Construct: sgRNA BRDN0001146855
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCAACGAATGACTCCAACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRIM24 (8805)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77886
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
138504291 |
- |
TRIM24 |
NNNAACGAATGACTCCAACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
236457910 |
- |
IQCA1 |
NNNAACAAATGACACCAACT |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000003.12 |
3 |
130718372 |
+ |
PIK3R4 |
NNNAAAGACTGACTCCAACT |
NGG |
2 |
0.5306 |
Tier II |
| 4 |
NC_000018.10 |
18 |
28072883 |
- |
CDH2 |
NNNAACCCATGACTCCAACT |
NGG |
2 |
0.2946 |
Tier II |
| 5 |
NC_000009.12 |
9 |
98973767 |
- |
COL15A1 |
NNNAAGGAATGACTCCAACA |
NGG |
2 |
0.2813 |
Tier II |
| 6 |
NC_000004.12 |
4 |
3336209 |
- |
RGS12 |
NNNAAGGAATTACTCCAACT |
NGG |
2 |
0.1923 |
Tier II |
| 7 |
NC_000002.12 |
2 |
41938001 |
+ |
LINC02898 |
NNNAATGAATGGCTCCAACT |
NGG |
2 |
0.6706 |
Tier III |
| 8 |
NC_000002.12 |
2 |
41938001 |
+ |
LOC124905996 |
NNNAATGAATGGCTCCAACT |
NGG |
2 |
0.6706 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
8371830 |
+ |
Ssxb9 |
NNNAAAGAATGATTCCAACT |
NGG |
2 |
0.65 |
Tier I |
| 2 |
NC_000086.7 |
X |
8586970 |
- |
Ssxb3 |
NNNAAAGAATGATTCCAACT |
NGG |
2 |
0.65 |
Tier I |
| 3 |
NC_000072.6 |
6 |
37903534 |
- |
Trim24 |
NNNATCGAATGACTCCAACT |
NGG |
1 |
0.3636 |
Tier I |
| 4 |
NC_000076.6 |
10 |
76620143 |
+ |
Col6a2 |
NNNAATGAATGACTCCAACA |
NGG |
2 |
0.5223 |
Tier II |
| 5 |
NC_000083.6 |
17 |
11375503 |
+ |
Prkn |
NNNAAGGAAAGACTCCAACT |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000083.6 |
17 |
65999926 |
- |
Ankrd12 |
NNNAAGGAATGACTCCAACT |
NTG |
2 |
0.0195 |
Tier II |
Other clones with same target sequence:
(none)
