Construct: sgRNA BRDN0001146808
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCATCGCAGATGGTGACAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MKNK1 (8569)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75512
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
46583289 |
- |
MKNK1 |
NNNTCGCAGATGGTGACAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
21868787 |
- |
MTAP |
NNNTCACAGATGGTAACAGG |
NGG |
2 |
0.9412 |
Tier II |
| 3 |
NC_000020.11 |
20 |
38229416 |
+ |
KIAA1755 |
NNNTGGCAGATAGTGACAGG |
NGG |
2 |
0.56 |
Tier II |
| 4 |
NC_000002.12 |
2 |
168188327 |
- |
STK39 |
NNNTCTCAGATGGTGACAGT |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000006.12 |
6 |
101402095 |
- |
GRIK2 |
NNNTCGCAGGAGGTGACAGG |
NGG |
2 |
0.4167 |
Tier II |
| 6 |
NC_000018.10 |
18 |
23466469 |
+ |
RIOK3 |
NNNTAGCAGATGGAGACAGG |
NGG |
2 |
0.3537 |
Tier II |
| 7 |
NC_000009.12 |
9 |
129109181 |
- |
CRAT |
NNNTCGCAGATTCTGACAGG |
NGG |
2 |
0.1619 |
Tier II |
| 8 |
NC_000002.12 |
2 |
88125504 |
- |
FABP1 |
NNNTGGCAGATGGTGGCAGG |
NGG |
2 |
0.1154 |
Tier II |
| 9 |
NC_000002.12 |
2 |
29563673 |
- |
ALK |
NNNTCGCAGATGGTGAGAGT |
NGG |
2 |
0.0412 |
Tier II |
| 10 |
NC_000003.12 |
3 |
121612230 |
- |
FBXO40 |
NNNTGGCAGATGGTGACAGG |
NGC |
2 |
0.0133 |
Tier II |
| 11 |
NC_000004.12 |
4 |
184283529 |
+ |
LOC105377582 |
NNNCTGCAGATGGTGACAGG |
NGG |
2 |
0.4118 |
Tier III |
| 12 |
NC_000003.12 |
3 |
18077434 |
+ |
BALR6 |
NNNTCGGAGATGGAGACAGG |
NGG |
2 |
0.2913 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
11855570 |
+ |
Olfr1418 |
NNNTCGCAGAGGGTGACAGA |
NGG |
2 |
0.375 |
Tier I |
| 2 |
NC_000071.6 |
5 |
35729785 |
- |
Sh3tc1 |
NNNTCACAGATGGAGACAGG |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000075.6 |
9 |
79792928 |
- |
Tmem30a |
NNNTCCCAGAAGGTGACAGG |
NGG |
2 |
0.5114 |
Tier II |
| 4 |
NC_000078.6 |
12 |
108266027 |
+ |
Ccdc85c |
NNNCCGCACATGGTGACAGG |
NGG |
2 |
0.3484 |
Tier II |
| 5 |
NC_000069.6 |
3 |
79818498 |
- |
Tmem144 |
NNNTCGCAGATGGTGATAAG |
NGG |
2 |
0.3333 |
Tier II |
| 6 |
NC_000084.6 |
18 |
15030430 |
- |
Kctd1 |
NNNTCGGAGATGGAGACAGG |
NGG |
2 |
0.2913 |
Tier II |
| 7 |
NC_000083.6 |
17 |
33561660 |
+ |
Myo1f |
NNNTGGGAGATGGTGACAGG |
NGG |
2 |
0.2824 |
Tier II |
| 8 |
NC_000068.7 |
2 |
58668886 |
+ |
Upp2 |
NNNTCACAGATGGTGACAGG |
NAG |
2 |
0.2593 |
Tier II |
| 9 |
NC_000071.6 |
5 |
44478289 |
+ |
Ldb2 |
NNNTGGCAGATGGTGACAGC |
NGG |
2 |
0.2571 |
Tier II |
| 10 |
NC_000076.6 |
10 |
81256778 |
+ |
Matk |
NNNTGGCTGATGGTGACAGG |
NGG |
2 |
0.2571 |
Tier II |
| 11 |
NC_000068.7 |
2 |
161545657 |
- |
Ptprt |
NNNTCACAGATGGTGGCAGG |
NGG |
2 |
0.1923 |
Tier II |
| 12 |
NC_000072.6 |
6 |
28489559 |
+ |
Snd1 |
NNNTCCCAGATGGTGACAGG |
NAG |
2 |
0.1768 |
Tier II |
| 13 |
NC_000068.7 |
2 |
113779490 |
- |
Scg5 |
NNNTGGCAGATGGTGACAGG |
NAG |
2 |
0.1556 |
Tier II |
| 14 |
NC_000075.6 |
9 |
22355548 |
+ |
Anln |
NNNTGGCAGATGGTTACAGG |
NGG |
2 |
0.0857 |
Tier II |
| 15 |
NC_000067.6 |
1 |
86782179 |
- |
Dis3l2 |
NNNTCACAGATGGTGACAGG |
NTG |
2 |
0.039 |
Tier II |
| 16 |
NC_000068.7 |
2 |
155387489 |
+ |
Trp53inp2 |
NNNCCGCAGATGGGGACAGG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000084.6 |
18 |
15030430 |
- |
4933424G05Rik |
NNNTCGGAGATGGAGACAGG |
NGG |
2 |
0.2913 |
Tier III |
| 18 |
NC_000075.6 |
9 |
98803660 |
+ |
E330023G01Rik |
NNNTCACAGATGGTGACAGG |
NAG |
2 |
0.2593 |
Tier III |
Other clones with same target sequence:
(none)
