Construct: sgRNA BRDN0001146785
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAATGCATGAACCTTCGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ICK (22858)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77412
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
53037950 |
- |
CILK1 |
NNNATGCATGAACCTTCGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
43523086 |
- |
ADAMTS20 |
NNNATGGATGAACCTTCAGG |
NGG |
2 |
0.3258 |
Tier II |
| 3 |
NC_000006.12 |
6 |
163571183 |
+ |
QKI |
NNNATGCATGAACCTTCGGT |
NAG |
2 |
0.1815 |
Tier II |
| 4 |
NC_000003.12 |
3 |
21859876 |
- |
ZNF385D |
NNNATGCATGAATCTTCGGG |
NAG |
2 |
0.1815 |
Tier II |
| 5 |
NC_000009.12 |
9 |
72108083 |
+ |
BTF3P4 |
NNNATGCCTGAACCTTAGGG |
NGG |
2 |
0.2 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
78135640 |
+ |
Cilk1 |
NNNATGCATGAACCTTCGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000085.6 |
19 |
31550078 |
+ |
Prkg1 |
NNNATGCATGAGCCTACGGG |
NGG |
2 |
0.6566 |
Tier II |
| 3 |
NC_000083.6 |
17 |
68600817 |
- |
L3mbtl4 |
NNNATGCTTGAACCTTCTGG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000083.6 |
17 |
10209155 |
- |
Qk |
NNNATGCATGAACCTTCGGT |
NAG |
2 |
0.1815 |
Tier II |
| 5 |
NC_000082.6 |
16 |
7108187 |
- |
Rbfox1 |
NNNATGCATGAACCTTCAGG |
NAG |
2 |
0.1795 |
Tier II |
| 6 |
NC_000067.6 |
1 |
158879008 |
- |
Pappa2 |
NNNATGCATGAACCTACGGG |
NGC |
2 |
0.0202 |
Tier II |
| 7 |
NC_000085.6 |
19 |
16443888 |
+ |
Gna14 |
NNNATGCATGACCCTTGGGG |
NGG |
2 |
0.0155 |
Tier II |
| 8 |
NC_000077.6 |
11 |
34304802 |
- |
Dock2 |
NNNATGCATGAACCTTGGGG |
NGT |
2 |
0.0009 |
Tier II |
| 9 |
NC_000068.7 |
2 |
152142028 |
+ |
Gm31535 |
NNNATGAATGAACCTTCTGG |
NGG |
2 |
0.5 |
Tier III |
| 10 |
NC_000076.6 |
10 |
42987631 |
- |
Gm5079 |
NNNATGCATGGACCTTCGGG |
NGT |
2 |
0.0105 |
Tier III |
| 11 |
NC_000077.6 |
11 |
34304802 |
- |
4930403D09Rik |
NNNATGCATGAACCTTGGGG |
NGT |
2 |
0.0009 |
Tier III |
Other clones with same target sequence:
(none)
