Construct: sgRNA BRDN0001146798
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTACATATTATAGCAATCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROCK1 (6093)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77595
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
21028781 |
- |
ROCK1 |
NNNCATATTATAGCAATCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
79560881 |
- |
ROBO1 |
NNNCATATCATAGCAATTGT |
NGG |
2 |
0.5934 |
Tier II |
| 3 |
NC_000002.12 |
2 |
39235796 |
- |
CDKL4 |
NNNAATATTATAGCAATTGT |
NGG |
2 |
0.5414 |
Tier II |
| 4 |
NC_000012.12 |
12 |
75156774 |
- |
KCNC2 |
NNNCATATTATAGAAATGGT |
NGG |
2 |
0.0467 |
Tier II |
| 5 |
NC_000008.11 |
8 |
90064068 |
- |
CALB1 |
NNNCATATTATAGCAATAGT |
NGC |
2 |
0.012 |
Tier II |
| 6 |
NC_000008.11 |
8 |
90064068 |
- |
LOC124901976 |
NNNCATATTATAGCAATAGT |
NGC |
2 |
0.012 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
10122614 |
- |
Rock1 |
NNNCATATTATAGCAATCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
50492602 |
+ |
Nell1 |
NNNCATATTATAGCAAACAT |
NGG |
2 |
0.381 |
Tier II |
| 3 |
NC_000080.6 |
14 |
47294562 |
- |
Socs4 |
NNNCATATTTTAGCAATCGT |
NGC |
2 |
0.0196 |
Tier II |
| 4 |
NC_000067.6 |
1 |
185451895 |
- |
Gm2061 |
NNNCATATGATAGCAATAGT |
NGG |
2 |
0.3333 |
Tier III |
| 5 |
NC_000086.7 |
X |
36194521 |
+ |
Gm42406 |
NNNCATATTATACCAATCAT |
NGG |
2 |
0.3008 |
Tier III |
| 6 |
NC_000079.6 |
13 |
13275735 |
+ |
Gm5374 |
NNNCATATTATAGCAATCGT |
NGA |
1 |
0.0694 |
Tier III |
Other clones with same target sequence:
(none)
