Construct: sgRNA BRDN0001146868

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTTCATGTAGATACTCCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKT3 (10000)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76217

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 243664782 - AKT3 NNNTCATGTAGATACTCCAG NGG 0 1.0 Tier I
2 NC_000005.10 5 111446683 - CAMK4 NNNTCATGTAGATACTAAAG NGG 2 0.2513 Tier I
3 NC_000010.11 10 100488060 + SEC31B NNNTCATATAGATACTCCAG NCG 2 0.1071 Tier I
4 NC_000016.10 16 53848366 + FTO NNNTCAAGTAAATACTCCAG NGG 2 0.875 Tier II
5 NC_000001.11 1 204948398 + NFASC NNNTCAAGTAGATTCTCCAG NGG 2 0.4667 Tier II
6 NC_000002.12 2 66507023 + MEIS1 NNNTCATGTAGATACTCTAT NGG 2 0.45 Tier II
7 NC_000003.12 3 123717597 + MYLK NNNTGATGGAGATACTCCAG NGG 2 0.3714 Tier II
8 NC_000010.11 10 132078679 + JAKMIP3 NNNTCATGTAGCTACTCCTG NGG 2 0.1417 Tier II
9 NC_000008.11 8 40829302 - ZMAT4 NNNTCATTTAGATCCTCCAG NGG 2 0.1339 Tier II
10 NC_000012.12 12 79601341 - PAWR NNNACATGTAGCTACTCCAG NGG 2 0.1253 Tier II
11 NC_000004.12 4 78885225 + BMP2K NNNTCATGTAGATACTGCAG NGT 2 0.0009 Tier II
12 NC_000010.11 10 89419702 + IFIT5 NNNTCATGTAGATACTCCAG NAA 2 0.0 Tier II
13 NC_000005.10 5 4487691 - LOC124901169 NNNTCATCTAGTTACTCCAG NGG 2 0.2051 Tier III
14 NC_000007.14 7 142397801 + TRB NNNTCATGCAGATACTCCAG NGA 2 0.0641 Tier III
15 NC_000019.10 19 3297976 + LOC105372244 NNNTCATGGAGATAGTCCAG NGG 2 0.031 Tier III
16 NC_000010.11 10 89419702 + LOC107984251 NNNTCATGTAGATACTCCAG NAA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 177109637 - Akt3 NNNTCATGTAGATACACCAG NGG 1 0.9091 Tier I
2 NC_000076.6 10 9808553 - Stxbp5 NNNTGATGTGGATACTCCAG NGG 2 0.3333 Tier I
3 NC_000070.6 4 6415067 - Nsmaf NNNTCATGTAGTTTCTCCAG NGG 2 0.1778 Tier I
4 NC_000073.6 7 44767134 - Vrk3 NNNTCATGGAGATACTCCAG NGC 2 0.0138 Tier I
5 NC_000080.6 14 59740784 - Atp8a2 NNNTCATGTAGATACATCAG NGG 2 0.4242 Tier II
6 NC_000075.6 9 20712584 + Olfm2 NNNTCATATAGTTACTCCAG NGG 2 0.3333 Tier II
7 NC_000067.6 1 126611544 - Nckap5 NNNTCATGTGGATACTCCTG NGG 2 0.2991 Tier II
8 NC_000080.6 14 30219122 - Cacna1d NNNTCATGGAGATACTACAG NGG 2 0.2889 Tier II
9 NC_000082.6 16 48319207 + Dppa2 NNNTCATGTAGCAACTCCAG NGG 2 0.1822 Tier II
10 NC_000067.6 1 181993029 + Enah NNNTCATCTAGATACTCCAG NAG 2 0.1595 Tier II
11 NC_000067.6 1 129853161 - Thsd7b NNNTCATGTAGACACTGCAG NGG 2 0.0464 Tier II
12 NC_000086.7 X 42533348 - Tenm1 NNNTGATGTAGATATTCCAG NGG 2 0.04 Tier II
13 NC_000080.6 14 65841453 + Ccdc25 NNNTCATGTAGCTACTGCAG NGG 2 0.0155 Tier II
14 NC_000086.7 X 137078142 + Fam199x NNNTCATGTAGATAATCCAG NGA 2 0.0154 Tier II
15 NC_000079.6 13 95630395 - Iqgap2 NNNTCATGTAGTTACTCCAG NGC 2 0.0074 Tier II
16 NC_000069.6 3 18635542 - Gm30789 NNNTAGTGTAGATACTCCAG NGG 2 0.4082 Tier III
17 NC_000069.6 3 36399025 + Gm42209 NNNACATGTAGACACTCCAG NGG 2 0.3759 Tier III
18 NC_000070.6 4 9788216 - 4930412C18Rik NNNTTCTGTAGATACTCCAG NGG 2 0.2893 Tier III
19 NC_000080.6 14 30219122 - Gm52107 NNNTCATGGAGATACTACAG NGG 2 0.2889 Tier III
20 NC_000085.6 19 41461145 + Gm9788 NNNTCATGTAGCAACTCCAG NGG 2 0.1822 Tier III
21 NC_000069.6 3 54510380 - Gm31154 NNNTTATGTAGATAATCCAG NGG 2 0.1414 Tier III
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Other clones with same target sequence:

(none)