Construct: sgRNA BRDN0001146823
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTGATGCTCACCTCCCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SBK1 (388228)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75798
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
28319056 |
- |
SBK1 |
NNNTGATGCTCACCTCCCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
1063859 |
+ |
ABCA7 |
NNNTGATGCTCACCTCCCAT |
NGG |
1 |
0.7143 |
Tier I |
| 3 |
NC_000016.10 |
16 |
24193836 |
+ |
PRKCB |
NNNTGATGTTCACCTCCCGA |
NGG |
2 |
0.4922 |
Tier II |
| 4 |
NC_000004.12 |
4 |
182139228 |
- |
TENM3 |
NNNTGATACTCTCCTCCCGT |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000020.11 |
20 |
41352511 |
- |
LPIN3 |
NNNTGCTGCTCACCTCCTGT |
NGG |
2 |
0.2922 |
Tier II |
| 6 |
NC_000010.11 |
10 |
6570520 |
- |
PRKCQ |
NNNTGAGGCTCACCTCCCCT |
NGG |
2 |
0.2637 |
Tier II |
| 7 |
NC_000011.10 |
11 |
74296194 |
- |
P4HA3 |
NNNTGATGCTCACCTCAAGT |
NGG |
2 |
0.2513 |
Tier II |
| 8 |
NC_000022.11 |
22 |
23126659 |
- |
RSPH14 |
NNNTGGTGCTTACCTCCCGT |
NGG |
2 |
0.2198 |
Tier II |
| 9 |
NC_000020.11 |
20 |
46373367 |
+ |
ELMO2 |
NNNTGATGGTCTCCTCCCGT |
NGG |
2 |
0.2063 |
Tier II |
| 10 |
NC_000023.11 |
X |
54809220 |
- |
MAGED2 |
NNNTGATCCTCACCTTCCGT |
NGG |
2 |
0.1893 |
Tier II |
| 11 |
NC_000017.11 |
17 |
58272710 |
- |
MPO |
NNNTGATGCTCACCTCCCCT |
NAG |
2 |
0.1162 |
Tier II |
| 12 |
NC_000001.11 |
1 |
10723055 |
- |
CASZ1 |
NNNTGATGCGCACCTCCCGT |
NTG |
2 |
0.0195 |
Tier II |
| 13 |
NC_000004.12 |
4 |
182139228 |
- |
TENM3-AS1 |
NNNTGATACTCTCCTCCCGT |
NGG |
2 |
0.3333 |
Tier III |
| 14 |
NC_000001.11 |
1 |
151129831 |
+ |
LOC124904418 |
NNNTGATGCTATCCTCCCGT |
NGG |
2 |
0.25 |
Tier III |
| 15 |
NC_000020.11 |
20 |
46373367 |
+ |
LOC124904917 |
NNNTGATGGTCTCCTCCCGT |
NGG |
2 |
0.2063 |
Tier III |
| 16 |
NC_000007.14 |
7 |
10695736 |
- |
MGC4859 |
NNNTGCTGCTCACCTTCCGT |
NGG |
2 |
0.1399 |
Tier III |
| 17 |
NC_000007.14 |
7 |
10695736 |
- |
LOC124900231 |
NNNTGCTGCTCACCTTCCGT |
NGG |
2 |
0.1399 |
Tier III |
| 18 |
NC_000004.12 |
4 |
40308325 |
- |
LINC02265 |
NNNTGCTGCTCACCTGCCGT |
NGG |
2 |
0.0699 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
103683086 |
- |
Gosr2 |
NNNTGAAGCTCACCTCCTGT |
NGG |
2 |
0.5625 |
Tier II |
| 2 |
NC_000079.6 |
13 |
43104914 |
+ |
Phactr1 |
NNNGGATGCTCACCTCCCTT |
NGG |
2 |
0.4167 |
Tier II |
| 3 |
NC_000075.6 |
9 |
21673784 |
+ |
Smarca4 |
NNNTGATGCTCACTTGCCGT |
NGG |
2 |
0.1128 |
Tier II |
| 4 |
NC_000074.6 |
8 |
92680524 |
- |
Irx6 |
NNNTGATGCTCACCTCCCAC |
NGG |
2 |
0.0649 |
Tier II |
Other clones with same target sequence:
(none)
