Construct: sgRNA BRDN0001146802
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAATGATCAACCTTTATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMP2K (55589)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75929
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
78847233 |
+ |
BMP2K |
NNNATGATCAACCTTTATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
50764926 |
- |
ANKRD40CL |
NNNATAATCAACCTTCATGG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000008.11 |
8 |
3905339 |
- |
CSMD1 |
NNNATGATCAACTTTTATGT |
NGG |
2 |
0.49 |
Tier II |
| 4 |
NC_000018.10 |
18 |
53724814 |
- |
LOC124904304 |
NNNATGATCAACCTTTAAGC |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000002.12 |
2 |
45007765 |
+ |
SIX2 |
NNNATGATCAACCTTTAGGC |
NGG |
2 |
0.1429 |
Tier II |
| 6 |
NC_000003.12 |
3 |
132688035 |
- |
NPHP3 |
NNNATGTTCAACCTTTATGG |
NGC |
2 |
0.0097 |
Tier II |
| 7 |
NC_000002.12 |
2 |
28381037 |
+ |
LOC105374383 |
NNNAGGATCAACCTTTATGG |
NGT |
2 |
0.0103 |
Tier III |
| 8 |
NC_000003.12 |
3 |
132688035 |
- |
NPHP3-ACAD11 |
NNNATGTTCAACCTTTATGG |
NGC |
2 |
0.0097 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
97040718 |
+ |
Bmp2k |
NNNATGATCAACCTTTATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
64877465 |
- |
Fam189a1 |
NNNTTGATCAACCTTTATGA |
NGG |
2 |
0.5966 |
Tier II |
| 3 |
NC_000073.6 |
7 |
56213197 |
+ |
Herc2 |
NNNATGATCAGCCTTCATGG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000070.6 |
4 |
46710065 |
+ |
Gabbr2 |
NNNCTGATCAAGCTTTATGG |
NGG |
2 |
0.1569 |
Tier II |
| 5 |
NC_000074.6 |
8 |
110629105 |
+ |
Vac14 |
NNNCTGATCAAGCTTTATGG |
NGG |
2 |
0.1569 |
Tier II |
| 6 |
NC_000069.6 |
3 |
83999647 |
- |
Tmem131l |
NNNATGATCAGCCTTTCTGG |
NGG |
2 |
0.1147 |
Tier II |
| 7 |
NC_000074.6 |
8 |
114561146 |
- |
Wwox |
NNNATGATCACCCTTTATGG |
NGC |
2 |
0.0089 |
Tier II |
| 8 |
NC_000069.6 |
3 |
87291711 |
- |
Gm31879 |
NNNATGAACAACATTTATGG |
NGG |
2 |
0.3077 |
Tier III |
| 9 |
NC_000069.6 |
3 |
143355851 |
- |
Gm40159 |
NNNATGATGAACCTTTATGC |
NGG |
2 |
0.2653 |
Tier III |
| 10 |
NC_000072.6 |
6 |
114050412 |
+ |
Gm15083 |
NNNATGATCAACCCTCATGG |
NGG |
2 |
0.1905 |
Tier III |
| 11 |
NC_000076.6 |
10 |
12086963 |
- |
Gm40601 |
NNNATGATCAACCTTTATTG |
NGC |
2 |
0.0148 |
Tier III |
Other clones with same target sequence:
(none)
