Construct: sgRNA BRDN0001146791
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATATTTAGGACCTATCCAGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GTF2H1 (2965)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76598
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
18339653 |
+ |
GTF2H1 |
NNNTTTAGGACCTATCCAGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
57510925 |
- |
DNAH12 |
NNNTTTAGTACCTATTCAGC |
NGG |
2 |
0.1641 |
Tier I |
| 3 |
NC_000014.9 |
14 |
75634993 |
- |
FLVCR2 |
NNNTTTTGGACCTATCCAGC |
NAG |
2 |
0.1134 |
Tier I |
| 4 |
NC_000009.12 |
9 |
70704126 |
+ |
TRPM3 |
NNNTTTAGAACCTATCCATC |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000009.12 |
9 |
70722119 |
+ |
TRPM3 |
NNNTTTAGAACCTATCCATC |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000009.12 |
9 |
127773366 |
+ |
SH2D3C |
NNNTTTAGGACCCATCCAGC |
NGC |
2 |
0.0175 |
Tier II |
| 7 |
NC_000007.14 |
7 |
40988545 |
- |
SUGCT |
NNNTTTAGGACCTATACAGC |
NGT |
2 |
0.0161 |
Tier II |
| 8 |
NC_000003.12 |
3 |
181634592 |
+ |
SOX2-OT |
NNNTTTAGTTCCTATCCAGC |
NGG |
2 |
0.4706 |
Tier III |
| 9 |
NC_000009.12 |
9 |
70704126 |
+ |
LOC105376078 |
NNNTTTAGAACCTATCCATC |
NGG |
2 |
0.4286 |
Tier III |
| 10 |
NC_000018.10 |
18 |
60197153 |
+ |
LOC342784 |
NNNTTTAGGACCTATCCAGC |
NGT |
1 |
0.0161 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
46806825 |
+ |
Gtf2h1 |
NNNTTTAGGACCTATCCAGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000070.6 |
4 |
47473844 |
- |
Alg2 |
NNNTTTAGGAACTGTCCAGC |
NGG |
2 |
0.35 |
Tier II |
| 3 |
NC_000067.6 |
1 |
125696269 |
- |
Gpr39 |
NNNTTTAGAACCTGTCCAGC |
NGG |
2 |
0.3 |
Tier II |
| 4 |
NC_000073.6 |
7 |
102280050 |
+ |
Stim1 |
NNNCTTAGGACCTACCCAGC |
NGG |
2 |
0.1765 |
Tier II |
| 5 |
NC_000077.6 |
11 |
46185432 |
+ |
Gm35624 |
NNNTTTAAGACCTATACAGC |
NGG |
2 |
1.0 |
Tier III |
| 6 |
NC_000085.6 |
19 |
28692826 |
- |
D930032P07Rik |
NNNTTTAGCACCTATCCAGT |
NGG |
2 |
0.1615 |
Tier III |
| 7 |
NC_000069.6 |
3 |
8215903 |
- |
Gm7103 |
NNNTTTAGGACGTATCCAGT |
NGG |
2 |
0.1333 |
Tier III |
| 8 |
NC_000079.6 |
13 |
38869533 |
+ |
Gm46392 |
NNNTTTTGGAGCTATCCAGC |
NGG |
2 |
0.1094 |
Tier III |
| 9 |
NC_000073.6 |
7 |
120588221 |
+ |
Gm4105 |
NNNTTTAGGACCTATCCAGC |
NGT |
1 |
0.0161 |
Tier III |
| 10 |
NC_000073.6 |
7 |
120588221 |
+ |
Gm46020 |
NNNTTTAGGACCTATCCAGC |
NGT |
1 |
0.0161 |
Tier III |
Other clones with same target sequence:
(none)
