Construct: sgRNA BRDN0001146782

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTACCATATATATGCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLT1 (2321)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75558

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 28431148 + FLT1 NNNACCATATATATGCACTG NGG 0 1.0 Tier I
2 NC_000016.10 16 58620158 + CNOT1 NNNACTGTATATATGCACTG NGG 2 0.6555 Tier II
3 NC_000002.12 2 173961446 + SP3 NNNACAATATATATGCAATG NGG 2 0.5 Tier II
4 NC_000015.10 15 61043608 + RORA NNNACCATAGAAATGCACTG NGG 2 0.4 Tier II
5 NC_000001.11 1 165217666 - LMX1A NNNCCCACATATATGCACTG NGG 2 0.3529 Tier II
6 NC_000008.11 8 29152969 + KIF13B NNNAACTTATATATGCACTG NGG 2 0.25 Tier II
7 NC_000016.10 16 70957805 + HYDIN NNNACCATATAAATGTACTG NGG 2 0.2462 Tier II
8 NC_000003.12 3 174266011 - NLGN1 NNNCACATATATATGCACTG NGG 2 0.2017 Tier II
9 NC_000007.14 7 126743032 + GRM8 NNNATCATATTTATGCACTG NGG 2 0.1958 Tier II
10 NC_000009.12 9 83021050 - RASEF NNNACCACATATATGCCCTG NGG 2 0.1765 Tier II
11 NC_000018.10 18 21839110 + MIB1 NNNACCATATAGATGCACTG NAG 2 0.1296 Tier II
12 NC_000002.12 2 1103158 - SNTG2 NNNACCCTATATCTGCACTG NGG 2 0.0921 Tier II
13 NC_000018.10 18 21651960 - ABHD3 NNNACCATATATATGAACTG NTG 2 0.039 Tier II
14 NC_000001.11 1 175398236 + TNR NNNAGCATATATATGCACTG NTG 2 0.0234 Tier II
15 NC_000005.10 5 172196204 - EFCAB9 NNNACCATATATATGCACAG NGA 2 0.0198 Tier II
16 NC_000002.12 2 71078849 + NAGK NNNACCATACATATGCACTG NGT 2 0.0086 Tier II
17 NC_000003.12 3 16289962 - OXNAD1 NNNACCATATACAGGCACTG NGG 2 0.0 Tier II
18 NC_000005.10 5 117452855 + LINC00992 NNNACCATATATATGCAATG NGG 1 0.5385 Tier III
19 NC_000015.10 15 61043608 + LOC107984805 NNNACCATAGAAATGCACTG NGG 2 0.4 Tier III
20 NC_000007.14 7 20052864 + LOC101927668 NNNGACATATATATGCACTG NGG 2 0.3571 Tier III
21 NC_000001.11 1 165217666 - LMX1A-AS1 NNNCCCACATATATGCACTG NGG 2 0.3529 Tier III
22 NC_000017.11 17 5907880 - LOC339166 NNNACCATATATATGAACTG NAG 2 0.2593 Tier III
23 NC_000004.12 4 14815855 + LINC00504 NNNCCCAGATATATGCACTG NGG 2 0.2588 Tier III
24 NC_000001.11 1 146759624 - HYDIN2 NNNACCATATAAATGTACTG NGG 2 0.2462 Tier III
25 NC_000016.10 16 60373130 - LOC101927605 NNNGCCATATATATGTACTG NGG 2 0.1923 Tier III
26 NC_000015.10 15 41287473 + OIP5-AS1 NNNATCATATATATGCACTG NAG 2 0.165 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 21082620 - Zfp300 NNNACCATATATATGTACTG NGT 2 0.005 Tier I
2 NC_000073.6 7 116085678 - 1110004F10Rik NNNTACATATATATGCACTG NGG 2 0.3636 Tier II
3 NC_000085.6 19 41500479 + Lcor NNNACCATATATATGTACTA NGG 2 0.2885 Tier II
4 NC_000071.6 5 107552344 + Glmn NNNACCTTATGTATGCACTG NGG 2 0.2844 Tier II
5 NC_000069.6 3 118623125 + Dpyd NNNACCATATATTTACACTG NGG 2 0.2824 Tier II
6 NC_000067.6 1 12693974 + Sulf1 NNNACCATATACCTGCACTG NGG 2 0.1994 Tier II
7 NC_000072.6 6 48481868 + Sspo NNNGCCATATATATGTACTG NGG 2 0.1923 Tier II
8 NC_000075.6 9 31338222 + Prdm10 NNNACCATATGTATGCACTG NAG 2 0.1685 Tier II
9 NC_000080.6 14 8474320 - Cfap20dc NNNACCACATATATTCACTG NGG 2 0.1429 Tier II
10 NC_000072.6 6 8315095 - Umad1 NNNACCATATATATTCACTG NGG 1 0.1429 Tier II
11 NC_000079.6 13 29473905 + Cdkal1 NNNTCCATATATCTGCACTG NGG 2 0.134 Tier II
12 NC_000077.6 11 75076826 + Smg6 NNNTCCATATATATGCAGTG NGG 2 0.0848 Tier II
13 NC_000067.6 1 187678284 - Esrrg NNNACCATATAGATTCACTG NGG 2 0.0714 Tier II
14 NC_000074.6 8 73090783 + Large1 NNNACCATTTATATGCACTG NGA 2 0.0417 Tier II
15 NC_000068.7 2 21400336 - Gpr158 NNNACCATATATATGCACTG NGC 1 0.0222 Tier II
16 NC_000074.6 8 45972708 - 1700029J07Rik NNNACCATATATATGCATTG NGC 2 0.0143 Tier II
17 NC_000068.7 2 53695954 - Gm38481 NNNCCCATATATATGCACTG NTG 2 0.0138 Tier II
18 NC_000079.6 13 8890803 + Idi1 NNNCCCATATATATGCACTG NTG 2 0.0138 Tier II
19 NC_000077.6 11 73643551 - Gm12327 NNNACCATATATATGCACTT NGG 1 0.7 Tier III
20 NC_000073.6 7 116085678 - 1700003G18Rik NNNTACATATATATGCACTG NGG 2 0.3636 Tier III
21 NC_000068.7 2 161409481 + Gm35374 NNNATCATATATATGCACTG NAG 2 0.165 Tier III
22 NC_000071.6 5 87369642 - Gm35570 NNNCCCATATATATGCACTG NTG 2 0.0138 Tier III
23 NC_000076.6 10 39911972 + Gm8911 NNNCCCATATATATGCACTG NTG 2 0.0138 Tier III
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Other clones with same target sequence:

(none)