Construct: sgRNA BRDN0001146837
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGACGAGCAGCGAACAGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AURKB (9212)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77619
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
8206755 |
- |
AURKB |
NNNCGAGCAGCGAACAGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
72421675 |
- |
CLPB |
NNNTGAGCAGAGAACAGCCA |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000006.12 |
6 |
41941667 |
+ |
CCND3 |
NNNCGGGCAGCGAACAGGCA |
NGG |
2 |
0.0952 |
Tier II |
| 4 |
NC_000015.10 |
15 |
61578758 |
+ |
LOC107984782 |
NNNCGGGGAGCGAACAGCCA |
NGG |
2 |
0.4592 |
Tier III |
| 5 |
NC_000002.12 |
2 |
113382908 |
- |
LINC02966 |
NNNCTAGCAGAGAACAGCCA |
NGG |
2 |
0.225 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
6997648 |
+ |
Aurkc |
NNNCCAGCAGCGTACAGCCA |
NGG |
2 |
0.2357 |
Tier I |
| 2 |
NC_000086.7 |
X |
74023471 |
- |
Irak1 |
NNNCGAGCAGCGCACAGCCA |
NTG |
2 |
0.0082 |
Tier I |
| 3 |
NC_000068.7 |
2 |
33743585 |
+ |
Mvb12b |
NNNCAAGCAGCGGACAGCCA |
NGG |
2 |
0.5652 |
Tier II |
| 4 |
NC_000085.6 |
19 |
40638821 |
- |
Entpd1 |
NNNCGAGCAGAGAACACCCA |
NGG |
2 |
0.1765 |
Tier II |
| 5 |
NC_000068.7 |
2 |
33743585 |
+ |
Gm52507 |
NNNCAAGCAGCGGACAGCCA |
NGG |
2 |
0.5652 |
Tier III |
| 6 |
NC_000078.6 |
12 |
23091817 |
- |
Gm9293 |
NNNCGAGAAGCGAACAGACA |
NGG |
2 |
0.35 |
Tier III |
| 7 |
NC_000073.6 |
7 |
11234541 |
+ |
Gm39481 |
NNNAGAGCAGCGTACAGCCA |
NGG |
2 |
0.2526 |
Tier III |
| 8 |
NC_000073.6 |
7 |
10813499 |
+ |
Gm7203 |
NNNCCAGCAGCGTACAGCCA |
NGG |
2 |
0.2357 |
Tier III |
| 9 |
NC_000073.6 |
7 |
11084002 |
+ |
Gm7151 |
NNNCCAGCAGCGTACAGCCA |
NGG |
2 |
0.2357 |
Tier III |
| 10 |
NC_000069.6 |
3 |
94589598 |
+ |
Gm15235 |
NNNCGAGCAGCTAAAAGCCA |
NGG |
2 |
0.0855 |
Tier III |
Other clones with same target sequence:
(none)
