Construct: sgRNA BRDN0001146878

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAATATAGAGAATGACACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2D (817)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77186

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 113517656 + CAMK2D NNNATATAGAGAATGACACC NGG 0 1.0 Tier I
2 NC_000017.11 17 49817908 - KAT7 NNNATATGGAGAATGATACC NGG 2 0.3422 Tier I
3 NC_000001.11 1 216860440 + ESRRG NNNATAAAGAAAATGACACC NGG 2 0.875 Tier II
4 NC_000002.12 2 198130475 - PLCL1 NNNATAAAGAGAGTGACACC NGG 2 0.5707 Tier II
5 NC_000007.14 7 150973268 - KCNH2 NNNATATTGAAAATGACACC NGG 2 0.4286 Tier II
6 NC_000001.11 1 69633167 - LRRC7 NNNATATAAGGAATGACACC NGG 2 0.3571 Tier II
7 NC_000003.12 3 58973633 - CFAP20DC NNNCTATAGAGAATGACACC NGG 1 0.3529 Tier II
8 NC_000003.12 3 44969971 + ZDHHC3 NNNATGTTGAGAATGACACC NGG 2 0.3061 Tier II
9 NC_000019.10 19 51480767 + CEACAM18 NNNATACAGAGCATGACACC NGG 2 0.1809 Tier II
10 NC_000017.11 17 74795507 - TMEM104 NNNATAAAGAGAATGACAGC NGG 2 0.1094 Tier II
11 NC_000004.12 4 73414075 + ALB NNNATATAGAGTCTGACACC NGG 2 0.0702 Tier II
12 NC_000002.12 2 173120192 - MAP3K20 NNNATATATAGAATGACACG NGG 2 0.0314 Tier II
13 NC_000012.12 12 16591912 - MGST1 NNNATATAGAGAATGACAAG NGG 2 0.0252 Tier II
14 NC_000012.12 12 16591912 - LMO3 NNNATATAGAGAATGACAAG NGG 2 0.0252 Tier II
15 NC_000001.11 1 43315827 - TIE1 NNNATATAGAGTATGACACC NTG 2 0.013 Tier II
16 NC_000011.10 11 13852156 - LINC02545 NNNAGAGAGAGAATGACACC NGG 2 0.3765 Tier III
17 NC_000003.12 3 58973633 - CFAP20DC-AS1 NNNCTATAGAGAATGACACC NGG 1 0.3529 Tier III
18 NC_000001.11 1 72667028 - LOC105378797 NNNATATATAGAATGAAACC NGG 2 0.2489 Tier III
19 NC_000002.12 2 84894357 - LOC105374837 NNNCTACAGAGAATGACACC NGG 2 0.2426 Tier III
20 NC_000013.11 13 27367818 + LOC105370126 NNNATTTAGAGTATGACACC NGG 2 0.2381 Tier III
21 NC_000009.12 9 13929270 + LINC00583 NNNATATAGAGCATGACCCC NGG 2 0.0501 Tier III
22 NC_000009.12 9 13929270 + LOC101929507 NNNATATAGAGCATGACCCC NGG 2 0.0501 Tier III
23 NC_000016.10 16 62748184 + LOC102723560 NNNATATAGAGAATGACAAC NTG 2 0.0167 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 20107697 - Dsc1 NNNACATAGAGAAAGACACC NGG 2 0.619 Tier I
2 NC_000067.6 1 128134521 + R3hdm1 NNNACTTAGAGAATGACACC NGG 2 0.7143 Tier II
3 NC_000086.7 X 100367025 - Eda NNNATGTAGAGAATAACACC NGG 2 0.6723 Tier II
4 NC_000082.6 16 12011514 + Shisa9 NNNATATAGAGAATGACTCC NGG 1 0.5 Tier II
5 NC_000079.6 13 38215066 - Snrnp48 NNNATGCAGAGAATGACACC NGG 2 0.4911 Tier II
6 NC_000072.6 6 145156822 - Lrmp NNNATAAAGGGAATGACACC NGG 2 0.4861 Tier II
7 NC_000074.6 8 20900597 + Gm15056 NNNATAAAGAGAATGACACA NGG 2 0.4375 Tier II
8 NC_000071.6 5 103973414 - Hsd17b13 NNNGGATAGAGAATGACACC NGG 2 0.4 Tier II
9 NC_000081.6 15 32401195 - Sema5a NNNATACAGAGAATGACAAC NGG 2 0.2946 Tier II
10 NC_000081.6 15 73518158 + Dennd3 NNNAAAGAGAGAATGACACC NGG 2 0.2941 Tier II
11 NC_000071.6 5 141664594 - Sdk1 NNNATATAGACAATGACACA NGG 2 0.2143 Tier II
12 NC_000073.6 7 26671536 + Cyp2b23 NNNATACAGAGAATCACACC NGG 2 0.1875 Tier II
13 NC_000071.6 5 87003853 + Ugt2b35 NNNATGTAGAGAATGACACC NAG 2 0.1852 Tier II
14 NC_000081.6 15 98220285 + Olfr287 NNNATATAGAGAAACACACC NGG 2 0.1688 Tier II
15 NC_000073.6 7 65660380 - Tarsl2 NNNATTTAGAGACTGACACC NGG 2 0.1504 Tier II
16 NC_000070.6 4 156042924 + Ttll10 NNNATATAGAAAATTACACC NGG 2 0.1429 Tier II
17 NC_000068.7 2 161011825 - Chd6 NNNATATATAGCATGACACC NGG 2 0.1404 Tier II
18 NC_000085.6 19 53961376 - Shoc2 NNNATATAGAGAATCACAAC NGG 2 0.1169 Tier II
19 NC_000075.6 9 34831188 + Kirrel3 NNNATATACAGAATGGCACC NGG 2 0.1036 Tier II
20 NC_000070.6 4 43351540 - Atp8b5 NNNATATAGAGACTGACAAC NGG 2 0.0902 Tier II
21 NC_000075.6 9 117602399 - Rbms3 NNNATATAGAGAAAGACACC NGA 2 0.043 Tier II
22 NC_000078.6 12 79458222 - Rad51b NNNATATAGAGAAAGACACC NGA 2 0.043 Tier II
23 NC_000083.6 17 25167236 + Ccdc154 NNNATAGAGAGAATGAGACC NGG 2 0.0346 Tier II
24 NC_000077.6 11 116811487 + Mxra7 NNNAGATAGAGAATGACACC NTG 2 0.0249 Tier II
25 NC_000080.6 14 103056003 - Acod1 NNNATATAGAGAATGAAACC NTG 2 0.0182 Tier II
26 NC_000069.6 3 67024352 - Rsrc1 NNNATATAGAGAATTACACC NGA 2 0.0099 Tier II
27 NC_000067.6 1 128134521 + Gm29383 NNNACTTAGAGAATGACACC NGG 2 0.7143 Tier III
28 NC_000076.6 10 125913279 + Gm29944 NNNAGATAGAAAATGACACC NGG 2 0.64 Tier III
29 NC_000069.6 3 27811135 - Gm32795 NNNATATAGTTAATGACACC NGG 2 0.3394 Tier III
30 NC_000069.6 3 27811135 - Gm42194 NNNATATAGTTAATGACACC NGG 2 0.3394 Tier III
31 NC_000068.7 2 138659023 - Gm14061 NNNATCTAGAGAAAGACACC NGG 2 0.2814 Tier III
32 NC_000073.6 7 136114759 - Gm51502 NNNATATAGAGAGTGACACC NAG 2 0.1691 Tier III
33 NC_000075.6 9 34831188 + Gm33561 NNNATATACAGAATGGCACC NGG 2 0.1036 Tier III
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Other clones with same target sequence:

(none)