Construct: sgRNA BRDN0001146877

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAGTGTGTACCTTCCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGFR1 (2260)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76068

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 38421803 + FGFR1 NNNGTGTGTACCTTCCAGAA NGG 0 1.0 Tier I
2 NC_000014.9 14 80505005 + CEP128 NNNGTGGGAACCTTCCAGAA NGG 2 0.5462 Tier I
3 NC_000023.11 X 47225097 - CDK16 NNNGTGTGTACCTTCCCGGA NGG 2 0.0662 Tier I
4 NC_000014.9 14 67432481 + GPHN NNNGTATTTACCTTCCAGAA NGG 2 0.625 Tier II
5 NC_000006.12 6 151451532 + RMND1 NNNGTGTCTTCCTTCCAGAA NGG 2 0.543 Tier II
6 NC_000014.9 14 102986252 - CDC42BPB NNNATGTGTGCCTTCCAGAA NGG 2 0.5 Tier II
7 NC_000005.10 5 22840687 + CDH12 NNNGTGTTTACATTCCAGAA NGG 2 0.4464 Tier II
8 NC_000011.10 11 108570054 + EXPH5 NNNCTGTCTACCTTCCAGAA NGG 2 0.3258 Tier II
9 NC_000001.11 1 89121563 + GBP2 NNNTTGTGTTCCTTCCAGAA NGG 2 0.3209 Tier II
10 NC_000011.10 11 8087890 + TUB NNNGTGTGTGCCTTCCACAA NGG 2 0.2646 Tier II
11 NC_000019.10 19 13128578 - NACC1 NNNGTGTGGCCCTTCCAGAA NGG 2 0.2063 Tier II
12 NC_000017.11 17 73631799 - SDK2 NNNGTGGGTACCTTCCAGAA NAG 2 0.1525 Tier II
13 NC_000003.12 3 38125985 + ACAA1 NNNGTGTGTCTCTTCCAGAA NGG 2 0.1026 Tier II
14 NC_000011.10 11 92305305 - FAT3 NNNGTGTGTACCTTCTAGAA NAG 2 0.0798 Tier II
15 NC_000015.10 15 42587331 - STARD9 NNNGTGTGTTCCTTTCAGAA NGG 2 0.0588 Tier II
16 NC_000021.9 21 34086557 + SLC5A3 NNNGTGTGTATCTTCCTGAA NGG 2 0.041 Tier II
17 NC_000021.9 21 34086557 + MRPS6 NNNGTGTGTATCTTCCTGAA NGG 2 0.041 Tier II
18 NC_000004.12 4 20447089 + SLIT2 NNNGGGTGTACCTTGCAGAA NGG 2 0.032 Tier II
19 NC_000016.10 16 79551053 - MAF NNNGTGTGTAGCTTCCAGAA NGA 2 0.0174 Tier II
20 NC_000010.11 10 70567017 + PALD1 NNNGTGTGTACCTTGCAGAC NGG 2 0.0114 Tier II
21 NC_000021.9 21 42376108 + TMPRSS3 NNNGTGTGTCCCTTCCAGAA NGC 2 0.0074 Tier II
22 NC_000012.12 12 5986189 - VWF NNNGTGTGTACCTTCGAGAA NGC 2 0.0034 Tier II
23 NC_000007.14 7 156804545 - LMBR1 NNNCTGTGTACCTGCCAGAA NGG 2 0.0 Tier II
24 NC_000007.14 7 43485093 - HECW1 NNNGTGTGTACCTTCCAGAA NTT 2 0.0 Tier II
25 NC_000001.11 1 207273241 - LOC107985251 NNNGTATGTACCTACCAGAA NGG 2 0.619 Tier III
26 NC_000001.11 1 207273241 - LINC02942 NNNGTATGTACCTACCAGAA NGG 2 0.619 Tier III
27 NC_000002.12 2 74725983 + LOC102724497 NNNGTGTTTACCTTCCAGTA NGG 2 0.3365 Tier III
28 NC_000001.11 1 89121563 + LOC112268267 NNNTTGTGTTCCTTCCAGAA NGG 2 0.3209 Tier III
29 NC_000006.12 6 48537851 - LOC107986602 NNNGTGTGTACATTACAGAA NGG 2 0.1587 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 25564479 - Fgfr1 NNNCTGTATACCTTCCAGAA NGG 2 0.5294 Tier I
2 NC_000079.6 13 92683433 - Serinc5 NNNATGTGTACCTTTCAGAA NGG 2 0.06 Tier I
3 NC_000085.6 19 47875047 - Gsto2 NNNGTGTGTACCTTACAGAA NAG 2 0.0576 Tier I
4 NC_000070.6 4 83246765 - Ttc39b NNNGTTTGTACCTTCCAGAA NGG 1 0.6667 Tier II
5 NC_000068.7 2 155716594 - Edem2 NNNGTGAGTAACTTCCAGAA NGG 2 0.6563 Tier II
6 NC_000085.6 19 27227140 - Vldlr NNNGTGACTACCTTCCAGAA NGG 2 0.5385 Tier II
7 NC_000082.6 16 93841744 + Morc3 NNNGTGTGTACCATCCAGAG NGG 2 0.5294 Tier II
8 NC_000075.6 9 86310560 - Ube2cbp NNNGAGTGTACATTCCAGAA NGG 2 0.3571 Tier II
9 NC_000083.6 17 86475614 - Prkce NNNTTGTGCACCTTCCAGAA NGG 2 0.3357 Tier II
10 NC_000072.6 6 51244367 - Gm38821 NNNGTGTGCATCTTCCAGAA NGG 2 0.284 Tier II
11 NC_000082.6 16 45676685 - Tmprss7 NNNGAGTGTACCTTCCAGTA NGG 2 0.2692 Tier II
12 NC_000069.6 3 90574460 + S100a3 NNNGTGAGTACCTTCCAGAA NAG 2 0.2269 Tier II
13 NC_000068.7 2 102139734 - Ldlrad3 NNNGTGTGTATCTTCCAAAA NGG 2 0.213 Tier II
14 NC_000073.6 7 96532332 - Tenm4 NNNGTGTCTACCTTACAGAA NGG 2 0.1368 Tier II
15 NC_000070.6 4 129374430 + Zbtb8a NNNGTGTGTACCTTCCAGCT NGG 2 0.1241 Tier II
16 NC_000079.6 13 20893958 + Aoah NNNGAGTGTACCTTCCAGAA NGA 2 0.0347 Tier II
17 NC_000070.6 4 45897388 + Ccdc180 NNNGTGTGTACCTTACAGAA NGC 2 0.0049 Tier II
18 NC_000075.6 9 62531287 - Coro2b NNNGTGTGTACCTTGCAGAA NTG 2 0.0019 Tier II
19 NC_000085.6 19 27227140 - Gm35438 NNNGTGACTACCTTCCAGAA NGG 2 0.5385 Tier III
20 NC_000072.6 6 145370518 - LOC105247329 NNNGTGTGTACATTCCAGAA NGC 2 0.0159 Tier III
21 NC_000069.6 3 112033391 - Gm6602 NNNCTGTGTACCTTCCAGAA NGC 2 0.0118 Tier III
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Other clones with same target sequence:

(none)