Construct: sgRNA BRDN0001146856

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TATCTTCCCAGAGTTACTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2D (817)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77187

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 113552091 - CAMK2D NNNCTTCCCAGAGTTACTGG NGG 0 1.0 Tier I
2 NC_000004.12 4 151662672 + FHIP1A NNNCTTCCCAGAGTTCCTGA NGG 2 0.0 Tier I
3 NC_000016.10 16 6458876 + RBFOX1 NNNTTTCCCAGAATTACTGG NGG 2 0.7385 Tier II
4 NC_000017.11 17 38568472 + SRCIN1 NNNCCTCGCAGAGTTACTGG NGG 2 0.6429 Tier II
5 NC_000015.10 15 53679746 - WDR72 NNNCTTCCCAAAGTAACTGG NGG 2 0.5789 Tier II
6 NC_000010.11 10 6423591 - PRKCQ NNNCTTCCCGGAGTTACTGA NGG 2 0.5208 Tier II
7 NC_000010.11 10 11196191 - CELF2 NNNCTTCCCAGGGTTACTGT NGG 2 0.5056 Tier II
8 NC_000016.10 16 17214302 - XYLT1 NNNCCTCCCAGAGTTAATGG NGG 2 0.4667 Tier II
9 NC_000010.11 10 31868599 + ARHGAP12 NNNCATTCCAGAGTTACTGG NGG 2 0.4063 Tier II
10 NC_000017.11 17 2376310 - SGSM2 NNNCTTCACAGAGTAACTGG NGG 2 0.3763 Tier II
11 NC_000008.11 8 69542574 + SULF1 NNNCTGCCGAGAGTTACTGG NGG 2 0.3537 Tier II
12 NC_000001.11 1 33772159 + CSMD2 NNNTTTCCCACAGTTACTGG NGG 2 0.3429 Tier II
13 NC_000002.12 2 164812596 - COBLL1 NNNCTTGCCAGAGTTACAGG NGG 2 0.3137 Tier II
14 NC_000007.14 7 3886471 - SDK1 NNNCTTCCAAGTGTTACTGG NGG 2 0.2857 Tier II
15 NC_000012.12 12 112175986 - HECTD4 NNNATTCCCAGAGTTACGGG NGG 2 0.2807 Tier II
16 NC_000009.12 9 132727045 - AK8 NNNCTTCCCATAGTTACAGG NGG 2 0.2564 Tier II
17 NC_000001.11 1 1475850 + ATAD3B NNNCTCCCCAGAGTCACTGG NGG 2 0.2479 Tier II
18 NC_000001.11 1 1514204 + ATAD3A NNNCTCCCCAGAGTCACTGG NGG 2 0.2479 Tier II
19 NC_000019.10 19 18716090 + CRTC1 NNNATTCCCAGAGTCACTGG NGG 2 0.2297 Tier II
20 NC_000004.12 4 78455066 - FRAS1 NNNCTTCTCAGAGTTACTGG NAG 2 0.2269 Tier II
21 NC_000002.12 2 166485710 - SCN7A NNNTTTCCCAGAGTTACTGG NAG 2 0.2074 Tier II
22 NC_000007.14 7 37006372 - ELMO1 NNNCTTCCCAGAGTCACTGT NGG 2 0.1909 Tier II
23 NC_000011.10 11 77938549 + INTS4 NNNCTTCCCAGAGTTACTGT NAG 2 0.1815 Tier II
24 NC_000011.10 11 35416433 + SLC1A2 NNNCTTCACAGAGTTACTGG NAG 2 0.1685 Tier II
25 NC_000001.11 1 66832037 + DNAI4 NNNCTTCCCAGAGATACTGG NAG 2 0.1605 Tier II
26 NC_000001.11 1 177018177 - ASTN1 NNNCTTCCCAGAGTTGCTTG NGG 2 0.1282 Tier II
27 NC_000006.12 6 142364599 - ADGRG6 NNNCTTCCCAGAGTTATTGG NAG 2 0.121 Tier II
28 NC_000003.12 3 2332430 - CNTN4 NNNCTTCTCAGAGTTACTGG NGA 2 0.0608 Tier II
29 NC_000008.11 8 120800327 - SNTB1 NNNCTTTCCAGAGTTACTGG NGA 2 0.0564 Tier II
30 NC_000002.12 2 179224641 - SESTD1 NNNCTTCTCAGAGTTAGTGG NGG 2 0.0515 Tier II
31 NC_000004.12 4 1217209 - CTBP1 NNNCTTCCCAGAGTGACTGG NGG 1 0.05 Tier II
32 NC_000010.11 10 48503579 - ARHGAP22 NNNCTTCCCAGAGTTGCTGG NAG 2 0.0499 Tier II
33 NC_000011.10 11 126341310 + DCPS NNNCTTCCCAGAGTGACTGA NGG 2 0.0469 Tier II
34 NC_000007.14 7 146505497 + CNTNAP2 NNNCTTGCCAGAGTTACTGG NGA 2 0.0327 Tier II
35 NC_000014.9 14 68483470 - RAD51B NNNCTTCCCAGAGTTACTTG NGT 2 0.0108 Tier II
36 NC_000007.14 7 155710805 + RBM33 NNNCTTACCAGAGTTCCTGG NGG 2 0.0 Tier II
37 NC_000001.11 1 46996714 - CYP4X1 NNNCTTCCAAGAGTTTCTGG NGG 2 0.0 Tier II
38 NC_000004.12 4 151307519 + SH3D19 NNNCTTCCCAGAGTTCCTGG NGC 2 0.0 Tier II
39 NC_000012.12 12 52619033 + KRT73 NNNCTTCCCAGAGTTCCTGG NGA 2 0.0 Tier II
40 NC_000010.11 10 11196191 - LOC124902375 NNNCTTCCCAGGGTTACTGT NGG 2 0.5056 Tier III
41 NC_000011.10 11 56874257 - LINC02735 NNNCTTCCCAGTGTTACTGG NGG 1 0.3333 Tier III
42 NC_000018.10 18 14679457 - GTF2IP8 NNNCTTCCCATAGTTACTGT NGG 2 0.2692 Tier III
43 NC_000005.10 5 55603259 - LOC124900980 NNNCTTCCCAGAGTTACTGA NAG 2 0.2431 Tier III
44 NC_000007.14 7 37006372 - ELMO1-AS1 NNNCTTCCCAGAGTCACTGT NGG 2 0.1909 Tier III
45 NC_000007.14 7 65170464 - INTS4P1 NNNCTTCCCAGAGTTACTGT NAG 2 0.1815 Tier III
46 NC_000007.14 7 65525527 + LOC101929322 NNNCTTCCCAGAGTTACTGT NAG 2 0.1815 Tier III
47 NC_000007.14 7 65677086 - LINC03006 NNNCTTCCCAGAGTTACTGT NAG 2 0.1815 Tier III
48 NC_000007.14 7 65677086 - INTS4P2 NNNCTTCCCAGAGTTACTGT NAG 2 0.1815 Tier III
49 NC_000004.12 4 1217209 - CTBP1-AS NNNCTTCCCAGAGTGACTGG NGG 1 0.05 Tier III
50 NC_000008.11 8 73329688 + RDH10-AS1 NNNCTTCCCAGAGTTAGTGC NGG 2 0.0252 Tier III
51 NC_000019.10 19 54320815 + VN1R104P NNNCTTCCCAGAGATACTGG NGT 2 0.01 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 60952140 + Camk2a NNNCTCCCCAGGGTTACTGG NGG 2 0.6566 Tier I
2 NC_000076.6 10 80135141 + Cbarp NNNCTCCCCAGAGTCACTGG NGG 2 0.2479 Tier I
3 NC_000070.6 4 24503079 + Mms22l NNNCTTCCCAGTGTTACTGT NGG 2 0.2333 Tier I
4 NC_000084.6 18 38899815 + Gm5820 NNNCTTCTCAGAGTTACTGG NAG 2 0.2269 Tier I
5 NC_000069.6 3 79705714 + Rxfp1 NNNCTTCCGAGAGTGACTGG NGG 2 0.031 Tier I
6 NC_000069.6 3 85661048 - Fam160a1 NNNCTTCCCAGAGTTCCTGA NGG 2 0.0 Tier I
7 NC_000076.6 10 92884652 - Cfap54 NNNCTTCCCAGAGTAACTGG NGG 1 0.5789 Tier II
8 NC_000067.6 1 136925930 - Nr5a2 NNNCTTCCAAGAGATACTGG NGG 2 0.5306 Tier II
9 NC_000074.6 8 107155779 - Cyb5b NNNTGTCCCAGAGTTACTGG NGG 2 0.512 Tier II
10 NC_000073.6 7 66853198 + Adamts17 NNNCTTCCCAGAGATACTGT NGG 2 0.4333 Tier II
11 NC_000067.6 1 22377547 - Rims1 NNNCATCCCAGAGTTACTTG NGG 2 0.3333 Tier II
12 NC_000084.6 18 38899815 + Fgf1 NNNCTTCTCAGAGTTACTGG NAG 2 0.2269 Tier II
13 NC_000084.6 18 38899815 + Arhgap26 NNNCTTCTCAGAGTTACTGG NAG 2 0.2269 Tier II
14 NC_000082.6 16 26048382 + P3h2 NNNCATCCCAGAGCTACTGG NGG 2 0.1429 Tier II
15 NC_000078.6 12 83580471 - Zfyve1 NNNCTTCCCAGGGTTGCTGG NGG 2 0.1389 Tier II
16 NC_000079.6 13 45564997 + Atxn1 NNNCTTCCCAGAGATACTGG NGA 2 0.043 Tier II
17 NC_000067.6 1 170402945 + Nos1ap NNNCTTCCCAGAGTTAGTGG NGT 2 0.0009 Tier II
18 NC_000083.6 17 72146044 + Alk NNNCTTCCCAGAGGTACTGG NGT 2 0.0 Tier II
19 NC_000068.7 2 49544527 + Epc2 NNNCTTCCCAGAGGTACTGT NGG 2 0.0 Tier II
20 NC_000072.6 6 53370754 + Creb5 NNNTTTCCCAGAGTTCCTGG NGG 2 0.0 Tier II
21 NC_000073.6 7 17318228 + Gm32772 NNNCTCCTCAGAGTTACTGG NGG 2 0.7955 Tier III
22 NC_000077.6 11 18850553 - 8430419K02Rik NNNCTGCCCAGAGTTCCTGG NGG 2 0.0 Tier III
23 NC_000072.6 6 66516727 + Tpm3-rs3 NNNCTTCCCAGAGTTCCAGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)