Construct: sgRNA BRDN0001146865

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTTCAAAATAGTCAATGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NLK (51701)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75481

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 28132656 - NLK NNNTCAAAATAGTCAATGTG NGG 0 1.0 Tier I
2 NC_000019.10 19 34454221 + UBA2 NNNTCAAAATAGTCAATTTG NGG 1 0.6667 Tier II
3 NC_000018.10 18 76416647 + ZNF516 NNNTCTGAATAGTCAATGTG NGG 2 0.5042 Tier II
4 NC_000011.10 11 40784457 + LRRC4C NNNGCAAAATAGTCAATATG NGG 2 0.4327 Tier II
5 NC_000015.10 15 90639335 - CRTC3 NNNTAAAAATAGTCAATGTT NGG 2 0.4 Tier II
6 NC_000012.12 12 111327304 - CUX2 NNNTCAAAATAGTCAAAATG NGG 2 0.3692 Tier II
7 NC_000002.12 2 241367396 - FARP2 NNNACTAAATAGTCAATGTG NGG 2 0.3401 Tier II
8 NC_000020.11 20 59947673 - FAM217B NNNTTAAAATAGTCAATCTG NGG 2 0.303 Tier II
9 NC_000006.12 6 65520960 + EYS NNNTCAAAATAGTAAATTTG NGG 2 0.2333 Tier II
10 NC_000007.14 7 40738262 + SUGCT NNNTCAAACTAGTAAATGTG NGG 2 0.2 Tier II
11 NC_000023.11 X 48943532 - OTUD5 NNNTCAAAATAGTCAATACG NGG 2 0.191 Tier II
12 NC_000002.12 2 109007256 - RANBP2 NNNTCAAAATAGTAAAAGTG NGG 2 0.1867 Tier II
13 NC_000016.10 16 67328838 - LRRC36 NNNTCAAAATAGTCAGTGTA NGG 2 0.1803 Tier II
14 NC_000012.12 12 98959237 - ANKS1B NNNTCAAAATAATCAGTGTG NGG 2 0.1795 Tier II
15 NC_000011.10 11 132815107 - OPCML NNNTTAAAATAGTCCATGTG NGG 2 0.1736 Tier II
16 NC_000010.11 10 119826292 - INPP5F NNNTCAATATAGGCAATGTG NGG 2 0.1118 Tier II
17 NC_000015.10 15 83792921 + ADAMTSL3 NNNTCAAAATAGCCAAGGTG NGG 2 0.0929 Tier II
18 NC_000006.12 6 154164650 + OPRM1 NNNTCCAAATAGTCAGTGTG NGG 2 0.0874 Tier II
19 NC_000006.12 6 154164650 + IPCEF1 NNNTCCAAATAGTCAGTGTG NGG 2 0.0874 Tier II
20 NC_000002.12 2 48698547 - LHCGR NNNTCAAAATAGTCAATGAG NAG 2 0.0741 Tier II
21 NC_000002.12 2 48698547 - STON1-GTF2A1L NNNTCAAAATAGTCAATGAG NAG 2 0.0741 Tier II
22 NC_000005.10 5 83139206 - XRCC4 NNNTCAAAATAGTCAATGTC NTG 2 0.0167 Tier II
23 NC_000014.9 14 76027964 - IFT43 NNNTCAAAATAGTCAATGTC NTG 2 0.0167 Tier II
24 NC_000017.11 17 38942142 - FBXO47 NNNTCAAAATAATCACTGTG NGG 2 0.0 Tier II
25 NC_000016.10 16 75675459 + CPHXL2 NNNTCAAAATAATCATTGTG NGG 2 0.0 Tier II
26 NC_000020.11 20 15428380 - MACROD2 NNNTCAAAATAGTCAATGTG NCA 2 0.0 Tier II
27 NC_000005.10 5 176996407 + UIMC1 NNNTCAAAATAGTCACTGAG NGG 2 0.0 Tier II
28 NC_000013.11 13 35921020 - DCLK1 NNNTCAAAATAGTCATTGTG NAG 2 0.0 Tier II
29 NC_000018.10 18 24251799 + OSBPL1A NNNTCAAAATAGTCATTTTG NGG 2 0.0 Tier II
30 NC_000002.12 2 25765200 + ASXL2 NNNTGAAAATAGTCATTGTG NGG 2 0.0 Tier II
31 NC_000001.11 1 199116848 + LOC107985243 NNNTCAAAATAGCCAATTTG NGG 2 0.5263 Tier III
32 NC_000013.11 13 44404698 + TUSC8 NNNTCAAAGTAGACAATGTG NGG 2 0.4615 Tier III
33 NC_000015.10 15 90639335 - CRTC3-AS1 NNNTAAAAATAGTCAATGTT NGG 2 0.4 Tier III
34 NC_000019.10 19 56372835 + ZNF542P NNNTCAACAAAGTCAATGTG NGG 2 0.3673 Tier III
35 NC_000002.12 2 241367396 - LOC105373973 NNNACTAAATAGTCAATGTG NGG 2 0.3401 Tier III
36 NC_000003.12 3 192015221 + LOC105374277 NNNTAAACATAGTCAATGTG NGG 2 0.2449 Tier III
37 NC_000001.11 1 166282399 - LOC112268276 NNNTCACAACAGTCAATGTG NGG 2 0.2333 Tier III
38 NC_000005.10 5 113677802 - LOC107986441 NNNTCAACATAGTCAATGAG NGG 2 0.1224 Tier III
39 NC_000014.9 14 41087944 - LINC02315 NNNACAAAATAGTCAATGTG NGA 2 0.0331 Tier III
40 NC_000002.12 2 194910193 - LOC105376755 NNNTCAAAATATTCAATGTG NTG 2 0.015 Tier III
41 NC_000007.14 7 64654955 - LOC124901656 NNNTCAAAATGGTCAATGTG NGC 2 0.0144 Tier III
42 NC_000005.10 5 101759126 - LOC105379102 NNNTCAAAATAGTCAATGAG NTG 2 0.0111 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 78618295 + Nlk NNNTCAAAATAGTCAATGTG NGG 0 1.0 Tier I
2 NC_000075.6 9 38143781 - Olfr890 NNNTAAAAATAGTCAATGTG NGA 2 0.0397 Tier I
3 NC_000083.6 17 7071723 + Gm1604b NNNTCAAGAAAGTCAATGTG NGG 2 0.6286 Tier II
4 NC_000077.6 11 84999319 + Usp32 NNNTCAAAATACTCAATGTT NGG 2 0.3706 Tier II
5 NC_000068.7 2 157929041 - Vstm2l NNNTCAAAACAGTCAATATG NGG 2 0.3692 Tier II
6 NC_000068.7 2 113563980 - Fmn1 NNNGCAAAATACTCAATGTG NGG 2 0.3309 Tier II
7 NC_000083.6 17 49088157 - Lrfn2 NNNTCAAAATTGTCAAAGTG NGG 2 0.1641 Tier II
8 NC_000085.6 19 40168038 + Cyp2c70 NNNTCAAAATAGCCAGTGTG NGG 2 0.1518 Tier II
9 NC_000085.6 19 30051250 - Uhrf2 NNNTCAAAACAGTCAATGTG NAG 2 0.1383 Tier II
10 NC_000079.6 13 9559358 - Dip2c NNNTCAAAATAGTCAATGTC NAG 2 0.1111 Tier II
11 NC_000079.6 13 76886415 + Mctp1 NNNTCAAAATAGGAAATGTG NGG 2 0.0913 Tier II
12 NC_000068.7 2 105885604 - Elp4 NNNTCAAAATAGTCAAGGAG NGG 2 0.0336 Tier II
13 NC_000081.6 15 23147308 + Cdh18 NNNTCAAAATAGTCAAGGAG NGG 2 0.0336 Tier II
14 NC_000068.7 2 120478956 - Capn3 NNNTCAAAGTAGTCAATGTG NGC 2 0.0148 Tier II
15 NC_000073.6 7 49210846 + Nav2 NNNTCAAAATACTCACTGTG NGG 2 0.0 Tier II
16 NC_000083.6 17 84587465 + Plekhh2 NNNTCAAAATAGTCCCTGTG NGG 2 0.0 Tier II
17 NC_000083.6 17 8054430 - Gm1604a NNNTCAAGAAAGTCAATGTG NGG 2 0.6286 Tier III
18 NC_000068.7 2 164876081 - 4930445K14Rik NNNTCAGAATAGTCAATTTG NGG 2 0.4706 Tier III
19 NC_000075.6 9 25223420 + Gm29642 NNNTCAAAATAGTCCATGTG NGG 1 0.2727 Tier III
20 NC_000078.6 12 56603346 + Gm26973 NNNTCAAGATAGTAAATGTG NGG 2 0.2567 Tier III
21 NC_000068.7 2 87166727 + Nlk-ps1 NNNTCAAAATAGTCAGTGTG NGG 1 0.1923 Tier III
22 NC_000067.6 1 168679289 - Gm34320 NNNTCAAAATAATCAATGTG NGA 2 0.0648 Tier III
23 NC_000075.6 9 47177894 + Gm31698 NNNTCAAAATAATCAATGTG NGA 2 0.0648 Tier III
24 NC_000079.6 13 84741125 + Gm34196 NNNTCAAAATAGTCAATGTA NGT 2 0.0151 Tier III
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Other clones with same target sequence:

(none)