Construct: sgRNA BRDN0001146786

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CACCAAACTCCTGAGCATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD4 (23476)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77346

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 15263520 + BRD4 NNNCAAACTCCTGAGCATCA NGG 0 1.0 Tier I
2 NC_000006.12 6 13365567 - GFOD1 NNNCAAGCTCATGAGCATCA NGG 2 0.5294 Tier I
3 NC_000006.12 6 32977471 - BRD2 NNNCAAACTCCTGTGCATCC NGG 2 0.1212 Tier I
4 NC_000001.11 1 14598999 + KAZN NNNCAAAATCCTGAGCATGA NGG 2 0.0813 Tier I
5 NC_000005.10 5 73870109 - ARHGEF28 NNNCAAACTCCTGGGCATCA NTG 2 0.0182 Tier I
6 NC_000012.12 12 2674936 - CACNA1C NNNCAAACCCCTAAGCATCA NGG 2 0.8521 Tier II
7 NC_000002.12 2 162797701 + KCNH7 NNNCAAACACCAGAGCATCA NGG 2 0.7429 Tier II
8 NC_000004.12 4 139828557 + MAML3 NNNCAAAGCCCTGAGCATCA NGG 2 0.5934 Tier II
9 NC_000006.12 6 112205914 + LAMA4 NNNAAAACTCCTGAGCAACA NGG 2 0.5614 Tier II
10 NC_000013.11 13 43554781 - ENOX1 NNNCAGACTCCTGAGCAACA NGG 2 0.4762 Tier II
11 NC_000002.12 2 77242350 + LRRTM4 NNNAAAACTCCTGTGCATCA NGG 2 0.4491 Tier II
12 NC_000016.10 16 28041236 - GSG1L NNNCAAACTCCTGAGCACCA NGG 1 0.4286 Tier II
13 NC_000002.12 2 87864428 - RGPD2 NNNCAAAGGCCTGAGCATCA NGG 2 0.398 Tier II
14 NC_000013.11 13 30733966 - ALOX5AP NNNCAAAGGCCTGAGCATCA NGG 2 0.398 Tier II
15 NC_000005.10 5 11626246 - CTNND2 NNNAAAACTCCTGAGCATTA NGG 2 0.3887 Tier II
16 NC_000001.11 1 20038667 - PLA2G5 NNNCAGTCTCCTGAGCATCA NGG 2 0.3125 Tier II
17 NC_000005.10 5 170599592 - KCNIP1 NNNCACAGTCCTGAGCATCA NGG 2 0.2922 Tier II
18 NC_000005.10 5 156359600 - SGCD NNNCAGACTGCTGAGCATCA NGG 2 0.2778 Tier II
19 NC_000009.12 9 91869188 + ROR2 NNNCAAACTCCTGAGCACCT NGG 2 0.2571 Tier II
20 NC_000002.12 2 101254786 - CNOT11 NNNCAAACTCCTGAGCAGCT NGG 2 0.2 Tier II
21 NC_000009.12 9 6431492 - UHRF2 NNNCAAACTCCTGAGCAGCT NGG 2 0.2 Tier II
22 NC_000010.11 10 1009780 - GTPBP4 NNNCAAACTCCTGGGCATAA NGG 2 0.2 Tier II
23 NC_000023.11 X 56066471 - KLF8 NNNCACCCTCCTGAGCATCA NGG 2 0.1989 Tier II
24 NC_000003.12 3 189206734 + TPRG1 NNNCAAACTCCTGAGCACAA NGG 2 0.1837 Tier II
25 NC_000012.12 12 22675454 + ETNK1 NNNCAAACTCCTGAGCACAA NGG 2 0.1837 Tier II
26 NC_000018.10 18 79699073 - CTDP1 NNNCAAACTCCTGTGCAGCA NGG 2 0.1778 Tier II
27 NC_000001.11 1 243615973 + AKT3 NNNCAAACTCCTGAGGATCA NGG 1 0.1538 Tier II
28 NC_000002.12 2 229172862 + PID1 NNNCAACCTCCTGAGCAGCA NGG 2 0.1458 Tier II
29 NC_000019.10 19 3026054 - TLE2 NNNCAAACTTCTGAGCTTCA NGG 2 0.1255 Tier II
30 NC_000019.10 19 45389250 + PPP1R13L NNNCAAAATCCTGAGCCTCA NGG 2 0.1147 Tier II
31 NC_000018.10 18 45664820 - SLC14A2 NNNCAAACTCCTGAGCTACA NGG 2 0.0889 Tier II
32 NC_000015.10 15 90949489 + UNC45A NNNCCAACTCCTGAGCCTCA NGG 2 0.0882 Tier II
33 NC_000011.10 11 85712951 + SYTL2 NNNCAAACTCCTGGGCCTCA NGG 2 0.0824 Tier II
34 NC_000016.10 16 46925145 + GPT2 NNNCAAACTCCTGGGCCTCA NGG 2 0.0824 Tier II
35 NC_000019.10 19 31223031 - TSHZ3 NNNCAACCTCCTGAGCCTCA NGG 2 0.0772 Tier II
36 NC_000005.10 5 37168570 + CPLANE1 NNNCAAACTCCTGGGGATCA NGG 2 0.0718 Tier II
37 NC_000019.10 19 49417837 - KASH5 NNNCCAACTCCTGAGCATGA NGG 2 0.0625 Tier II
38 NC_000008.11 8 124581239 - MTSS1 NNNCAAACTCCTGGGCTTCA NGG 2 0.0622 Tier II
39 NC_000011.10 11 9522324 + ZNF143 NNNCAAACTCCTGGGCTTCA NGG 2 0.0622 Tier II
40 NC_000014.9 14 45059276 + TOGARAM1 NNNCAAACTCCTGGGCTTCA NGG 2 0.0622 Tier II
41 NC_000016.10 16 69124734 + CHTF8 NNNCAAACTCCTGAGCTCCA NGG 2 0.0571 Tier II
42 NC_000016.10 16 69124734 + DERPC NNNCAAACTCCTGAGCTCCA NGG 2 0.0571 Tier II
43 NC_000004.12 4 84865855 - WDFY3 NNNCAAACTCCTGAGCTTAA NGG 2 0.0571 Tier II
44 NC_000005.10 5 138373125 - KDM3B NNNCAAACTCCTGAGCTTAA NGG 2 0.0571 Tier II
45 NC_000010.11 10 96430661 - TLL2 NNNCAAACTCCTGAGCTTAA NGG 2 0.0571 Tier II
46 NC_000023.11 X 107829112 + MID2 NNNCAAACTCCTGAGCTTAA NGG 2 0.0571 Tier II
47 NC_000004.12 4 102297839 - SLC39A8 NNNCAAACTCCTTAGCCTCA NGG 2 0.0529 Tier II
48 NC_000009.12 9 132015374 + MED27 NNNCAAACTGCTGAGCTTCA NGG 2 0.0519 Tier II
49 NC_000001.11 1 179975313 - CEP350 NNNCAAACTCCTTAGCATGA NGG 2 0.0375 Tier II
50 NC_000017.11 17 37154227 - ACACA NNNCAAACTCCTGAGCTTCA NAG 2 0.0346 Tier II
51 NC_000017.11 17 35830235 - TAF15 NNNCGAACTCCTGAGCATCA NTG 2 0.0281 Tier II
52 NC_000017.11 17 39379701 - FBXL20 NNNCAAACTCCTGAGGCTCA NGG 2 0.0271 Tier II
53 NC_000003.12 3 125133806 + SLC12A8 NNNCAAACTCCTGAGCTTGA NGG 2 0.0167 Tier II
54 NC_000005.10 5 38493022 + LIFR NNNCAAGCTCCTGAGCATCA NGC 2 0.0157 Tier II
55 NC_000005.10 5 37409738 + WDR70 NNNCAAACTCCTGAACATCA NGT 2 0.0152 Tier II
56 NC_000022.11 22 48686585 - TAFA5 NNNCAAACACCTGAGCATCA NGT 2 0.015 Tier II
57 NC_000020.11 20 46009572 - MMP9 NNNCAAACTCCTGAGCTTCA NCG 2 0.0143 Tier II
58 NC_000005.10 5 153637531 - GRIA1 NNNCAAACTCCTTAGCATCA NTG 2 0.0117 Tier II
59 NC_000017.11 17 29539043 - TAOK1 NNNCAAACTCCTGAGCACCA NGT 2 0.0069 Tier II
60 NC_000002.12 2 61947469 - COMMD1 NNNCAAACTCCTGACCATCA NGC 2 0.0061 Tier II
61 NC_000001.11 1 100028561 + SLC35A3 NNNCAAACTCCTGAGCTTCA NTG 2 0.0052 Tier II
62 NC_000015.10 15 37030873 + MEIS2 NNNCAAACTCCTGAGCTTCA NGC 2 0.003 Tier II
63 NC_000006.12 6 15583078 + DTNBP1 NNNCAAACTCCTGAGCCTCA NGT 2 0.0028 Tier II
64 NC_000003.12 3 50332573 - RASSF1 NNNCAAACTCCTGAGGATCA NGT 2 0.0025 Tier II
65 NC_000002.12 2 162797701 + KCNH7-AS1 NNNCAAACACCAGAGCATCA NGG 2 0.7429 Tier III
66 NC_000010.11 10 70944438 - LINC02622 NNNCAAAATCCTGAGCATCA NGG 1 0.65 Tier III
67 NC_000018.10 18 9408019 - LOC105371980 NNNCAAACTCCTGAGAATAA NGG 2 0.4286 Tier III
68 NC_000013.11 13 30733966 - LOC124903146 NNNCAAAGGCCTGAGCATCA NGG 2 0.398 Tier III
69 NC_000005.10 5 156359600 - LOC124901120 NNNCAGACTGCTGAGCATCA NGG 2 0.2778 Tier III
70 NC_000023.11 X 56066471 - LOC124900486 NNNCACCCTCCTGAGCATCA NGG 2 0.1989 Tier III
71 NC_000016.10 16 48689022 - LOC105371240 NNNCAGACTCCTGAGCGTCA NGG 2 0.1261 Tier III
72 NC_000010.11 10 91597840 + HECTD2-AS1 NNNCAAACTCCTGAGCATGA NGG 1 0.125 Tier III
73 NC_000018.10 18 45664820 - LOC105372093 NNNCAAACTCCTGAGCTACA NGG 2 0.0889 Tier III
74 NC_000004.12 4 10367424 - LOC124900667 NNNCAAACTCCTGACCTTCA NGG 2 0.0364 Tier III
75 NC_000012.12 12 11436907 - LOC440084 NNNCAAACTCCTGAGCTTGA NGG 2 0.0167 Tier III
76 NC_000017.11 17 50287807 - TMEM92-AS1 NNNCAAACTCCTGAGCTTCA NCG 2 0.0143 Tier III
77 NC_000002.12 2 17870835 - LOC124907737 NNNCAAACTCCTGACCATCA NTG 2 0.0106 Tier III
78 NC_000002.12 2 61947469 - LOC124906012 NNNCAAACTCCTGACCATCA NGC 2 0.0061 Tier III
79 NC_000001.11 1 100028561 + LOC124904230 NNNCAAACTCCTGAGCTTCA NTG 2 0.0052 Tier III
80 NC_000009.12 9 38426556 + LOC105376041 NNNCAAACTCCTGAGCCTCA NGC 2 0.0039 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 76721854 - Col6a1 NNNCAAACACCTGGGCATCA NGG 2 0.4333 Tier I
2 NC_000067.6 1 88228260 + Mroh2a NNNCAACCTCCTGAACATCA NGG 2 0.4118 Tier I
3 NC_000075.6 9 79898215 - Filip1 NNNCCAGCTCCTGAGCATCA NGG 2 0.3529 Tier I
4 NC_000081.6 15 8247123 - Cplane1 NNNCAAACTTCTGAGCAGCA NGG 2 0.3137 Tier I
5 NC_000083.6 17 34114450 + Brd2 NNNCAAACTCCTGTGCATCC NGG 2 0.1212 Tier I
6 NC_000071.6 5 113957425 - Ssh1 NNNCAAACTCCGGAGCATCA NGA 2 0.0347 Tier I
7 NC_000079.6 13 97965509 + Arhgef28 NNNCAAACTCCTGAGCATCA NTA 2 0.0 Tier I
8 NC_000083.6 17 78725699 + Strn NNNCAAACTCCAGAACATCA NGG 2 0.7529 Tier II
9 NC_000080.6 14 55520420 - Nrl NNNCAAACTTCTGAGCATCG NGG 2 0.7197 Tier II
10 NC_000081.6 15 10309411 + Prlr NNNCAGACTCCTGAGAATCA NGG 2 0.7143 Tier II
11 NC_000072.6 6 45102290 - Cntnap2 NNNCATACTCCTGAGCATCA NGG 1 0.7143 Tier II
12 NC_000068.7 2 72030089 + Rapgef4 NNNCAAAGTCCTGAGCATCA NGG 1 0.6429 Tier II
13 NC_000073.6 7 92292217 + Dlg2 NNNCAAACTCCTGGACATCA NGG 2 0.4392 Tier II
14 NC_000086.7 X 73677963 + Slc6a8 NNNCAAACTCCAGTGCATCA NGG 2 0.4267 Tier II
15 NC_000080.6 14 65972961 - Clu NNNCAACCTCCTAAGCATCA NGG 2 0.4038 Tier II
16 NC_000081.6 15 37370569 + Ncald NNNCAAACTGCTGAGAATCA NGG 2 0.3889 Tier II
17 NC_000074.6 8 47550971 + Rwdd4a NNNGAAGCTCCTGAGCATCA NGG 2 0.3529 Tier II
18 NC_000082.6 16 21535975 - Vps8 NNNCAAACTCATGAGCACCA NGG 2 0.3214 Tier II
19 NC_000070.6 4 74028802 - Frmd3 NNNCATACTCCTGAGCATAA NGG 2 0.3061 Tier II
20 NC_000076.6 10 82639134 + Tdg NNNCAAACCCCTGAGTATCA NGG 2 0.284 Tier II
21 NC_000082.6 16 33202463 - Osbpl11 NNNCAAACTCCTGGGCATCT NGG 2 0.28 Tier II
22 NC_000070.6 4 111215420 + Agbl4 NNNCAAACTCCTGAGCACCT NGG 2 0.2571 Tier II
23 NC_000077.6 11 83284455 + Slfn14 NNNCAGACTCCTGAGCAGCA NGG 2 0.2381 Tier II
24 NC_000070.6 4 43331874 + Atp8b5 NNNGAAACTCCTCAGCATCA NGG 2 0.2105 Tier II
25 NC_000067.6 1 54861195 - Ankrd44 NNNCAAACTCCTGGGCATAA NGG 2 0.2 Tier II
26 NC_000081.6 15 92458797 + Pdzrn4 NNNCAAACTCCTTAGCAACA NGG 2 0.2 Tier II
27 NC_000069.6 3 146739778 + Prkacb NNNCAATCTCCTGAGCAGCA NGG 2 0.1458 Tier II
28 NC_000077.6 11 119352906 - Sgsh NNNCCAACTCCTGAGCATCA NAG 2 0.1296 Tier II
29 NC_000077.6 11 3407288 + Limk2 NNNCAAATTCCTGATCATCA NGG 2 0.125 Tier II
30 NC_000085.6 19 39506104 + Cyp2c39 NNNCAAACTCCTGGGCATCC NGG 2 0.1061 Tier II
31 NC_000081.6 15 32425308 - Sema5a NNNCAAACTCCTGAGCTTCG NGG 2 0.102 Tier II
32 NC_000068.7 2 72139663 + Rapgef4 NNNCAAACTCCTGACCATCC NGG 2 0.062 Tier II
33 NC_000084.6 18 12296410 - Ankrd29 NNNCAAACTCCTGAGCATCG NGA 2 0.0531 Tier II
34 NC_000083.6 17 24735207 + Rpl3l NNNCAAACTTCTGAGCATCA NTG 2 0.0367 Tier II
35 NC_000071.6 5 72851829 + Tec NNNCAAACTCCTGAGCAACA NTG 2 0.026 Tier II
36 NC_000067.6 1 105676515 - Relch NNNCAAACTCTTGAGCATCA NGA 2 0.0214 Tier II
37 NC_000068.7 2 105671379 + Pax6 NNNCAAAATCCTGAGCATCA NGC 2 0.0144 Tier II
38 NC_000075.6 9 35017345 + Kirrel3 NNNCAAACTCCTGAGCTTCA NGT 2 0.0022 Tier II
39 NC_000083.6 17 87097329 + Gm30510 NNNCAAACTCCTGAACATCT NGG 2 0.5647 Tier III
40 NC_000072.6 6 95871540 + Gm32009 NNNCAAATTCTTGAGCATCA NGG 2 0.2692 Tier III
41 NC_000069.6 3 17033271 + Gm42185 NNNCAAACTCCTCAGCATCT NGG 2 0.2526 Tier III
42 NC_000070.6 4 89372657 + Gm12610 NNNCGAACTCCTGAGCATCC NGG 2 0.1636 Tier III
43 NC_000077.6 11 3407288 + Gm51879 NNNCAAATTCCTGATCATCA NGG 2 0.125 Tier III
44 NC_000069.6 3 149245907 - Gm30382 NNNCAAACTCCTGAGGACCA NGG 2 0.0659 Tier III
Download CSV

Other clones with same target sequence:

(none)