Construct: sgRNA BRDN0001146810

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAAGTTTGAGCTTTACCTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STYK1 (55359)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75696

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 10633991 + STYK1 NNNGTTTGAGCTTTACCTTG NGG 0 1.0 Tier I
2 NC_000019.10 19 33021572 + RHPN2 NNNCTTTGAGATTTACCTTG NGG 2 0.3971 Tier I
3 NC_000007.14 7 128837475 - FLNC NNNGTTTGAGCTTGGCCTTG NGG 2 0.0 Tier I
4 NC_000013.11 13 31302140 - B3GLCT NNNGTCTAAGCTTTACCTTG NGG 2 0.9091 Tier II
5 NC_000009.12 9 3868721 - GLIS3 NNNGTTTGGGCTTTAACTTG NGG 2 0.6667 Tier II
6 NC_000009.12 9 37526502 - FBXO10 NNNGTCTGGGCTTTACCTTG NGG 2 0.6061 Tier II
7 NC_000008.11 8 52278968 - ST18 NNNATTGGAGCTTTACCTTG NGG 2 0.5294 Tier II
8 NC_000002.12 2 37643630 + CDC42EP3 NNNGTTTCATCTTTACCTTG NGG 2 0.5 Tier II
9 NC_000009.12 9 76431255 - GCNT1 NNNGATTGAGCCTTACCTTG NGG 2 0.4737 Tier II
10 NC_000009.12 9 42023185 - CNTNAP3B NNNGTTTGACCTTTAACTTG NGG 2 0.4 Tier II
11 NC_000005.10 5 111333538 + CAMK4 NNNGTTTTTGCTTTACCTTG NGG 2 0.375 Tier II
12 NC_000022.11 22 31229193 + LIMK2 NNNGTTGCAGCTTTACCTTG NGG 2 0.362 Tier II
13 NC_000016.10 16 3612222 + DNASE1 NNNGTTTGAGCTTCAACTTG NGG 2 0.2857 Tier II
14 NC_000013.11 13 43708373 - ENOX1 NNNGTTTGACCTTTGCCTTG NGG 2 0.26 Tier II
15 NC_000006.12 6 40396997 + LRFN2 NNNGTTTGAACTTTCCCTTG NGG 2 0.2545 Tier II
16 NC_000005.10 5 137627669 - KLHL3 NNNTTTTGAGCTTTGCCTTG NGG 2 0.2364 Tier II
17 NC_000020.11 20 42271109 - PTPRT NNNGTTTGAAGTTTACCTTG NGG 2 0.2333 Tier II
18 NC_000011.10 11 121100675 + TBCEL-TECTA NNNGTTTGAGATTTCCCTTG NGG 2 0.2045 Tier II
19 NC_000009.12 9 83132320 - RASEF NNNGTTAGAGCTTTTCCTTG NGG 2 0.175 Tier II
20 NC_000002.12 2 10573054 - NOL10 NNNTTTTGAGCTTTACCTTC NGG 2 0.1558 Tier II
21 NC_000002.12 2 197843571 - PLCL1 NNNGTTTGAGATTTTCCTTG NGG 2 0.15 Tier II
22 NC_000005.10 5 9229761 - SEMA5A NNNGTTTGTGGTTTACCTTG NGG 2 0.15 Tier II
23 NC_000016.10 16 67390685 - TPPP3 NNNGTTTGAGCTTTCCCCTG NGG 2 0.1169 Tier II
24 NC_000003.12 3 71461094 + FOXP1 NNNGTTTGAGTTTTATCTTG NGG 2 0.0947 Tier II
25 NC_000006.12 6 123033534 - CLVS2 NNNGTTTGAGCTTTAAGTTG NGG 2 0.0588 Tier II
26 NC_000015.10 15 45185404 + SHF NNNGTTTGAGCTTTTCCTAG NGG 2 0.0571 Tier II
27 NC_000019.10 19 50381587 + NR1H2 NNNGTTTGGGCTTTACCTTG NGC 2 0.0148 Tier II
28 NC_000005.10 5 94194038 - KIAA0825 NNNGTTTGACCTTTACCTTG NGT 2 0.0065 Tier II
29 NC_000018.10 18 6773106 + ARHGAP28 NNNGTTTGAGTTTTACCTTG NGT 2 0.005 Tier II
30 NC_000016.10 16 14873187 + NOMO1 NNNCTTTGAGCTTGACCTTG NGG 2 0.0 Tier II
31 NC_000016.10 16 16271687 + NOMO3 NNNCTTTGAGCTTGACCTTG NGG 2 0.0 Tier II
32 NC_000016.10 16 18522986 - NOMO2 NNNCTTTGAGCTTGACCTTG NGG 2 0.0 Tier II
33 NC_000004.12 4 140649074 - TBC1D9 NNNGATTGAGCTTGACCTTG NGG 2 0.0 Tier II
34 NC_000009.12 9 132826518 - AK8 NNNGTTTGAGCCTGACCTTG NGG 2 0.0 Tier II
35 NC_000010.11 10 101755200 + LOC105378458 NNNATTTGAACTTTACCTTG NGG 2 0.84 Tier III
36 NC_000009.12 9 67164231 + CNTNAP3P2 NNNGTTTGACCTTTAACTTG NGG 2 0.4 Tier III
37 NC_000009.12 9 72047303 - LOC101927108 NNNGTTTGACCTTTAACTTG NGG 2 0.4 Tier III
38 NC_000015.10 15 20271670 + RHPN2P1 NNNCTTTGAGATTTACCTTG NGG 2 0.3971 Tier III
39 NC_000018.10 18 38728198 + LOC105372076 NNNCATTGAGCTTTACCTTG NGG 2 0.2647 Tier III
40 NC_000005.10 5 72624280 - TNPO1-DT NNNGTTTGATTTTTACCTTG NGG 2 0.25 Tier III
41 NC_000004.12 4 97224357 - LOC124900737 NNNGTTTGAGTATTACCTTG NGG 2 0.2462 Tier III
42 NC_000010.11 10 5617932 + LINC02677 NNNGTTTGGGTTTTACCTTG NGG 2 0.2051 Tier III
43 NC_000010.11 10 10059418 + LINC02670 NNNGTTTGAGCTCTTCCTTG NGG 2 0.1579 Tier III
44 NC_000016.10 16 67390685 - RNU1-123P NNNGTTTGAGCTTTCCCCTG NGG 2 0.1169 Tier III
45 NC_000016.10 16 60044471 + LINC02141 NNNGTTTGAGCTGTATCTTG NGG 2 0.0803 Tier III
46 NC_000015.10 15 45185404 + LOC124903481 NNNGTTTGAGCTTTTCCTAG NGG 2 0.0571 Tier III
47 NC_000009.12 9 123321491 - LOC105376265 NNNGGTTGAGCTTTACCTTG NGA 2 0.0444 Tier III
48 NC_000017.11 17 36712698 - LOC105371750 NNNGTTTGAGCTTTACCATG NTG 2 0.026 Tier III
49 NC_000005.10 5 94194038 - LOC105379087 NNNGTTTGACCTTTACCTTG NGT 2 0.0065 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 36234900 + Spag1 NNNGTATGAGCTTTAACTTG NGG 2 0.8667 Tier I
2 NC_000072.6 6 131312452 + Styk1 NNNGTTTGAGCCTTACCTCG NGG 2 0.2613 Tier I
3 NC_000073.6 7 27900167 - Zfp607a NNNGTTTGATCTTTACCATG NGG 2 0.5417 Tier II
4 NC_000067.6 1 81288532 + Nyap2 NNNGTTTGAGCATTACTTTG NGG 2 0.3733 Tier II
5 NC_000077.6 11 98819879 + Casc3 NNNGTTTGAGCTATACATTG NGG 2 0.3231 Tier II
6 NC_000075.6 9 119677474 - Scn10a NNNGTTTGAGTCTTACCTTG NGG 2 0.2915 Tier II
7 NC_000080.6 14 48198927 + Peli2 NNNGTTTGACCTTTACCCTG NGG 2 0.1714 Tier II
8 NC_000079.6 13 45531800 - Gmpr NNNGTTTGACCTTTACCTTG NAG 2 0.1037 Tier II
9 NC_000071.6 5 96400402 - Fras1 NNNGTTTGATCTTTACGTTG NGG 2 0.0478 Tier II
10 NC_000082.6 16 6299922 + Rbfox1 NNNGTTTGAGCTTTGCCTTG NGA 2 0.0451 Tier II
11 NC_000071.6 5 28321884 + Rbm33 NNNGTTTTAGCTTTACCTTG NGA 2 0.0434 Tier II
12 NC_000070.6 4 87075225 + Slc24a2 NNNGTTTGAGCTGTACCTTG NGT 2 0.0042 Tier II
13 NC_000071.6 5 39273406 + Gm40293 NNNTTTTGAGCTTTACCTTA NGG 2 0.3409 Tier III
14 NC_000080.6 14 48198927 + Gm6498 NNNGTTTGACCTTTACCCTG NGG 2 0.1714 Tier III
15 NC_000078.6 12 71619059 - 9630002D21Rik NNNGTTTGAGCTTTTCCTTG NTG 2 0.0078 Tier III
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Other clones with same target sequence:

(none)