 |
NC_000071.6 |
5 |
125285753 |
+ |
Scarb1 |
NNNCTGGGGATGGTTCAGTG |
NGG |
1 |
0.4444 |
Tier II |
| 4 |
NC_000080.6 |
14 |
56930858 |
+ |
Zmym2 |
NNNCTGGTGATGGTTCAGTG |
NGG |
2 |
0.2778 |
Tier II |
| 5 |
NC_000070.6 |
4 |
98713500 |
- |
Patj |
NNNCAGGGGAGCGTTCAGTG |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000075.6 |
9 |
45047383 |
+ |
Mpzl2 |
NNNCTTGGGATCTTTCAGTG |
NGG |
2 |
0.2 |
Tier II |
| 7 |
NC_000070.6 |
4 |
87060175 |
+ |
Slc24a2 |
NNNCTGGGGATGCTTCAGTG |
NGG |
2 |
0.1871 |
Tier II |
| 8 |
NC_000067.6 |
1 |
63752918 |
+ |
Fastkd2 |
NNNCTGGGGATGGTTCAGTG |
NTG |
2 |
0.0173 |
Tier II |
Other clones with same target sequence:
(none)
