Construct: sgRNA BRDN0001146812
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATCATGTCGTTATTAACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TGFBR2 (7048)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77468
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
30644747 |
- |
TGFBR2 |
NNNCATGTCGTTATTAACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
62775532 |
- |
EHBP1 |
NNNCATTTCTTTATTAACTG |
NGG |
2 |
0.4643 |
Tier II |
| 3 |
NC_000001.11 |
1 |
71443851 |
+ |
NEGR1 |
NNNCAAGTCGTGATTAACTG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000009.12 |
9 |
18538745 |
+ |
ADAMTSL1 |
NNNCATGTGGTTATTAACAG |
NGG |
2 |
0.1769 |
Tier II |
| 5 |
NC_000003.12 |
3 |
42392250 |
- |
LYZL4 |
NNNCATGTGGTTATGAACTG |
NGG |
2 |
0.031 |
Tier II |
| 6 |
NC_000006.12 |
6 |
70530485 |
- |
FAM135A |
NNNCATGTCGTTATAAACTG |
NTG |
2 |
0.0226 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
68882728 |
- |
Erbb4 |
NNNCATGTTGTTATTAGCTG |
NGG |
2 |
0.1544 |
Tier II |
| 2 |
NC_000075.6 |
9 |
16144572 |
+ |
Fat3 |
NNNCATGTCGTTATCACCTG |
NGG |
2 |
0.0481 |
Tier II |
| 3 |
NC_000071.6 |
5 |
48045935 |
+ |
Slit2 |
NNNCATGTCGTTATTTACTG |
NGT |
2 |
0.0 |
Tier II |
| 4 |
NC_000070.6 |
4 |
4535118 |
- |
Gm33057 |
NNNCTTGTCTTTATTAACTG |
NGG |
2 |
0.2955 |
Tier III |
| 5 |
NC_000078.6 |
12 |
113802482 |
- |
Igh |
NNNCATGTTGTTATTAACTG |
NAG |
2 |
0.2269 |
Tier III |
Other clones with same target sequence:
(none)
