Construct: sgRNA BRDN0001146838
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGATAGTTCAACCCTCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STRADB (55437)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75495
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
201475703 |
- |
STRADB |
NNNATAGTTCAACCCTCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
38749685 |
+ |
SRSF7 |
NNNATAGTTCAACCCTCACT |
NGG |
2 |
0.4 |
Tier I |
| 3 |
NC_000001.11 |
1 |
48436475 |
- |
SPATA6 |
NNNATAGTTCAACCCTCTCA |
NAG |
1 |
0.2593 |
Tier II |
| 4 |
NC_000006.12 |
6 |
36759044 |
- |
CPNE5 |
NNNATAGTTCAACACCCTCA |
NGG |
2 |
0.2333 |
Tier II |
| 5 |
NC_000010.11 |
10 |
10658282 |
- |
CELF2 |
NNNATATTTCAACACTCTCA |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000012.12 |
12 |
40797016 |
+ |
CNTN1 |
NNNTTAGTTCAACCCTCTCA |
NAG |
2 |
0.165 |
Tier II |
| 7 |
NC_000006.12 |
6 |
79940687 |
+ |
ELOVL4 |
NNNGTAGTTCAACCCTCTCA |
NAG |
2 |
0.162 |
Tier II |
| 8 |
NC_000003.12 |
3 |
114987233 |
- |
ZBTB20 |
NNNATATTTCAACCATCTCA |
NGG |
2 |
0.127 |
Tier II |
| 9 |
NC_000008.11 |
8 |
31935855 |
- |
NRG1 |
NNNATAGTCCAAGCCTCTCA |
NGG |
2 |
0.1259 |
Tier II |
| 10 |
NC_000008.11 |
8 |
28623909 |
- |
EXTL3 |
NNNATAGTTCAACCTTCTCA |
NAG |
2 |
0.0173 |
Tier II |
| 11 |
NC_000001.11 |
1 |
48436475 |
- |
LOC644462 |
NNNATAGTTCAACCCTCTCA |
NAG |
1 |
0.2593 |
Tier III |
| 12 |
NC_000003.12 |
3 |
150118290 |
+ |
LOC105374313 |
NNNATAGTTCAACACTCTCA |
NAG |
2 |
0.0907 |
Tier III |
| 13 |
NC_000007.14 |
7 |
150513309 |
+ |
STRADBP1 |
NNNATAGTTCAATCCTCTCA |
NTG |
2 |
0.0273 |
Tier III |
| 14 |
NC_000017.11 |
17 |
35910402 |
+ |
LRRC37A8P |
NNNATAGTTCAACCTTCTCA |
NGT |
2 |
0.0011 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
31302490 |
+ |
Prdm10 |
NNNATAGTTCAACTCTCTCA |
NGG |
1 |
0.7333 |
Tier II |
| 2 |
NC_000080.6 |
14 |
71268495 |
+ |
Gm4251 |
NNNATGGTTTAACCCTCTCA |
NGG |
2 |
0.6723 |
Tier III |
| 3 |
NC_000080.6 |
14 |
44925543 |
- |
Gm16976 |
NNNATAGTTCAACCCTTCCA |
NGG |
2 |
0.2 |
Tier III |
Other clones with same target sequence:
(none)
