Construct: sgRNA BRDN0001146869

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGATGCCTACCGGGTACCGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KSR1 (8844)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77858

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 27603882 - KSR1 NNNTGCCTACCGGGTACCGT NGG 0 1.0 Tier I
2 NC_000022.11 22 28003948 + TTC28 NNNTGCCTACTGGGGACCGT NGG 2 0.0154 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 145234462 - Tnfrsf1b NNNTGCCTACCAGGTACAGT NGG 2 0.5026 Tier II
2 NC_000072.6 6 120110913 - Ninj2 NNNTGCCTACATGGTACCGT NGG 2 0.2885 Tier II
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Other clones with same target sequence:

(none)