Construct: sgRNA BRDN0001146840
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCACCCGGAGAATTCAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LTK (4058)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77599
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
41512989 |
+ |
LTK |
NNNACCCGGAGAATTCAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
48545744 |
+ |
AGBL4 |
NNNACCAGGAGAATTCAGCT |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000012.12 |
12 |
95974668 |
+ |
HAL |
NNNACCCAGAGAATTCAGTG |
NGG |
2 |
0.4615 |
Tier II |
| 4 |
NC_000021.9 |
21 |
34886058 |
- |
RUNX1 |
NNNAGCCGGAGACTTCAGCG |
NGG |
2 |
0.1263 |
Tier II |
| 5 |
NC_000018.10 |
18 |
48619396 |
- |
CTIF |
NNNACCCTGAGAAGTCAGCG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000016.10 |
16 |
25103598 |
+ |
LCMT1-AS1 |
NNNACTCTGAGAATTCAGCG |
NGG |
2 |
0.5804 |
Tier III |
| 7 |
NC_000008.11 |
8 |
129447115 |
+ |
CCDC26 |
NNNACCTGGAGAATTCAGAG |
NGG |
2 |
0.3482 |
Tier III |
| 8 |
NC_000009.12 |
9 |
24071186 |
- |
LOC124902327 |
NNNACCCGGAGAATGCAGCA |
NGG |
2 |
0.0469 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
14931246 |
- |
Arhgef10 |
NNNACACGAAGAATTCAGCG |
NGG |
2 |
0.5969 |
Tier II |
| 2 |
NC_000079.6 |
13 |
56881454 |
+ |
Trpc7 |
NNNACCCTGAGAATTCAGCA |
NGG |
2 |
0.5859 |
Tier II |
| 3 |
NC_000067.6 |
1 |
165237101 |
- |
Tiprl |
NNNACCCGGAGACTTCAGGG |
NGG |
2 |
0.0263 |
Tier II |
Other clones with same target sequence:
(none)
