Construct: sgRNA BRDN0001146827
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCACTTGGCGCTCACAGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKD (8527)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77257
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
233436354 |
- |
DGKD |
NNNACTTGGCGCTCACAGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
42148647 |
+ |
PTPRT |
NNNACCTGGCACTCACAGGT |
NGG |
2 |
0.9091 |
Tier II |
| 3 |
NC_000008.11 |
8 |
138748309 |
- |
COL22A1 |
NNNACTAGGAGCTCACAGGT |
NGG |
2 |
0.7583 |
Tier II |
| 4 |
NC_000006.12 |
6 |
138486472 |
- |
NHSL1 |
NNNTCATGGCGCTCACAGGT |
NGG |
2 |
0.5515 |
Tier II |
| 5 |
NC_000001.11 |
1 |
213663777 |
+ |
RPS6KC1 |
NNNACTTGGCACTCACAGCT |
NGG |
2 |
0.4483 |
Tier II |
| 6 |
NC_000008.11 |
8 |
11563535 |
+ |
BLK |
NNNACTTGGCCCCCACAGGT |
NGG |
2 |
0.3383 |
Tier II |
| 7 |
NC_000002.12 |
2 |
191622968 |
+ |
LOC124905959 |
NNNAATTAGCGCTCACAGGT |
NGG |
2 |
0.5714 |
Tier III |
| 8 |
NC_000016.10 |
16 |
75799557 |
- |
LOC105371348 |
NNNAATTGGCTCTCACAGGT |
NGG |
2 |
0.2198 |
Tier III |
| 9 |
NC_000002.12 |
2 |
175666501 |
- |
LOC107985962 |
NNNACTTGGCGCTCTCAGCT |
NGG |
2 |
0.0897 |
Tier III |
| 10 |
NC_000008.11 |
8 |
46975390 |
- |
MAPK6P4 |
NNNACTTGGCTCTCACGGGT |
NGG |
2 |
0.0679 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
87916845 |
- |
Dgkd |
NNNACTTGGCACTCACAGGC |
NGG |
2 |
0.0909 |
Tier I |
| 2 |
NC_000070.6 |
4 |
123356862 |
+ |
Macf1 |
NNNACTTAGAGCTCACAGGT |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000072.6 |
6 |
88421973 |
+ |
Eefsec |
NNNACCTGGAGCTCACAGGT |
NGG |
2 |
0.7879 |
Tier II |
| 4 |
NC_000078.6 |
12 |
4772817 |
- |
Pfn4 |
NNNACTTGGTGCTCACAGGA |
NGG |
2 |
0.5294 |
Tier II |
| 5 |
NC_000081.6 |
15 |
101286932 |
+ |
Atg101 |
NNNACTTGGTTCTCACAGGT |
NGG |
2 |
0.362 |
Tier II |
| 6 |
NC_000075.6 |
9 |
65576546 |
- |
Plekho2 |
NNNACTTGGTGCTCAGAGGT |
NGG |
2 |
0.1448 |
Tier II |
| 7 |
NC_000071.6 |
5 |
37551535 |
- |
Stk32b |
NNNACTTGGAGCTCACTGGT |
NGG |
2 |
0.1156 |
Tier II |
| 8 |
NC_000068.7 |
2 |
28725639 |
+ |
Ak8 |
NNNCCTTGGCGCTCACAGGT |
NAG |
2 |
0.0915 |
Tier II |
| 9 |
NC_000071.6 |
5 |
114865715 |
+ |
4930519G04Rik |
NNNACTTGGCCCTCACTGGT |
NGG |
2 |
0.0571 |
Tier II |
| 10 |
NC_000082.6 |
16 |
6342305 |
+ |
Rbfox1 |
NNNACTTGGAGCTCACAGGT |
NTG |
2 |
0.0338 |
Tier II |
| 11 |
NC_000068.7 |
2 |
180845104 |
- |
Gm14344 |
NNNACTTGACCCTCACAGGT |
NGG |
2 |
0.2755 |
Tier III |
| 12 |
NC_000068.7 |
2 |
180845104 |
- |
Gm14342 |
NNNACTTGACCCTCACAGGT |
NGG |
2 |
0.2755 |
Tier III |
| 13 |
NC_000074.6 |
8 |
87119731 |
- |
Gm27246 |
NNNGCTTGGGGCTCACAGGT |
NGG |
2 |
0.2431 |
Tier III |
| 14 |
NC_000075.6 |
9 |
61123646 |
+ |
Gm33914 |
NNNACTTGGAGCTCAGAGGT |
NGG |
2 |
0.1333 |
Tier III |
Other clones with same target sequence:
(none)
