Construct: sgRNA BRDN0001146864

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGCCACAAAGACCATCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD2 (914)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75827

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 116764539 - CD2 NNNCCACAAAGACCATCAAG NGG 0 1.0 Tier I
2 NC_000001.11 1 36137884 + TRAPPC3 NNNCCACAAAGTCCACCAAG NGG 2 0.2222 Tier I
3 NC_000010.11 10 101404654 - BTRC NNNCCACAATGGCCATCAAG NGG 2 0.6373 Tier II
4 NC_000017.11 17 19907741 - AKAP10 NNNAGACAAAGACCATCAAG NGG 2 0.5053 Tier II
5 NC_000011.10 11 131625295 - NTM NNNCCATAAAGACCATCATG NGG 2 0.4375 Tier II
6 NC_000020.11 20 8631688 - PLCB1 NNNCCACAATGACCATTAAG NGG 2 0.4118 Tier II
7 NC_000001.11 1 210791272 + KCNH1 NNNCCACAAAAAACATCAAG NGG 2 0.3846 Tier II
8 NC_000001.11 1 162157927 - NOS1AP NNNTCAGAAAGACCATCAAG NGG 2 0.3765 Tier II
9 NC_000013.11 13 31228043 - B3GLCT NNNCCACAAAGACCATCAGA NGG 2 0.3516 Tier II
10 NC_000020.11 20 41141148 - PLCG1 NNNCCACAAAGACCCTCAAA NGG 2 0.2557 Tier II
11 NC_000007.14 7 117210306 - ST7 NNNCCACAAAGGCCATCAAG NAG 2 0.1872 Tier II
12 NC_000017.11 17 9363162 + STX8 NNNCCTCAAAGACCATCCAG NGG 2 0.1361 Tier II
13 NC_000021.9 21 31249505 + TIAM1 NNNCCAGAAAGACCCTCAAG NGG 2 0.1283 Tier II
14 NC_000003.12 3 123307885 + ADCY5 NNNCCACAAGGACCATCCAG NGG 2 0.1058 Tier II
15 NC_000011.10 11 95120150 - ENDOD1 NNNCCACAGAGAGCATCAAG NGG 2 0.0909 Tier II
16 NC_000019.10 19 7147893 + INSR NNNCCAAAAAGACCATGAAG NGG 2 0.0441 Tier II
17 NC_000009.12 9 71091259 - TRPM3 NNNCCACAAAGACCATAAAG NGA 2 0.0324 Tier II
18 NC_000008.11 8 21984182 + XPO7 NNNCCACAAAGCCCATCAAG NGT 2 0.0042 Tier II
19 NC_000004.12 4 10536126 + CLNK NNNCCACAAAGACCATCCAG NGT 2 0.0031 Tier II
20 NC_000020.11 20 8631688 - LOC105372521 NNNCCACAATGACCATTAAG NGG 2 0.4118 Tier III
21 NC_000001.11 1 162157927 - LOC105371475 NNNTCAGAAAGACCATCAAG NGG 2 0.3765 Tier III
22 NC_000008.11 8 127330496 + CASC8 NNNCCACAAAGAACATCAAA NGG 2 0.3606 Tier III
23 NC_000008.11 8 127330496 + CASC21 NNNCCACAAAGAACATCAAA NGG 2 0.3606 Tier III
24 NC_000012.12 12 67351910 + MRPL40P1 NNNCCACTGAGACCATCAAG NGG 2 0.2857 Tier III
25 NC_000012.12 12 67351910 + LOC124902957 NNNCCACTGAGACCATCAAG NGG 2 0.2857 Tier III
26 NC_000008.11 8 88852505 - LOC105375630 NNNTCACAAAGACAATCAAG NGG 2 0.28 Tier III
27 NC_000005.10 5 89771894 + LINC02161 NNNCCTCAAAGACAATCAAG NGG 2 0.25 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 114984268 + Cd300ld NNNCCACAAAGACCATCAGC NGG 2 0.1607 Tier I
2 NC_000084.6 18 77010973 - Katnal2 NNNCCACAAAGACCAGCCAG NGG 2 0.0659 Tier I
3 NC_000086.7 X 73893231 - Avpr2 NNNCCACAAACACCATCAAG NCG 2 0.0459 Tier I
4 NC_000071.6 5 74212336 + Scfd2 NNNCCACAAAGAGGATCAAG NGG 2 0.0 Tier I
5 NC_000079.6 13 40190649 - Ofcc1 NNNTCACAATGACCATCAAG NGG 2 0.7059 Tier II
6 NC_000084.6 18 33952746 + Epb41l4a NNNTCACAGAGACCATCAAG NGG 2 0.5333 Tier II
7 NC_000076.6 10 109724854 + Nav3 NNNCCTCAAAGACCATCAAT NGG 2 0.5 Tier II
8 NC_000080.6 14 123742597 - Itgbl1 NNNCCACAGAGGCCATCAAG NGG 2 0.4815 Tier II
9 NC_000080.6 14 123850759 - Itgbl1 NNNACACAAAGACCATCAAC NGG 2 0.3609 Tier II
10 NC_000075.6 9 46091722 - Sik3 NNNCCACAAAGACCATCAGA NGG 2 0.3516 Tier II
11 NC_000068.7 2 129506313 - F830045P16Rik NNNCCAGGAAGACCATCAAG NGG 2 0.3451 Tier II
12 NC_000073.6 7 12846698 + Zfp110 NNNCAACATAGACCATCAAG NGG 2 0.3429 Tier II
13 NC_000075.6 9 76516973 - Fam83b NNNTCACAAAGACCATCAAC NGG 2 0.3429 Tier II
14 NC_000079.6 13 49440821 + Ippk NNNCCACAAAGGCCATAAAG NGG 2 0.337 Tier II
15 NC_000071.6 5 124021008 + Vps37b NNNCCACAGAGACCATAAAG NGG 2 0.3111 Tier II
16 NC_000070.6 4 123404096 + Macf1 NNNCCACAAAGGCCATCAAC NGG 2 0.3095 Tier II
17 NC_000070.6 4 123404096 + D830031N03Rik NNNCCACAAAGGCCATCAAC NGG 2 0.3095 Tier II
18 NC_000069.6 3 31218850 + Slc7a14 NNNACACAAAGACCAGCAAG NGG 2 0.2915 Tier II
19 NC_000071.6 5 90302261 - Ankrd17 NNNCCATAAAGACAATCAAG NGG 2 0.2844 Tier II
20 NC_000068.7 2 25266732 - Tprn NNNCCAGAAAGACCATCTAG NGG 2 0.2353 Tier II
21 NC_000075.6 9 45565328 + Dscaml1 NNNCCACACAGACCAGCAAG NGG 2 0.1978 Tier II
22 NC_000073.6 7 55979958 + Nipa1 NNNCCCCAAACACCATCAAG NGG 2 0.1948 Tier II
23 NC_000074.6 8 111085031 - Clec18a NNNCCAAAAAGACCATCAAG NAG 2 0.1944 Tier II
24 NC_000070.6 4 110404036 - Agbl4 NNNCCACAAAGACCATCCAG NGG 1 0.1905 Tier II
25 NC_000074.6 8 118486972 + Cdh13 NNNCCACTAACACCATCAAG NGG 2 0.1837 Tier II
26 NC_000079.6 13 109346560 - Pde4d NNNCCACAAAGACCAGAAAG NGG 2 0.1615 Tier II
27 NC_000080.6 14 22419755 - Lrmda NNNCCACAACCACCATCAAG NGG 2 0.1429 Tier II
28 NC_000070.6 4 98692691 + Patj NNNGCACAAAGCCCATCAAG NGG 2 0.1316 Tier II
29 NC_000080.6 14 27724286 + Erc2 NNNCCACAAAGACCATCTAG NAG 2 0.1296 Tier II
30 NC_000076.6 10 127461357 + R3hdm2 NNNTCACAAAGAGCATCAAG NGG 2 0.1091 Tier II
31 NC_000073.6 7 67146961 - Adamts17 NNNACACAAAGACCATCAAG NGA 2 0.0585 Tier II
32 NC_000067.6 1 159253967 - Cop1 NNNCCACAAAGACCACCAAG NGA 2 0.0463 Tier II
33 NC_000083.6 17 49686596 + Kif6 NNNCCACAAAGACCATCATG NTG 2 0.021 Tier II
34 NC_000067.6 1 104958534 + Cdh20 NNNCCACAAAGAACATCAAG NGT 2 0.0062 Tier II
35 NC_000068.7 2 130396021 + Cpxm1 NNNCCACAAAGACGAGCAAG NGG 2 0.0 Tier II
36 NC_000068.7 2 129506313 - Gm14040 NNNCCAGGAAGACCATCAAG NGG 2 0.3451 Tier III
37 NC_000080.6 14 71680749 + Gm34819 NNNCCTCCAAGACCATCAAG NGG 2 0.3061 Tier III
38 NC_000067.6 1 173714114 + Gm7871 NNNCCACAAAGGACATCAAG NGG 2 0.2778 Tier III
39 NC_000073.6 7 55979958 + A230056P14Rik NNNCCCCAAACACCATCAAG NGG 2 0.1948 Tier III
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Other clones with same target sequence:

(none)