Construct: sgRNA BRDN0001146863
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCATCGTCAGACACATCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAST4 (375449)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76883
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
67163461 |
+ |
MAST4 |
NNNTCGTCAGACACATCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
216343578 |
+ |
MARCHF4 |
NNNGCGTCAGACACATTGTG |
NGG |
2 |
0.2917 |
Tier II |
| 3 |
NC_000002.12 |
2 |
86298033 |
+ |
REEP1 |
NNNTCGTCAGAGACATCCTG |
NGG |
2 |
0.2116 |
Tier II |
| 4 |
NC_000016.10 |
16 |
67479668 |
- |
ATP6V0D1 |
NNNTCGTCAGAAACTTCGTG |
NGG |
2 |
0.1429 |
Tier II |
| 5 |
NC_000013.11 |
13 |
39212782 |
+ |
LOC105370168 |
NNNTCTTCAGACAAATCGTG |
NGG |
2 |
0.2333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
102738584 |
- |
Mast4 |
NNNTCGTCAGACACATCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000084.6 |
18 |
69445530 |
+ |
Tcf4 |
NNNTCGTTGGACACATCGTG |
NGG |
2 |
0.5833 |
Tier II |
| 3 |
NC_000077.6 |
11 |
118087321 |
+ |
Dnah17 |
NNNTCGTCAGACACATGCTG |
NGG |
2 |
0.028 |
Tier II |
| 4 |
NC_000082.6 |
16 |
20195395 |
+ |
Yeats2 |
NNNTCGTCAGCCACATCGTG |
NGC |
2 |
0.0089 |
Tier II |
Other clones with same target sequence:
(none)
