Construct: sgRNA BRDN0001146797
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGAAGCCCATAACGCCAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AXL (558)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76700
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
41231012 |
+ |
AXL |
NNNAGCCCATAACGCCAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
65084799 |
+ |
ZFPL1 |
NNNAGCCAATCACGCCAAGG |
NGG |
2 |
0.26 |
Tier I |
| 3 |
NC_000011.10 |
11 |
132261892 |
+ |
NTM |
NNNAACCCATAAAGCCAAGG |
NGG |
2 |
0.3333 |
Tier II |
| 4 |
NC_000010.11 |
10 |
121744983 |
- |
ATE1 |
NNNAGCCCAAAACTCCAAGG |
NGG |
2 |
0.2286 |
Tier II |
| 5 |
NC_000020.11 |
20 |
43054488 |
+ |
PTPRT |
NNNAGCCCAGAACGTCAAGG |
NGG |
2 |
0.0333 |
Tier II |
| 6 |
NC_000011.10 |
11 |
90379460 |
+ |
DISC1FP1 |
NNNAGCCCAAAAGGCCAAGG |
NGG |
2 |
0.1169 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
6084354 |
- |
Zfpl1 |
NNNAGCCAATCACGCCAAGG |
NGG |
2 |
0.26 |
Tier I |
| 2 |
NC_000070.6 |
4 |
41632355 |
- |
Dnaic1 |
NNNAGCCCATAACACAAAGG |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000068.7 |
2 |
179566552 |
+ |
Cdh4 |
NNNAGTCCATAATGCCAAGG |
NGG |
2 |
0.65 |
Tier II |
| 4 |
NC_000068.7 |
2 |
105348797 |
- |
Them7 |
NNNAGACCCTAACGCCAAGG |
NGG |
2 |
0.5306 |
Tier II |
| 5 |
NC_000080.6 |
14 |
65611040 |
- |
Nuggc |
NNNAGCACAGAACGCCAAGG |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000076.6 |
10 |
40648684 |
+ |
Ddo |
NNNAGCCCATAGCGCCAGGG |
NGG |
2 |
0.2889 |
Tier II |
| 7 |
NC_000075.6 |
9 |
116632067 |
- |
Rbms3 |
NNNAGCCCATAATGCCAAGG |
NAG |
2 |
0.1815 |
Tier II |
| 8 |
NC_000071.6 |
5 |
102570978 |
+ |
Arhgap24 |
NNNAGCCCATAAAGCCAAGG |
NAG |
2 |
0.0997 |
Tier II |
| 9 |
NC_000071.6 |
5 |
76190711 |
- |
Tmem165 |
NNNAGGCCATAAGGCCAAGG |
NGG |
2 |
0.0682 |
Tier II |
Other clones with same target sequence:
(none)
