Construct: sgRNA BRDN0001146819

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAAGAACTGAACTAAATGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KDR (3791)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77755

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 55114165 - KDR NNNGAACTGAACTAAATGTG NGG 0 1.0 Tier I
2 NC_000012.12 12 120179117 - GCN1 NNNGGACAGAACTAAATGTG NGG 2 0.576 Tier II
3 NC_000005.10 5 161851167 + GABRA1 NNNGAACTGAACAAAACGTG NGG 2 0.4887 Tier II
4 NC_000003.12 3 29451039 - RBMS3 NNNGCATTGAACTAAATGTG NGG 2 0.4063 Tier II
5 NC_000018.10 18 23861944 + LAMA3 NNNGAGCTGAACTAAAAGTG NGG 2 0.381 Tier II
6 NC_000002.12 2 153126209 + GALNT13 NNNGAACTCAACTAAATGTT NGG 2 0.3769 Tier II
7 NC_000020.11 20 58433838 + VAPB NNNGAACTTAACTAGATGTG NGG 2 0.3467 Tier II
8 NC_000001.11 1 180247536 - LHX4 NNNGAACTGAGCTAAATGTC NGG 2 0.2786 Tier II
9 NC_000021.9 21 41219905 - BACE2 NNNGAACTGAACTAAATTAG NGG 2 0.1905 Tier II
10 NC_000018.10 18 59634013 + CCBE1 NNNGAACTGCATTAAATGTG NGG 2 0.1795 Tier II
11 NC_000006.12 6 145515298 + EPM2A NNNGAACTGAAATAAATGGG NGG 2 0.1786 Tier II
12 NC_000002.12 2 54972248 - RTN4 NNNGTACTGAACTAAATCTG NGG 2 0.1732 Tier II
13 NC_000009.12 9 95297127 + FANCC NNNGAACTGAACCATATGTG NGG 2 0.1579 Tier II
14 NC_000023.11 X 135284085 - ZNF75D NNNGAACTGAACCAAGTGTG NGG 2 0.1518 Tier II
15 NC_000005.10 5 16904548 + MYO10 NNNGAACTGAACTGAATGAG NGG 2 0.1333 Tier II
16 NC_000019.10 19 53176715 + ZNF665 NNNGAACTGAACTGAATGGG NGG 2 0.1167 Tier II
17 NC_000002.12 2 200425085 + SPATS2L NNNGAACTGAACCAAATGTG NGA 2 0.0548 Tier II
18 NC_000020.11 20 14776045 + MACROD2 NNNGAACTGAACCAAATGTG NGT 2 0.0127 Tier II
19 NC_000010.11 10 71680422 - CDH23 NNNGAACTGAACTAACTGTG NGG 1 0.0 Tier II
20 NC_000020.11 20 10897878 + LOC101929413 NNNGAACTGAACCAAATGTT NGG 2 0.5526 Tier III
21 NC_000017.11 17 15150032 + LOC107984976 NNNGAACTGAAAGAAATGTG NGG 2 0.1863 Tier III
22 NC_000014.9 14 24467979 - LOC101927045 NNNGAAATGAACTCAATGTG NGG 2 0.1607 Tier III
23 NC_000007.14 7 134630191 + LOC124901750 NNNGAACTGAACTGAATGAG NGG 2 0.1333 Tier III
24 NC_000003.12 3 59713379 + CFAP20DC-DT NNNGAAGTGAACTAAATGTG NAG 2 0.122 Tier III
25 NC_000002.12 2 200425085 + LOC101927741 NNNGAACTGAACCAAATGTG NGA 2 0.0548 Tier III
26 NC_000014.9 14 48984631 + LOC105378178 NNNGAACTGAACTAAGTGTG NAG 2 0.0499 Tier III
27 NC_000008.11 8 128037796 - PVT1 NNNGCACTGAACTAAATGTG NTG 2 0.0195 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 56571424 - Safb2 NNNGAACTGAACTACATGGG NGG 2 0.0682 Tier I
2 NC_000071.6 5 24107309 + Klhl7 NNNGAACTGAACAAAATGTA NGG 2 0.649 Tier II
3 NC_000070.6 4 19522923 - Cpne3 NNNGAATTGAACTTAATGTG NGG 2 0.4333 Tier II
4 NC_000071.6 5 27044399 - Dpp6 NNNGAACTGAACCAAATCTG NGG 2 0.3759 Tier II
5 NC_000086.7 X 83606203 - Dmd NNNGAACTGAACCAAATCTG NGG 2 0.3759 Tier II
6 NC_000073.6 7 64339700 - Mtmr10 NNNGAGCTGAAGTAAATGTG NGG 2 0.3175 Tier II
7 NC_000077.6 11 6318543 - Ogdh NNNAAACTGAACTAAATGAG NGG 2 0.2571 Tier II
8 NC_000083.6 17 10425233 + Pacrg NNNGAAGTGAACTTAATGTG NGG 2 0.251 Tier II
9 NC_000078.6 12 116301771 + Esyt2 NNNGGACTGAACTAAATGTG NAG 2 0.1867 Tier II
10 NC_000068.7 2 153034181 + Xkr7 NNNGAACTGAACTAGATGGG NGG 2 0.1625 Tier II
11 NC_000068.7 2 156865097 - Rab5if NNNGTACTGAACTAAAGGTG NGG 2 0.0428 Tier II
12 NC_000068.7 2 135988545 + Plcb4 NNNGAACTGAACTATGTGTG NGG 2 0.0385 Tier II
13 NC_000071.6 5 92309115 + Sdad1 NNNGAACTGAAATAAATGTG NTG 2 0.0278 Tier II
14 NC_000071.6 5 115505287 + Pxn NNNGAACTGAACTGACTGTG NGG 2 0.0 Tier II
15 NC_000083.6 17 5240502 + Arid1b NNNGAACTGCACTAATTGTG NGG 2 0.0 Tier II
16 NC_000068.7 2 34024908 + C230014O12Rik NNNGAACTGAATTAAATGTA NGG 2 0.5048 Tier III
17 NC_000073.6 7 45299368 + Gm39006 NNNGGACTGAACTAAATGTC NGG 2 0.3086 Tier III
18 NC_000084.6 18 46376375 + Gm4107 NNNGAAGTGAACTATATGTG NGG 2 0.0941 Tier III
19 NC_000084.6 18 46376375 + Gm32339 NNNGAAGTGAACTATATGTG NGG 2 0.0941 Tier III
20 NC_000082.6 16 5431385 - Gm41414 NNNGAACTGAGCTAAATGTG NGA 2 0.0451 Tier III
Download CSV

Other clones with same target sequence:

(none)