Construct: sgRNA BRDN0001146833
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTCACTAAACTTGTATTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAGI1 (9223)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78067
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000072.6 |
6 |
93745904 |
+ |
Magi1 |
NNNTCACTAAACTTGTATTA |
NGG |
1 |
0.9375 |
Tier I |
2 |
NC_000085.6 |
19 |
13027419 |
- |
Olfr1453 |
NNNTCACAAAAATTGTATTG |
NGG |
2 |
0.5714 |
Tier I |
3 |
NC_000071.6 |
5 |
45613792 |
+ |
Fam184b |
NNNTCACCAAACTTGTATTA |
NGG |
2 |
0.9375 |
Tier II |
4 |
NC_000072.6 |
6 |
54852284 |
+ |
Znrf2 |
NNNTCACTAAATTTGTATTA |
NGG |
2 |
0.5048 |
Tier II |
5 |
NC_000071.6 |
5 |
89219494 |
+ |
Slc4a4 |
NNNTCAATAAGCTTGTATTG |
NGG |
2 |
0.4875 |
Tier II |
6 |
NC_000086.7 |
X |
141346795 |
+ |
Col4a6 |
NNNTCACTCAACTTGTAATG |
NGG |
2 |
0.381 |
Tier II |
7 |
NC_000069.6 |
3 |
153438454 |
- |
St6galnac3 |
NNNTCACAAAACTTGTATGG |
NGG |
2 |
0.2 |
Tier II |
8 |
NC_000085.6 |
19 |
56331302 |
- |
Nrap |
NNNTCACTAAAATTGTTTTG |
NGG |
2 |
0.0952 |
Tier II |
9 |
NC_000073.6 |
7 |
121099727 |
- |
Otoa |
NNNTCACTAAACTTGCTTTG |
NGG |
2 |
0.0889 |
Tier II |
10 |
NC_000075.6 |
9 |
105862298 |
+ |
Col6a5 |
NNNTCACTAAAGTTTTATTG |
NGG |
2 |
0.0635 |
Tier II |
11 |
NC_000083.6 |
17 |
90461825 |
+ |
Nrxn1 |
NNNTCACCAAACTTGTATTG |
NTG |
2 |
0.039 |
Tier II |
12 |
NC_000077.6 |
11 |
81457139 |
- |
Asic2 |
NNNTCATTAAACTTGTATTG |
NTG |
2 |
0.0317 |
Tier II |
13 |
NC_000077.6 |
11 |
29768718 |
- |
Eml6 |
NNNTCTCTAAACTTGTATTG |
NTG |
2 |
0.0278 |
Tier II |
14 |
NC_000067.6 |
1 |
137921041 |
- |
Gm4258 |
NNNTGACTAAACTTGTATTC |
NGG |
2 |
0.2571 |
Tier III |
15 |
NC_000081.6 |
15 |
61329557 |
- |
Gm29904 |
NNNTCACTAAACTTCTACTG |
NGG |
2 |
0.1169 |
Tier III |
Other clones with same target sequence:
(none)