Construct: sgRNA BRDN0001146826
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATCGTGAAGAACTACACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TKFC (26007)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75784
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
61339284 |
+ |
TKFC |
NNNCGTGAAGAACTACACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
45815611 |
- |
UBE2C |
NNNCGTGAGGAACTTCACTG |
NGG |
2 |
0.1333 |
Tier I |
| 3 |
NC_000011.10 |
11 |
113335033 |
+ |
TTC12 |
NNNCCTGAAGAACTACAGTG |
NGG |
2 |
0.1048 |
Tier I |
| 4 |
NC_000006.12 |
6 |
46554888 |
- |
CYP39A1 |
NNNGATGAAGAACTACACTG |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000001.11 |
1 |
220553801 |
+ |
MARK1 |
NNNCCTGAAGATCTACACTG |
NGG |
2 |
0.2619 |
Tier II |
| 6 |
NC_000003.12 |
3 |
185517564 |
+ |
LIPH |
NNNCTTGAAGAACTACAATG |
NGG |
2 |
0.1615 |
Tier II |
| 7 |
NC_000010.11 |
10 |
99817563 |
+ |
ABCC2 |
NNNGGTGAAGAACTACACAG |
NGG |
2 |
0.1429 |
Tier II |
| 8 |
NC_000011.10 |
11 |
129163417 |
+ |
ARHGAP32 |
NNNCGTGAAAAACTACACTG |
NTG |
2 |
0.0364 |
Tier II |
| 9 |
NC_000007.14 |
7 |
81724327 |
+ |
HGF |
NNNCGTGAAGAATGACACTG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000006.12 |
6 |
4511666 |
+ |
LOC105374894 |
NNNCGTGGAGAACAACACTG |
NGG |
2 |
0.454 |
Tier III |
| 11 |
NC_000018.10 |
18 |
60525806 |
- |
LOC100421385 |
NNNTGTGAAGAACTACACTG |
NGA |
2 |
0.0556 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
10599184 |
- |
Tkfc |
NNNTGTGAAGAACTACACTG |
NGG |
1 |
0.8 |
Tier I |
| 2 |
NC_000084.6 |
18 |
57907106 |
- |
Slc12a2 |
NNNCGTACAGAACTACACTG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000067.6 |
1 |
11736285 |
- |
A830018L16Rik |
NNNCGTAAAGAAATACACTG |
NGG |
2 |
0.3846 |
Tier II |
| 4 |
NC_000068.7 |
2 |
165615686 |
+ |
Eya2 |
NNNCGTGAAAAACTATACTG |
NGG |
2 |
0.2872 |
Tier II |
| 5 |
NC_000072.6 |
6 |
4512980 |
+ |
Col1a2 |
NNNGGTTAAGAACTACACTG |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000073.6 |
7 |
126886248 |
- |
Tmem219 |
NNNAGTGAAGAACTAGACTG |
NGG |
2 |
0.1296 |
Tier II |
| 7 |
NC_000073.6 |
7 |
126886248 |
- |
Taok2 |
NNNAGTGAAGAACTAGACTG |
NGG |
2 |
0.1296 |
Tier II |
| 8 |
NC_000086.7 |
X |
10182400 |
- |
Rpgr |
NNNCATGAAGAAGTACACTG |
NGG |
2 |
0.1182 |
Tier II |
| 9 |
NC_000067.6 |
1 |
178843195 |
- |
Kif26b |
NNNCGTGAGGAACTACACTG |
NGT |
2 |
0.0108 |
Tier II |
| 10 |
NC_000067.6 |
1 |
155721829 |
+ |
Lhx4 |
NNNCGTGAAGAACGACTCTG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000074.6 |
8 |
83601321 |
+ |
Gm42029 |
NNNCCTGAAGAACAACACTG |
NGG |
2 |
0.4864 |
Tier III |
Other clones with same target sequence:
(none)
