Construct: sgRNA BRDN0001146799
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGAGGTTACAACCAACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA4 (2043)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76667
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
221456618 |
- |
EPHA4 |
NNNGAGGTTACAACCAACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
61727628 |
+ |
PTPRG |
NNNTAGGTTACAGCCAACAC |
NGG |
2 |
0.2372 |
Tier II |
| 3 |
NC_000002.12 |
2 |
99599589 |
+ |
AFF3 |
NNNGAGGTTCCAGCCAACAC |
NGG |
2 |
0.2174 |
Tier II |
| 4 |
NC_000019.10 |
19 |
1254746 |
- |
MIDN |
NNNGAGGTCACAACCAACCC |
NGG |
2 |
0.191 |
Tier II |
| 5 |
NC_000009.12 |
9 |
116451124 |
- |
ASTN2 |
NNNGAGCTTAGAACCAACAC |
NGG |
2 |
0.1719 |
Tier II |
| 6 |
NC_000001.11 |
1 |
212413868 |
+ |
PACC1 |
NNNGAGGTTACAGCAAACAC |
NGG |
2 |
0.1449 |
Tier II |
| 7 |
NC_000020.11 |
20 |
36240984 |
- |
AAR2 |
NNNGAGGCTACAACGAACAC |
NGG |
2 |
0.05 |
Tier II |
| 8 |
NC_000006.12 |
6 |
135038635 |
+ |
HBS1L |
NNNGAGGTTACAACAAACAC |
NGA |
2 |
0.0154 |
Tier II |
| 9 |
NC_000001.11 |
1 |
233633171 |
+ |
KCNK1 |
NNNGAGGTTACAAGCAATAC |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000006.12 |
6 |
79405343 |
- |
LOC100506851 |
NNNTAGGTTATAACCAACAC |
NGG |
2 |
0.1119 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
89603604 |
- |
Agap1 |
NNNGAGGCTGCAACCAACAC |
NGG |
2 |
0.5556 |
Tier II |
| 2 |
NC_000069.6 |
3 |
106529243 |
+ |
Cept1 |
NNNGAAGTTACATCCAACAC |
NGG |
2 |
0.3 |
Tier II |
| 3 |
NC_000076.6 |
10 |
32097832 |
- |
Nkain2 |
NNNGAGGTTACAACCACCCC |
NGG |
2 |
0.0365 |
Tier II |
| 4 |
NC_000076.6 |
10 |
89591873 |
+ |
Slc17a8 |
NNNGAGGTTAGAACCCACAC |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000074.6 |
8 |
126903304 |
+ |
Gm31718 |
NNNAAGGTTACAACCAACAG |
NGG |
2 |
0.0529 |
Tier III |
| 6 |
NC_000080.6 |
14 |
8403561 |
- |
Gm41095 |
NNNGAGGTTAAAACTAACAC |
NGG |
2 |
0.05 |
Tier III |
Other clones with same target sequence:
(none)
