Construct: sgRNA BRDN0001146865
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTTCAAAATAGTCAATGTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- NLK (51701)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000017.11 | 17 | 28132656 | - | NLK | NNNTCAAAATAGTCAATGTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000019.10 | 19 | 34454221 | + | UBA2 | NNNTCAAAATAGTCAATTTG | NGG | 1 | 0.6667 | Tier II |
3 | NC_000018.10 | 18 | 76416647 | + | ZNF516 | NNNTCTGAATAGTCAATGTG | NGG | 2 | 0.5042 | Tier II |
4 | NC_000011.10 | 11 | 40784457 | + | LRRC4C | NNNGCAAAATAGTCAATATG | NGG | 2 | 0.4327 | Tier II |
5 | NC_000015.10 | 15 | 90639335 | - | CRTC3 | NNNTAAAAATAGTCAATGTT | NGG | 2 | 0.4 | Tier II |
6 | NC_000012.12 | 12 | 111327304 | - | CUX2 | NNNTCAAAATAGTCAAAATG | NGG | 2 | 0.3692 | Tier II |
7 | NC_000002.12 | 2 | 241367396 | - | FARP2 | NNNACTAAATAGTCAATGTG | NGG | 2 | 0.3401 | Tier II |
8 | NC_000020.11 | 20 | 59947673 | - | FAM217B | NNNTTAAAATAGTCAATCTG | NGG | 2 | 0.303 | Tier II |
9 | NC_000006.12 | 6 | 65520960 | + | EYS | NNNTCAAAATAGTAAATTTG | NGG | 2 | 0.2333 | Tier II |
10 | NC_000007.14 | 7 | 40738262 | + | SUGCT | NNNTCAAACTAGTAAATGTG | NGG | 2 | 0.2 | Tier II |
11 | NC_000023.11 | X | 48943532 | - | OTUD5 | NNNTCAAAATAGTCAATACG | NGG | 2 | 0.191 | Tier II |
12 | NC_000002.12 | 2 | 109007256 | - | RANBP2 | NNNTCAAAATAGTAAAAGTG | NGG | 2 | 0.1867 | Tier II |
13 | NC_000016.10 | 16 | 67328838 | - | LRRC36 | NNNTCAAAATAGTCAGTGTA | NGG | 2 | 0.1803 | Tier II |
14 | NC_000012.12 | 12 | 98959237 | - | ANKS1B | NNNTCAAAATAATCAGTGTG | NGG | 2 | 0.1795 | Tier II |
15 | NC_000011.10 | 11 | 132815107 | - | OPCML | NNNTTAAAATAGTCCATGTG | NGG | 2 | 0.1736 | Tier II |
16 | NC_000010.11 | 10 | 119826292 | - | INPP5F | NNNTCAATATAGGCAATGTG | NGG | 2 | 0.1118 | Tier II |
17 | NC_000015.10 | 15 | 83792921 | + | ADAMTSL3 | NNNTCAAAATAGCCAAGGTG | NGG | 2 | 0.0929 | Tier II |
18 | NC_000006.12 | 6 | 154164650 | + | OPRM1 | NNNTCCAAATAGTCAGTGTG | NGG | 2 | 0.0874 | Tier II |
19 | NC_000006.12 | 6 | 154164650 | + | IPCEF1 | NNNTCCAAATAGTCAGTGTG | NGG | 2 | 0.0874 | Tier II |
20 | NC_000002.12 | 2 | 48698547 | - | LHCGR | NNNTCAAAATAGTCAATGAG | NAG | 2 | 0.0741 | Tier II |
21 | NC_000002.12 | 2 | 48698547 | - | STON1-GTF2A1L | NNNTCAAAATAGTCAATGAG | NAG | 2 | 0.0741 | Tier II |
22 | NC_000005.10 | 5 | 83139206 | - | XRCC4 | NNNTCAAAATAGTCAATGTC | NTG | 2 | 0.0167 | Tier II |
23 | NC_000014.9 | 14 | 76027964 | - | IFT43 | NNNTCAAAATAGTCAATGTC | NTG | 2 | 0.0167 | Tier II |
24 | NC_000017.11 | 17 | 38942142 | - | FBXO47 | NNNTCAAAATAATCACTGTG | NGG | 2 | 0.0 | Tier II |
25 | NC_000016.10 | 16 | 75675459 | + | CPHXL2 | NNNTCAAAATAATCATTGTG | NGG | 2 | 0.0 | Tier II |
26 | NC_000020.11 | 20 | 15428380 | - | MACROD2 | NNNTCAAAATAGTCAATGTG | NCA | 2 | 0.0 | Tier II |
27 | NC_000005.10 | 5 | 176996407 | + | UIMC1 | NNNTCAAAATAGTCACTGAG | NGG | 2 | 0.0 | Tier II |
28 | NC_000013.11 | 13 | 35921020 | - | DCLK1 | NNNTCAAAATAGTCATTGTG | NAG | 2 | 0.0 | Tier II |
29 | NC_000018.10 | 18 | 24251799 | + | OSBPL1A | NNNTCAAAATAGTCATTTTG | NGG | 2 | 0.0 | Tier II |
30 | NC_000002.12 | 2 | 25765200 | + | ASXL2 | NNNTGAAAATAGTCATTGTG | NGG | 2 | 0.0 | Tier II |
31 | NC_000001.11 | 1 | 199116848 | + | LOC107985243 | NNNTCAAAATAGCCAATTTG | NGG | 2 | 0.5263 | Tier III |
32 | NC_000013.11 | 13 | 44404698 | + | TUSC8 | NNNTCAAAGTAGACAATGTG | NGG | 2 | 0.4615 | Tier III |
33 | NC_000015.10 | 15 | 90639335 | - | CRTC3-AS1 | NNNTAAAAATAGTCAATGTT | NGG | 2 | 0.4 | Tier III |
34 | NC_000019.10 | 19 | 56372835 | + | ZNF542P | NNNTCAACAAAGTCAATGTG | NGG | 2 | 0.3673 | Tier III |
35 | NC_000002.12 | 2 | 241367396 | - | LOC105373973 | NNNACTAAATAGTCAATGTG | NGG | 2 | 0.3401 | Tier III |
36 | NC_000003.12 | 3 | 192015221 | + | LOC105374277 | NNNTAAACATAGTCAATGTG | NGG | 2 | 0.2449 | Tier III |
37 | NC_000001.11 | 1 | 166282399 | - | LOC112268276 | NNNTCACAACAGTCAATGTG | NGG | 2 | 0.2333 | Tier III |
38 | NC_000005.10 | 5 | 113677802 | - | LOC107986441 | NNNTCAACATAGTCAATGAG | NGG | 2 | 0.1224 | Tier III |
39 | NC_000014.9 | 14 | 41087944 | - | LINC02315 | NNNACAAAATAGTCAATGTG | NGA | 2 | 0.0331 | Tier III |
40 | NC_000002.12 | 2 | 194910193 | - | LOC105376755 | NNNTCAAAATATTCAATGTG | NTG | 2 | 0.015 | Tier III |
41 | NC_000007.14 | 7 | 64654955 | - | LOC124901656 | NNNTCAAAATGGTCAATGTG | NGC | 2 | 0.0144 | Tier III |
42 | NC_000005.10 | 5 | 101759126 | - | LOC105379102 | NNNTCAAAATAGTCAATGAG | NTG | 2 | 0.0111 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000077.6 | 11 | 78618295 | + | Nlk | NNNTCAAAATAGTCAATGTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000075.6 | 9 | 38143781 | - | Olfr890 | NNNTAAAAATAGTCAATGTG | NGA | 2 | 0.0397 | Tier I |
3 | NC_000083.6 | 17 | 7071723 | + | Gm1604b | NNNTCAAGAAAGTCAATGTG | NGG | 2 | 0.6286 | Tier II |
4 | NC_000077.6 | 11 | 84999319 | + | Usp32 | NNNTCAAAATACTCAATGTT | NGG | 2 | 0.3706 | Tier II |
5 | NC_000068.7 | 2 | 157929041 | - | Vstm2l | NNNTCAAAACAGTCAATATG | NGG | 2 | 0.3692 | Tier II |
6 | NC_000068.7 | 2 | 113563980 | - | Fmn1 | NNNGCAAAATACTCAATGTG | NGG | 2 | 0.3309 | Tier II |
7 | NC_000083.6 | 17 | 49088157 | - | Lrfn2 | NNNTCAAAATTGTCAAAGTG | NGG | 2 | 0.1641 | Tier II |
8 | NC_000085.6 | 19 | 40168038 | + | Cyp2c70 | NNNTCAAAATAGCCAGTGTG | NGG | 2 | 0.1518 | Tier II |
9 | NC_000085.6 | 19 | 30051250 | - | Uhrf2 | NNNTCAAAACAGTCAATGTG | NAG | 2 | 0.1383 | Tier II |
10 | NC_000079.6 | 13 | 9559358 | - | Dip2c | NNNTCAAAATAGTCAATGTC | NAG | 2 | 0.1111 | Tier II |
11 | NC_000079.6 | 13 | 76886415 | + | Mctp1 | NNNTCAAAATAGGAAATGTG | NGG | 2 | 0.0913 | Tier II |
12 | NC_000068.7 | 2 | 105885604 | - | Elp4 | NNNTCAAAATAGTCAAGGAG | NGG | 2 | 0.0336 | Tier II |
13 | NC_000081.6 | 15 | 23147308 | + | Cdh18 | NNNTCAAAATAGTCAAGGAG | NGG | 2 | 0.0336 | Tier II |
14 | NC_000068.7 | 2 | 120478956 | - | Capn3 | NNNTCAAAGTAGTCAATGTG | NGC | 2 | 0.0148 | Tier II |
15 | NC_000073.6 | 7 | 49210846 | + | Nav2 | NNNTCAAAATACTCACTGTG | NGG | 2 | 0.0 | Tier II |
16 | NC_000083.6 | 17 | 84587465 | + | Plekhh2 | NNNTCAAAATAGTCCCTGTG | NGG | 2 | 0.0 | Tier II |
17 | NC_000083.6 | 17 | 8054430 | - | Gm1604a | NNNTCAAGAAAGTCAATGTG | NGG | 2 | 0.6286 | Tier III |
18 | NC_000068.7 | 2 | 164876081 | - | 4930445K14Rik | NNNTCAGAATAGTCAATTTG | NGG | 2 | 0.4706 | Tier III |
19 | NC_000075.6 | 9 | 25223420 | + | Gm29642 | NNNTCAAAATAGTCCATGTG | NGG | 1 | 0.2727 | Tier III |
20 | NC_000078.6 | 12 | 56603346 | + | Gm26973 | NNNTCAAGATAGTAAATGTG | NGG | 2 | 0.2567 | Tier III |
21 | NC_000068.7 | 2 | 87166727 | + | Nlk-ps1 | NNNTCAAAATAGTCAGTGTG | NGG | 1 | 0.1923 | Tier III |
22 | NC_000067.6 | 1 | 168679289 | - | Gm34320 | NNNTCAAAATAATCAATGTG | NGA | 2 | 0.0648 | Tier III |
23 | NC_000075.6 | 9 | 47177894 | + | Gm31698 | NNNTCAAAATAATCAATGTG | NGA | 2 | 0.0648 | Tier III |
24 | NC_000079.6 | 13 | 84741125 | + | Gm34196 | NNNTCAAAATAGTCAATGTA | NGT | 2 | 0.0151 | Tier III |