Construct: sgRNA BRDN0001146783

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAATAACTCATCTTTAAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYK (6850)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76778

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 90877627 - SYK NNNTAACTCATCTTTAAGAG NGG 0 1.0 Tier I
2 NC_000012.12 12 117079265 - TESC NNNTAACTCATCTTTAATAG NGG 1 0.6667 Tier II
3 NC_000012.12 12 96549417 - CFAP54 NNNTAAATTATCTTTAAGAG NGG 2 0.6563 Tier II
4 NC_000001.11 1 61333658 - NFIA NNNTAACTCATCTTTAAAAA NGG 2 0.649 Tier II
5 NC_000005.10 5 31740815 - PDZD2 NNNTAACTCATTTTTAAGAA NGG 2 0.5048 Tier II
6 NC_000005.10 5 160621512 - ATP10B NNNTAATTCATTTTTAAGAG NGG 2 0.4375 Tier II
7 NC_000015.10 15 48639593 - FBN1 NNNTAACTCATCATTAAGTG NGG 2 0.3728 Tier II
8 NC_000002.12 2 124415711 - CNTNAP5 NNNTACCTCATCTTTAAGAT NGG 2 0.3182 Tier II
9 NC_000018.10 18 69608300 - DOK6 NNNCAACTCATCTTTAACAG NGG 2 0.3081 Tier II
10 NC_000023.11 X 100381287 - PCDH19 NNNTAACTCATCTTTAAGTC NGG 2 0.2308 Tier II
11 NC_000004.12 4 20707233 + PACRGL NNNTAAATCATCTTTAAGAG NAG 2 0.1944 Tier II
12 NC_000001.11 1 77342716 - AK5 NNNTAACTCATCTTTAAAAG NAG 2 0.1795 Tier II
13 NC_000008.11 8 94473460 + RAD54B NNNTTCCTCATCTTTAAGAG NGG 2 0.1653 Tier II
14 NC_000020.11 20 2520648 + ZNF343 NNNTAACTCATCTTAAAGAG NAG 2 0.1501 Tier II
15 NC_000006.12 6 154153080 - OPRM1 NNNTAATTCATCTTTATGAG NGG 2 0.1083 Tier II
16 NC_000001.11 1 94277294 - ARHGAP29 NNNTAACTCATCTTTAAGAA NGA 2 0.0651 Tier II
17 NC_000002.12 2 2211513 + MYT1L NNNTAACTCATCTTTAAGAC NGA 2 0.0298 Tier II
18 NC_000018.10 18 42013917 + PIK3C3 NNNTAACTCATCTTTAAGGG NTG 2 0.0146 Tier II
19 NC_000001.11 1 42284226 - FOXJ3 NNNTAATTCATCTTTAAGAG NGT 2 0.0131 Tier II
20 NC_000001.11 1 57971572 + DAB1 NNNTAACTCATCTGTAAGAC NGG 2 0.0 Tier II
21 NC_000009.12 9 127276925 - GARNL3 NNNTAACTCATCTGTAAGAT NGG 2 0.0 Tier II
22 NC_000006.12 6 88379417 + LOC105377885 NNNTAACTCATCCTTAAGAA NGG 2 0.7401 Tier III
23 NC_000006.12 6 88379417 + LOC124901358 NNNTAACTCATCCTTAAGAA NGG 2 0.7401 Tier III
24 NC_000014.9 14 29083011 - LOC107984685 NNNTAACTCATATTTAAGTG NGG 2 0.3846 Tier III
25 NC_000012.12 12 90085620 + LOC105369890 NNNTTACTCATCTTTAAGAT NGG 2 0.2545 Tier III
26 NC_000019.10 19 56719625 - LOC105372472 NNNTAACTCATCTTTATGTG NGG 2 0.0718 Tier III
27 NC_000001.11 1 94277294 - ARHGAP29-AS1 NNNTAACTCATCTTTAAGAA NGA 2 0.0651 Tier III
28 NC_000023.11 X 22995892 + PTCHD1-AS NNNTAACTCATCTTTAAGAG NGT 1 0.0161 Tier III
29 NC_000001.11 1 57971572 + LOC105378746 NNNTAACTCATCTGTAAGAC NGG 2 0.0 Tier III
30 NC_000003.12 3 67669247 + SUCLG2-DT NNNTATCTCATCTTTTAGAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 60772168 + Top3a NNNTAACTGATCTTTAAGAA NGG 2 0.5804 Tier II
2 NC_000086.7 X 74019762 - Irak1 NNNTGACTCATCTTTAAAAG NGG 2 0.4985 Tier II
3 NC_000073.6 7 74393565 + Slco3a1 NNNTAACTCATCATTAAGTG NGG 2 0.3728 Tier II
4 NC_000067.6 1 62780811 + Nrp2 NNNTACCTCATCTTTAAAAG NGG 2 0.3147 Tier II
5 NC_000067.6 1 178981619 + Smyd3 NNNAAACTCATCTTTAAGAC NGG 2 0.2041 Tier II
6 NC_000073.6 7 28948002 + Actn4 NNNTCACTCATCTTTGAGAG NGG 2 0.0962 Tier II
7 NC_000080.6 14 31799820 + Ankrd28 NNNTAACTCATCATTAAGAG NCG 2 0.0742 Tier II
8 NC_000074.6 8 45788052 - Sorbs2 NNNTAACTCATCTTTAAGAA NTG 2 0.0365 Tier II
9 NC_000086.7 X 140581700 - Tsc22d3 NNNTAAATCATCTTTAAGAG NTG 2 0.0292 Tier II
10 NC_000068.7 2 62159019 - Slc4a10 NNNTAACTCATATTTAAGAG NTG 2 0.0278 Tier II
11 NC_000073.6 7 28948002 + Gm51446 NNNTCACTCATCTTTGAGAG NGG 2 0.0962 Tier III
12 NC_000074.6 8 45788052 - Sorbs2os NNNTAACTCATCTTTAAGAA NTG 2 0.0365 Tier III
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Other clones with same target sequence:

(none)