Construct: sgRNA BRDN0001146868
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTTCATGTAGATACTCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AKT3 (10000)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76217
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000001.11 |
1 |
243664782 |
- |
AKT3 |
NNNTCATGTAGATACTCCAG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000005.10 |
5 |
111446683 |
- |
CAMK4 |
NNNTCATGTAGATACTAAAG |
NGG |
2 |
0.2513 |
Tier I |
3 |
NC_000010.11 |
10 |
100488060 |
+ |
SEC31B |
NNNTCATATAGATACTCCAG |
NCG |
2 |
0.1071 |
Tier I |
4 |
NC_000016.10 |
16 |
53848366 |
+ |
FTO |
NNNTCAAGTAAATACTCCAG |
NGG |
2 |
0.875 |
Tier II |
5 |
NC_000001.11 |
1 |
204948398 |
+ |
NFASC |
NNNTCAAGTAGATTCTCCAG |
NGG |
2 |
0.4667 |
Tier II |
6 |
NC_000002.12 |
2 |
66507023 |
+ |
MEIS1 |
NNNTCATGTAGATACTCTAT |
NGG |
2 |
0.45 |
Tier II |
7 |
NC_000003.12 |
3 |
123717597 |
+ |
MYLK |
NNNTGATGGAGATACTCCAG |
NGG |
2 |
0.3714 |
Tier II |
8 |
NC_000010.11 |
10 |
132078679 |
+ |
JAKMIP3 |
NNNTCATGTAGCTACTCCTG |
NGG |
2 |
0.1417 |
Tier II |
9 |
NC_000008.11 |
8 |
40829302 |
- |
ZMAT4 |
NNNTCATTTAGATCCTCCAG |
NGG |
2 |
0.1339 |
Tier II |
10 |
NC_000012.12 |
12 |
79601341 |
- |
PAWR |
NNNACATGTAGCTACTCCAG |
NGG |
2 |
0.1253 |
Tier II |
11 |
NC_000004.12 |
4 |
78885225 |
+ |
BMP2K |
NNNTCATGTAGATACTGCAG |
NGT |
2 |
0.0009 |
Tier II |
12 |
NC_000010.11 |
10 |
89419702 |
+ |
IFIT5 |
NNNTCATGTAGATACTCCAG |
NAA |
2 |
0.0 |
Tier II |
13 |
NC_000005.10 |
5 |
4487691 |
- |
LOC124901169 |
NNNTCATCTAGTTACTCCAG |
NGG |
2 |
0.2051 |
Tier III |
14 |
NC_000007.14 |
7 |
142397801 |
+ |
TRB |
NNNTCATGCAGATACTCCAG |
NGA |
2 |
0.0641 |
Tier III |
15 |
NC_000019.10 |
19 |
3297976 |
+ |
LOC105372244 |
NNNTCATGGAGATAGTCCAG |
NGG |
2 |
0.031 |
Tier III |
16 |
NC_000010.11 |
10 |
89419702 |
+ |
LOC107984251 |
NNNTCATGTAGATACTCCAG |
NAA |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000067.6 |
1 |
177109637 |
- |
Akt3 |
NNNTCATGTAGATACACCAG |
NGG |
1 |
0.9091 |
Tier I |
2 |
NC_000076.6 |
10 |
9808553 |
- |
Stxbp5 |
NNNTGATGTGGATACTCCAG |
NGG |
2 |
0.3333 |
Tier I |
3 |
NC_000070.6 |
4 |
6415067 |
- |
Nsmaf |
NNNTCATGTAGTTTCTCCAG |
NGG |
2 |
0.1778 |
Tier I |
4 |
NC_000073.6 |
7 |
44767134 |
- |
Vrk3 |
NNNTCATGGAGATACTCCAG |
NGC |
2 |
0.0138 |
Tier I |
5 |
NC_000080.6 |
14 |
59740784 |
- |
Atp8a2 |
NNNTCATGTAGATACATCAG |
NGG |
2 |
0.4242 |
Tier II |
6 |
NC_000075.6 |
9 |
20712584 |
+ |
Olfm2 |
NNNTCATATAGTTACTCCAG |
NGG |
2 |
0.3333 |
Tier II |
7 |
NC_000067.6 |
1 |
126611544 |
- |
Nckap5 |
NNNTCATGTGGATACTCCTG |
NGG |
2 |
0.2991 |
Tier II |
8 |
NC_000080.6 |
14 |
30219122 |
- |
Cacna1d |
NNNTCATGGAGATACTACAG |
NGG |
2 |
0.2889 |
Tier II |
9 |
NC_000082.6 |
16 |
48319207 |
+ |
Dppa2 |
NNNTCATGTAGCAACTCCAG |
NGG |
2 |
0.1822 |
Tier II |
10 |
NC_000067.6 |
1 |
181993029 |
+ |
Enah |
NNNTCATCTAGATACTCCAG |
NAG |
2 |
0.1595 |
Tier II |
11 |
NC_000067.6 |
1 |
129853161 |
- |
Thsd7b |
NNNTCATGTAGACACTGCAG |
NGG |
2 |
0.0464 |
Tier II |
12 |
NC_000086.7 |
X |
42533348 |
- |
Tenm1 |
NNNTGATGTAGATATTCCAG |
NGG |
2 |
0.04 |
Tier II |
13 |
NC_000080.6 |
14 |
65841453 |
+ |
Ccdc25 |
NNNTCATGTAGCTACTGCAG |
NGG |
2 |
0.0155 |
Tier II |
14 |
NC_000086.7 |
X |
137078142 |
+ |
Fam199x |
NNNTCATGTAGATAATCCAG |
NGA |
2 |
0.0154 |
Tier II |
15 |
NC_000079.6 |
13 |
95630395 |
- |
Iqgap2 |
NNNTCATGTAGTTACTCCAG |
NGC |
2 |
0.0074 |
Tier II |
16 |
NC_000069.6 |
3 |
18635542 |
- |
Gm30789 |
NNNTAGTGTAGATACTCCAG |
NGG |
2 |
0.4082 |
Tier III |
17 |
NC_000069.6 |
3 |
36399025 |
+ |
Gm42209 |
NNNACATGTAGACACTCCAG |
NGG |
2 |
0.3759 |
Tier III |
18 |
NC_000070.6 |
4 |
9788216 |
- |
4930412C18Rik |
NNNTTCTGTAGATACTCCAG |
NGG |
2 |
0.2893 |
Tier III |
19 |
NC_000080.6 |
14 |
30219122 |
- |
Gm52107 |
NNNTCATGGAGATACTACAG |
NGG |
2 |
0.2889 |
Tier III |
20 |
NC_000085.6 |
19 |
41461145 |
+ |
Gm9788 |
NNNTCATGTAGCAACTCCAG |
NGG |
2 |
0.1822 |
Tier III |
21 |
NC_000069.6 |
3 |
54510380 |
- |
Gm31154 |
NNNTTATGTAGATAATCCAG |
NGG |
2 |
0.1414 |
Tier III |
Other clones with same target sequence:
(none)