Construct: sgRNA BRDN0001146818

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTGATGAGCCAATGGAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA6 (27330)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75534

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 84164353 - RPS6KA6 NNNGATGAGCCAATGGAAGA NGG 0 1.0 Tier I
2 NC_000013.11 13 102733343 - CCDC168 NNNGATAAGCCAAAGGAAGA NGG 2 0.619 Tier I
3 NC_000002.12 2 213731600 - SPAG16 NNNGAAGAGCCAATGGAAGA NGG 1 0.8667 Tier II
4 NC_000006.12 6 169251130 + THBS2 NNNGATGAGCAAATGGAAGA NGG 1 0.75 Tier II
5 NC_000004.12 4 82793212 + SCD5 NNNGATGAGTCAAAGGAAGA NGG 2 0.5826 Tier II
6 NC_000015.10 15 51719836 - SCG3 NNNGATGAGACAGTGGAAGA NGG 2 0.5652 Tier II
7 NC_000003.12 3 52510676 - STAB1 NNNAATGAGCCAATGGATGA NGG 2 0.45 Tier II
8 NC_000015.10 15 48276164 + SLC12A1 NNNGATGAGCCAATGGAAAT NGG 2 0.4286 Tier II
9 NC_000011.10 11 84111822 - DLG2 NNNGAGGAGCCAATGGAAAA NGG 2 0.4082 Tier II
10 NC_000006.12 6 134283014 + SGK1 NNNGATGAGCCAATGGATGG NGG 2 0.3824 Tier II
11 NC_000007.14 7 47822020 - PKD1L1 NNNGATGATCCAATGGAAAA NGG 2 0.381 Tier II
12 NC_000001.11 1 156655177 - BCAN NNNGATGACCCAAAGGAAGA NGG 2 0.3333 Tier II
13 NC_000007.14 7 87507634 + ABCB1 NNNGAAGAGCCTATGGAAGA NGG 2 0.2889 Tier II
14 NC_000020.11 20 43924146 + TOX2 NNNGATGAGCATATGGAAGA NGG 2 0.25 Tier II
15 NC_000011.10 11 17457467 - ABCC8 NNNGTTGAGCCAAAGGAAGA NGG 2 0.2251 Tier II
16 NC_000007.14 7 104134866 + ORC5 NNNGATGAGAGAATGGAAGA NGG 2 0.2167 Tier II
17 NC_000023.11 X 152930451 + ZNF185 NNNGGTGAGCCAATGGAAGC NGG 2 0.1636 Tier II
18 NC_000012.12 12 29478356 - OVCH1 NNNGATGAGCCAAAGGAAGA NAG 2 0.1605 Tier II
19 NC_000001.11 1 161192900 + ADAMTS4 NNNGAGGAGCCCATGGAAGA NGG 2 0.1504 Tier II
20 NC_000012.12 12 105223504 + APPL2 NNNGAGGAGCCAATGGAAGA NAG 2 0.1481 Tier II
21 NC_000019.10 19 47441123 - SLC8A2 NNNGAGGAGCCACTGGAAGA NGG 2 0.1203 Tier II
22 NC_000002.12 2 62763607 - EHBP1 NNNGTTGAGCTAATGGAAGA NGG 2 0.1119 Tier II
23 NC_000006.12 6 151755396 - ESR1 NNNGATGAGCCCATGGAAGA NAG 2 0.0682 Tier II
24 NC_000012.12 12 120692492 - MLEC NNNGATGAGACAATGGAAGA NGA 2 0.0602 Tier II
25 NC_000002.12 2 68391297 - PLEK NNNTATGAGCCAATGGAAGA NGA 2 0.0253 Tier II
26 NC_000021.9 21 43570204 - HSF2BP NNNGATGAGCTAATGGAAGA NGC 2 0.0068 Tier II
27 NC_000001.11 1 210750734 + KCNH1 NNNAATGAGCCAATGTAAGA NGG 2 0.0 Tier II
28 NC_000014.9 14 93344803 + UNC79 NNNGATGAGCCAAGGGAAGA NTG 2 0.0 Tier II
29 NC_000004.12 4 140930658 - RNF150 NNNGATGAGCCAATGCAGGA NGG 2 0.0 Tier II
30 NC_000021.9 21 9669454 - LOC105379514 NNNGAAGAGCCAATGGAAGA NGG 1 0.8667 Tier III
31 NC_000002.12 2 237947641 - LOC124908001 NNNGATGAGACAAAGGAAGA NGG 2 0.5365 Tier III
32 NC_000002.12 2 67628352 - LOC105374786 NNNGAGGAGACAATGGAAGA NGG 2 0.4952 Tier III
33 NC_000001.11 1 55982947 + LOC105378737 NNNGATGACCCAATGGAAAA NGG 2 0.3846 Tier III
34 NC_000001.11 1 156655177 - BCAN-AS2 NNNGATGACCCAAAGGAAGA NGG 2 0.3333 Tier III
35 NC_000017.11 17 10490528 + MYHAS NNNGATGAGACTATGGAAGA NGG 2 0.2889 Tier III
36 NC_000004.12 4 116844827 + LOC107986306 NNNGATGAGTGAATGGAAGA NGG 2 0.2353 Tier III
37 NC_000009.12 9 1950494 + LOC105375951 NNNGATGAGACAATGGAAGA NAG 2 0.2247 Tier III
38 NC_000023.11 X 152930451 + LOC105373372 NNNGGTGAGCCAATGGAAGC NGG 2 0.1636 Tier III
39 NC_000012.12 12 29478356 - OVCH1-AS1 NNNGATGAGCCAAAGGAAGA NAG 2 0.1605 Tier III
40 NC_000013.11 13 89240543 - LINC00440 NNNGATGTGCCAATGGAAGA NAG 2 0.1111 Tier III
41 NC_000006.12 6 151755396 - LOC107986529 NNNGATGAGCCCATGGAAGA NAG 2 0.0682 Tier III
42 NC_000003.12 3 104828803 - LOC107986108 NNNGATGAGCCAAAGGAAGA NGT 2 0.01 Tier III
43 NC_000001.11 1 210750734 + LOC105372901 NNNAATGAGCCAATGTAAGA NGG 2 0.0 Tier III
44 NC_000024.10 Y 19044154 - TTTY14 NNNGATGAGCCAAGGGAAGA NAG 2 0.0 Tier III
45 NC_000017.11 17 72075530 - SOX9-AS1 NNNGATGAGCCACTGCAAGA NGG 2 0.0 Tier III
46 NC_000017.11 17 72075530 - LINC02097 NNNGATGAGCCACTGCAAGA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 111446256 - Rps6ka6 NNNGATGAGCCAATGGAAGA NGG 0 1.0 Tier I
2 NC_000077.6 11 70219199 + Slc16a13 NNNGATCAGCCACTGGAAGA NGG 2 0.1447 Tier I
3 NC_000082.6 16 4039221 - Dnase1 NNNGATCAGCCACTGGAAGA NGG 2 0.1447 Tier I
4 NC_000073.6 7 46943836 - Uevld NNNGATGAGCCAAGGGACGA NGG 2 0.0 Tier I
5 NC_000075.6 9 114016214 - Fbxl2 NNNAATGAGCAAATGGAAGA NGG 2 0.675 Tier II
6 NC_000076.6 10 107804606 + Otogl NNNGATGAGAAAATGGAAGA NGG 2 0.65 Tier II
7 NC_000078.6 12 103393449 + Otub2 NNNGGTGAGACAATGGAAGA NGG 2 0.624 Tier II
8 NC_000075.6 9 102019430 + Ephb1 NNNGATGAGCAAATGGAACA NGG 2 0.3362 Tier II
9 NC_000069.6 3 142862187 - Pkn2 NNNGATGAGCTAATGAAAGA NGG 2 0.3077 Tier II
10 NC_000068.7 2 53098345 - Fmnl2 NNNGATGCGCCAAAGGAAGA NGG 2 0.2653 Tier II
11 NC_000077.6 11 86700194 + Cltc NNNGATAAGCCAATGGAAGA NAG 2 0.2593 Tier II
12 NC_000085.6 19 46483204 + Sufu NNNGATGAGGCAAAGGAAGA NGG 2 0.2407 Tier II
13 NC_000074.6 8 118782120 - Cdh13 NNNCATGAGGCAATGGAAGA NGG 2 0.2059 Tier II
14 NC_000073.6 7 25099839 + Pou2f2 NNNGATGGGCCAATGGAAGA NAG 2 0.1901 Tier II
15 NC_000080.6 14 10576452 + Fhit NNNTCTGAGCCAATGGAAGA NGG 2 0.1818 Tier II
16 NC_000068.7 2 130350465 - Ebf4 NNNGATGAGTCAATGGGAGA NGG 2 0.1661 Tier II
17 NC_000071.6 5 36175305 + Sorcs2 NNNGGTGAGCCACTGGAAGA NGG 2 0.1516 Tier II
18 NC_000070.6 4 126726270 + Tfap2e NNNGATGAGCCAGTGGAAGC NGG 2 0.1482 Tier II
19 NC_000068.7 2 121023283 - Epb42 NNNCATGAGCCACTGGAAGA NGG 2 0.1115 Tier II
20 NC_000075.6 9 111149582 + Lrrfip2 NNNGATGAGCCAATAGAAGA NGA 2 0.0654 Tier II
21 NC_000085.6 19 24415166 - Pip5k1b NNNGATGAGCCACTGGAAGC NGG 2 0.0478 Tier II
22 NC_000079.6 13 16990102 - Sugct NNNGATGAACCAATGGAAGA NGA 2 0.0446 Tier II
23 NC_000072.6 6 146467401 - Itpr2 NNNGATGAACCAATGCAAGA NGG 2 0.0 Tier II
24 NC_000084.6 18 63170963 + Piezo2 NNNGATGAGCCAATGCAAGA NAG 2 0.0 Tier II
25 NC_000073.6 7 138505562 + Gm30751 NNNGCTGAGCCAATGGAAGA NAG 2 0.1296 Tier III
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Other clones with same target sequence:

(none)