Construct: sgRNA BRDN0001146859
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCCTTCAACAGACTACTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GK5 (256356)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75773
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
142182928 |
- |
GK5 |
NNNCTTCAACAGACTACTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
86476095 |
- |
KDM3A |
NNNCTTAAACAGACTACAGG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000023.11 |
X |
155907198 |
- |
VAMP7 |
NNNCCTCAACAGACTACTGG |
NAG |
2 |
0.2593 |
Tier II |
| 4 |
NC_000024.10 |
Y |
57093718 |
- |
VAMP7 |
NNNCCTCAACAGACTACTGG |
NAG |
2 |
0.2593 |
Tier II |
| 5 |
NC_000014.9 |
14 |
76007979 |
+ |
IFT43 |
NNNCTTCAACAAACTACTGG |
NAG |
2 |
0.242 |
Tier II |
| 6 |
NC_000006.12 |
6 |
13434920 |
+ |
GFOD1 |
NNNCTTCAACAGACCACTTG |
NGG |
2 |
0.1818 |
Tier II |
| 7 |
NC_000001.11 |
1 |
15253825 |
- |
FHAD1 |
NNNCTGCAACAGACTACTGG |
NAG |
2 |
0.1481 |
Tier II |
| 8 |
NC_000006.12 |
6 |
56094489 |
- |
COL21A1 |
NNNCTTGAACAGACTACTGG |
NAG |
2 |
0.122 |
Tier II |
| 9 |
NC_000003.12 |
3 |
42841036 |
- |
ACKR2 |
NNNCTTCACCAGACTGCTGG |
NGG |
2 |
0.1099 |
Tier II |
| 10 |
NC_000011.10 |
11 |
82919125 |
- |
DDIAS |
NNNCTTCAACATACTACTGG |
NAG |
2 |
0.0997 |
Tier II |
| 11 |
NC_000017.11 |
17 |
68407902 |
- |
ARSG |
NNNCTTCAACATACTACTGG |
NAG |
2 |
0.0997 |
Tier II |
| 12 |
NC_000001.11 |
1 |
213194862 |
- |
RPS6KC1 |
NNNCTTCCACAGACTAGTGG |
NGG |
2 |
0.0252 |
Tier II |
| 13 |
NC_000002.12 |
2 |
240008074 |
+ |
NDUFA10 |
NNNCTTCAACAGACCACTGG |
NTG |
2 |
0.0106 |
Tier II |
| 14 |
NC_000005.10 |
5 |
35749337 |
+ |
SPEF2 |
NNNCTTCAACATAGTACTGG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000023.11 |
X |
75281294 |
- |
UPRT |
NNNCTTCAACATAGTACTGG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000009.12 |
9 |
106119704 |
+ |
LOC107987108 |
NNNCTTCAAAAGAATACTGG |
NGG |
2 |
0.3033 |
Tier III |
| 17 |
NC_000016.10 |
16 |
86056055 |
+ |
LOC105376778 |
NNNATTCAACAGTCTACTGG |
NGG |
2 |
0.2526 |
Tier III |
| 18 |
NC_000006.12 |
6 |
135596736 |
- |
AHI1-DT |
NNNCTTCAACACACTACTGG |
NAG |
2 |
0.1373 |
Tier III |
| 19 |
NC_000003.12 |
3 |
42841036 |
- |
LOC124909373 |
NNNCTTCACCAGACTGCTGG |
NGG |
2 |
0.1099 |
Tier III |
| 20 |
NC_000012.12 |
12 |
90932951 |
- |
LINC00615 |
NNNCTTCAACATACTACTGG |
NAG |
2 |
0.0997 |
Tier III |
| 21 |
NC_000009.12 |
9 |
89290256 |
- |
LOC105376136 |
NNNCTTCAACAGACTGCCGG |
NGG |
2 |
0.0824 |
Tier III |
| 22 |
NC_000004.12 |
4 |
10796791 |
+ |
LINC02498 |
NNNCTTCAACATAGTACTGG |
NGG |
2 |
0.0 |
Tier III |
| 23 |
NC_000008.11 |
8 |
110994504 |
+ |
LINC01608 |
NNNCTTCAACATAGTACTGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
74307252 |
+ |
Cfap53 |
NNNACTCAACAGACTACTGG |
NGG |
2 |
0.8421 |
Tier I |
| 2 |
NC_000075.6 |
9 |
116970253 |
- |
Rbms3 |
NNNCTTCAATAGATTACTGG |
NGG |
2 |
0.6902 |
Tier II |
| 3 |
NC_000071.6 |
5 |
53181699 |
+ |
Sel1l3 |
NNNCTACAACAGACTACTTG |
NGG |
2 |
0.5778 |
Tier II |
| 4 |
NC_000071.6 |
5 |
31831699 |
+ |
Babam2 |
NNNCTTCAAAAGACTACTTG |
NGG |
2 |
0.5778 |
Tier II |
| 5 |
NC_000076.6 |
10 |
11015631 |
+ |
Grm1 |
NNNCTAGAACAGACTACTGG |
NGG |
2 |
0.4078 |
Tier II |
| 6 |
NC_000067.6 |
1 |
172603360 |
- |
Fcrl6 |
NNNCTTCATCAGGCTACTGG |
NGG |
2 |
0.3913 |
Tier II |
| 7 |
NC_000086.7 |
X |
51432624 |
+ |
Hs6st2 |
NNNCTTCAATAGACTACTGG |
NAG |
2 |
0.244 |
Tier II |
| 8 |
NC_000069.6 |
3 |
89000921 |
- |
Ash1l |
NNNCTTCAACATACAACTGG |
NGG |
2 |
0.2227 |
Tier II |
| 9 |
NC_000081.6 |
15 |
58343393 |
+ |
Anxa13 |
NNNCATCAAGAGACTACTGG |
NGG |
2 |
0.1944 |
Tier II |
| 10 |
NC_000068.7 |
2 |
145909276 |
+ |
Naa20 |
NNNCTTCTACAGACTACTGC |
NGG |
2 |
0.1837 |
Tier II |
| 11 |
NC_000070.6 |
4 |
59895322 |
- |
Snx30 |
NNNCTTCAACAGATGACTGG |
NGG |
2 |
0.0367 |
Tier II |
| 12 |
NC_000069.6 |
3 |
147114716 |
- |
Gm29771 |
NNNCTTCCACAGACAACTGG |
NGG |
2 |
0.2481 |
Tier III |
| 13 |
NC_000086.7 |
X |
16233745 |
+ |
Cypt11-ps |
NNNCTTCAACAGACTACTGG |
NAC |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
