Construct: sgRNA BRDN0001146874

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGACAATGCCATAGTTGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K20 (51776)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75598

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 173169874 - MAP3K20 NNNACAATGCCATAGTTGGG NGG 0 1.0 Tier I
2 NC_000020.11 20 36793513 + MTCL2 NNNACAATGCCGTACTTGGG NGG 2 0.197 Tier I
3 NC_000017.11 17 1145583 - ABR NNNACAAAGCCATAGATGGG NGG 2 0.7273 Tier II
4 NC_000023.11 X 73586938 - CHIC1 NNNACAATGCCACAGTTGAG NGG 2 0.5639 Tier II
5 NC_000003.12 3 183312245 - MCF2L2 NNNACAAGGCCATAGTTGGT NGG 2 0.5133 Tier II
6 NC_000022.11 22 25622876 + GRK3 NNNTCAATGCCATAGTTTGG NGG 2 0.4242 Tier II
7 NC_000008.11 8 1131304 + DLGAP2 NNNACATTGCCATAGATGGG NGG 2 0.3977 Tier II
8 NC_000017.11 17 61102546 - BCAS3 NNNACACTGCCAAAGTTGGG NGG 2 0.3029 Tier II
9 NC_000001.11 1 5863744 - NPHP4 NNNACCATCCCATAGTTGGG NGG 2 0.2448 Tier II
10 NC_000005.10 5 133397041 - FSTL4 NNNACAATGTCATAGTTGGG NAG 2 0.244 Tier II
11 NC_000008.11 8 118326622 - SAMD12 NNNACAATTCCTTAGTTGGG NGG 2 0.1778 Tier II
12 NC_000013.11 13 41614419 + VWA8 NNNACAATGCCCTAGTTGGG NAG 2 0.0682 Tier II
13 NC_000007.14 7 34756536 - NPSR1 NNNACAATGCCAGAGTTGGG NAG 2 0.0676 Tier II
14 NC_000001.11 1 187318947 + LINC01036 NNNAGAATACCATAGTTGGG NGG 2 0.3857 Tier III
15 NC_000017.11 17 61102546 - BCAS3-AS1 NNNACACTGCCAAAGTTGGG NGG 2 0.3029 Tier III
16 NC_000017.11 17 61102546 - LOC124904041 NNNACACTGCCAAAGTTGGG NGG 2 0.3029 Tier III
17 NC_000008.11 8 118326622 - LOC105375724 NNNACAATTCCTTAGTTGGG NGG 2 0.1778 Tier III
18 NC_000018.10 18 14997661 + LOC100887076 NNNACACTGCGATAGTTGGG NGG 2 0.1094 Tier III
19 NC_000003.12 3 59438150 + CFAP20DC-DT NNNACAATGGCAGAGTTGGG NGG 2 0.1014 Tier III
20 NC_000017.11 17 36006142 - LOC107985068 NNNACAATGCCCTACTTGGG NGG 2 0.0718 Tier III
21 NC_000007.14 7 34756536 - NPSR1-AS1 NNNACAATGCCAGAGTTGGG NAG 2 0.0676 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 72355627 - Map3k20 NNNACGATGCCATAGTTGGG NGG 1 0.7143 Tier I
2 NC_000068.7 2 157020454 + Soga1 NNNACAATGCCATACTTGGG NGG 1 0.2727 Tier I
3 NC_000074.6 8 70786195 + Mast3 NNNACAATGCCATAGTTGTG NAG 2 0.1728 Tier I
4 NC_000071.6 5 8623395 + Abcb1a NNNAGAATGCCATAGTTGGG NGG 1 0.6 Tier II
5 NC_000071.6 5 150627686 - N4bp2l2 NNNAAAATGTCATAGTTGGG NGG 2 0.5378 Tier II
6 NC_000068.7 2 181406396 + Zbtb46 NNNACAATGCCAAAGTTGGT NGG 2 0.4846 Tier II
7 NC_000070.6 4 124992068 + Rspo1 NNNACAATGCCAAAGTTGGT NGG 2 0.4846 Tier II
8 NC_000070.6 4 153971176 - Dffb NNNACATTGCCATAATTGGG NGG 2 0.4118 Tier II
9 NC_000085.6 19 44782901 + Pax2 NNNACAATTCCAAAGTTGGG NGG 2 0.3692 Tier II
10 NC_000083.6 17 5319222 + Arid1b NNNATAATTCCATAGTTGGG NGG 2 0.3394 Tier II
11 NC_000080.6 14 55763149 - Ltb4r2 NNNACAATGCCATGGTTTGG NGG 2 0.3111 Tier II
12 NC_000085.6 19 9130356 + Asrgl1 NNNACAAAGCCAGAGTTGGG NGG 2 0.2087 Tier II
13 NC_000073.6 7 123109750 + Tnrc6a NNNACAATGCCAGTGTTGGG NGG 2 0.1391 Tier II
14 NC_000073.6 7 96345095 + Tenm4 NNNACAATGGCATCGTTGGG NGG 2 0.0833 Tier II
15 NC_000075.6 9 117315859 + Rbms3 NNNACAATGCCATCGTTGGG NAG 2 0.0556 Tier II
16 NC_000074.6 8 23846932 + Zmat4 NNNACAATGCCATCTTTGGG NGG 2 0.0306 Tier II
17 NC_000083.6 17 66997362 - Ptprm NNNACAAAGCCATAGTTGGG NGC 2 0.0178 Tier II
18 NC_000075.6 9 80991650 - Gm39381 NNNACAATGTCATAGTTGGC NGG 2 0.4034 Tier III
19 NC_000075.6 9 117315859 + Gm39451 NNNACAATGCCATCGTTGGG NAG 2 0.0556 Tier III
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Other clones with same target sequence:

(none)